Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 332,751 A→G 7.7% intergenic (‑65/‑111) PP_0273 ← / → PP_5435 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947332,7510AG 7.7% 147.0 / 2.6 52intergenic (‑65/‑111)PP_0273/PP_5435hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base G (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

TTGGCAGGGCAGTACAGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGA  >  NC_002947/332604‑332898
                                                                                                                                                   |                                                                                                                                                    
ttGGCAGGGCAGTACAGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCaa                                                                                                                                                     >  3:281682/1‑149 (MQ=255)
 tGGCAGGGCAGTACAGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAAt                                                                                                                                                    <  3:23310/149‑1 (MQ=255)
  ggcagggcagTACAGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCaa                                                                                                                                                     <  6:86821/147‑1 (MQ=255)
              cAGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCg                                                                                                                                       >  3:258878/1‑149 (MQ=255)
               aGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGc                                                                                                                                      >  7:114313/1‑149 (MQ=255)
               aGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGc                                                                                                                                      >  6:92864/1‑149 (MQ=255)
                            agcagATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTAc                                                                                                                         <  1:109080/149‑1 (MQ=255)
                                 aTTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATcgc                                                                                                                    <  2:144111/149‑1 (MQ=255)
                                  ttCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATcgcg                                                                                                                   <  6:144663/149‑1 (MQ=255)
                                      cTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCATGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGCCGGGCATCAATCCATTGGCGATCGCCAGGTCACCGAACATCCCGCAt                                                                                                                >  5:170979/1‑148 (MQ=255)
                                       tCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATc                                                                                                               >  7:173310/1‑148 (MQ=255)
                                           ggTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGat                                                                                                          <  4:220777/149‑1 (MQ=255)
                                               gCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCt                                                                                                      >  4:195129/1‑149 (MQ=255)
                                               gCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCt                                                                                                      <  3:160516/149‑1 (MQ=255)
                                                cGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTg                                                                                                     <  4:281682/149‑1 (MQ=255)
                                                cGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTg                                                                                                     <  2:219631/149‑1 (MQ=255)
                                                     gTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTg                                                                                                 >  6:310351/1‑148 (MQ=255)
                                                        tgtgGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACa                                                                                              <  3:63406/148‑1 (MQ=255)
                                                             ggAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCGATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑c                                                                                       >  1:127282/1‑149 (MQ=255)
                                                              gAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑Ct                                                                                      >  6:230984/1‑149 (MQ=255)
                                                              gAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑Ct                                                                                      <  7:59070/149‑1 (MQ=255)
                                                               aaTCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCa                                                                                                             <  3:138909/126‑1 (MQ=255)
                                                               aaTCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCa                                                                                                             >  4:138909/1‑126 (MQ=255)
                                                                aTCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGTCAACCAGATAT‑CTgg                                                                                    <  2:89359/149‑1 (MQ=255)
                                                                aTCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGAACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTg                                                                                     >  1:200030/1‑148 (MQ=255)
                                                                     ttGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATc                                                                                 <  5:92864/147‑1 (MQ=255)
                                                                     ttGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATc                                                                                 <  8:114313/147‑1 (MQ=255)
                                                                       ggCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGga                                                                              <  4:182828/148‑1 (MQ=255)
                                                                        gCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGgaga                                                                            <  2:301446/149‑1 (MQ=255)
                                                                                   cctccctcAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCGATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGgaga                                                                            <  2:87452/138‑1 (MQ=255)
                                                                                   cctccctcAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCGATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGgaga                                                                            >  1:87452/1‑138 (MQ=255)
                                                                                      cactcAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTAGTTTGACGGTCATCAATACATTGGCGATCGCCAGGTAACCGTACATCGCGCATCAAGATATATGGTTGACAGATAT‑CTGGATCGGAGATATTTTACACCCt                                                               >  8:115077/3‑148 (MQ=255)
                                                                                        ctcAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATg                                                            >  2:237238/1‑149 (MQ=255)
                                                                                           aGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTg                                                         >  3:287332/1‑149 (MQ=255)
                                                                                                      ttCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCGATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCgg                                                <  2:127282/147‑1 (MQ=255)
                                                                                                        cTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGa                                                                                                <  2:300029/98‑1 (MQ=255)
                                                                                                        cTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGa                                                                                                >  1:300029/1‑98 (MQ=255)
                                                                                                        cTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGac                                             <  6:198948/148‑1 (MQ=255)
                                                                                                        cTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGac                                             >  5:198948/1‑148 (MQ=255)
                                                                                                           aaCGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATATCCTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGac                                             <  1:237238/146‑1 (MQ=255)
                                                                                                           aaCGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGAACGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTTGAACGGAGATATTTTACACCCTATGTAGGTGAGCCGGGacaac                                          >  2:219267/1‑148 (MQ=255)
                                                                                                            aCGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGacaaca                                         >  4:96044/1‑148 (MQ=255)
                                                                                                              gTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGtt                                                          <  4:278836/129‑1 (MQ=255)
                                                                                                              gTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGtt                                                          >  3:278836/1‑129 (MQ=255)
                                                                                                                aaaCTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGGTCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATg                                    >  3:66127/1‑149 (MQ=255)
                                                                                                                 aaCTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGc                                   <  8:173310/149‑1 (MQ=255)
                                                                                                                   ctcctcGCCCAGCCCGTCGTTTGACTGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGAGAACACAATgggg                                 <  2:200030/149‑1 (MQ=255)
                                                                                                                   ctcctcGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCgg                                 <  1:219267/149‑1 (MQ=255)
                                                                                                                   ctcctcGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCgg                                 <  3:195129/149‑1 (MQ=255)
                                                                                                                    tcctcGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCggg                                >  6:41299/1‑149 (MQ=255)
                                                                                                                      ctcGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGacaa                                           <  5:283939/136‑1 (MQ=255)
                                                                                                                      ctcGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGt                               >  4:41842/1‑148 (MQ=255)
                                                                                                                                ccGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGGTCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAg                    <  4:66127/149‑1 (MQ=255)
                                                                                                                                                  cAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGa  >  1:42167/1‑149 (MQ=255)
                                                                                                                                                   aaTCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGa  <  2:65893/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGGCAGGGCAGTACAGGTCGCAACCCCAGCAGATTCGCTCGGGGTGGCGAGGGTTTGTGGGGAATCTCTTGGCCATGCTCATCCTCCCTCAGGGCTGCCTGTTCTGAACGTAAACTCCTCGCCCAGCCCGTCGTTTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATAT‑CTGGATCGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGA  >  NC_002947/332604‑332898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑