Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,399,567 C→A 8.4% R108L (CGA→CTA) ‡ PP_3878 ← minor capsid protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,399,5670CA 8.4% 175.2 / 4.4 56R108L (CGA→CTA) ‡PP_3878minor capsid protein C
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base A (2/3);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCAGCCTGGTA  >  NC_002947/4399423‑4399715
                                                                                                                                                |                                                                                                                                                    
aGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAg                                                                                                                                                  >  4:278362/1‑149 (MQ=255)
aGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAg                                                                                                                                                  <  3:247828/149‑1 (MQ=255)
aGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAg                                                                                                                                                  >  6:228873/1‑149 (MQ=255)
   ccGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGccc                                                                                                                                               <  5:8801/149‑1 (MQ=255)
   ccGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGccc                                                                                                                                               <  8:167088/149‑1 (MQ=255)
         agtGGCTTGATCTGGGTCGCGGCCCGAATGGCCGCCGCCAGTTCAAAAGCGCCGCCGGCGCTGCCGCCGGGGCGGTCAATGTCGAGCACAATGTGTTCGACCATGGGGGCAGGGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATa                                                                                                                                         <  8:82979/147‑1 (MQ=255)
         aTTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCAt                                                                                                                                          <  4:237215/148‑1 (MQ=255)
         aTTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTCCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCAt                                                                                                                                          <  7:71010/148‑1 (MQ=255)
          ttGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCtgaataa                                                                                                                                        <  1:116885/149‑7 (MQ=255)
             gTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTg                                                                                                                                     >  2:171307/1‑149 (MQ=255)
             gTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTg                                                                                                                                     >  2:265319/1‑149 (MQ=255)
              tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCt                                                                                                                                              <  4:80110/139‑1 (MQ=255)
              tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCt                                                                                                                                              >  3:80110/1‑139 (MQ=255)
                tGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCg                                                                                                                                                     >  2:214164/1‑130 (MQ=255)
                tGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCg                                                                                                                                                     <  1:214164/130‑1 (MQ=255)
                       ggTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATg                                                                                                                               <  8:181809/145‑1 (MQ=255)
                       ggTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATg                                                                                                                               <  5:207453/145‑1 (MQ=255)
                           gcgGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCtgaa                                                                                                                                           <  6:230709/129‑4 (MQ=255)
                           gcgGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCtgaa                                                                                                                                           >  5:230709/1‑126 (MQ=255)
                                 cGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTAGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGtt                                                                                                                 >  8:27934/1‑149 (MQ=255)
                                   aaTGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTc                                                                                                                >  8:11769/1‑148 (MQ=255)
                                         cgccgcCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGc                                                                                                         >  1:159967/1‑149 (MQ=255)
                                                  ttCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGAc                                                                                                <  1:92951/149‑1 (MQ=255)
                                                     aaaaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTca                                                                                             <  7:11769/149‑1 (MQ=255)
                                                     aaaaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTca                                                                                             >  7:59479/1‑149 (MQ=255)
                                                     aaaaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTc                                                                                              <  1:161228/148‑1 (MQ=255)
                                                     aaaaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTc                                                                                              <  1:171307/148‑1 (MQ=255)
                                                        aGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACaa                                                                                          <  1:265319/149‑1 (MQ=255)
                                                                 ggCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCAt                                                                                 >  5:34714/1‑149 (MQ=255)
                                                                      tGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACg                                                                            >  3:320379/1‑149 (MQ=255)
                                                                        ccgccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACg                                                                            <  6:236676/147‑1 (MQ=255)
                                                                           ccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGaa                                                                                       <  5:263884/133‑1 (MQ=255)
                                                                           ccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGaa                                                                                       >  6:263884/1‑133 (MQ=255)
                                                                                 cTGTCAATGTAGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTAGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTc                                                                 <  7:27934/149‑1 (MQ=255)
                                                                                    tCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGa                                                                        <  2:156849/139‑1 (MQ=255)
                                                                                                  aaTGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGa                                                <  4:320379/149‑1 (MQ=255)
                                                                                                            aCCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGgcggcgcg                                      >  6:306519/1‑149 (MQ=255)
                                                                                                               aTGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGcggcgcggcg                                   <  5:228873/149‑1 (MQ=255)
                                                                                                                tGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCggcgcggcgc                                  >  3:27094/1‑149 (MQ=255)
                                                                                                                 ggggTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCt                                 >  6:197195/1‑149 (MQ=255)
                                                                                                                  gggTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCt                                 <  6:34714/148‑1 (MQ=255)
                                                                                                                    gTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGtt                              <  4:27094/149‑1 (MQ=255)
                                                                                                                         tGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTcg                         >  2:66563/1‑149 (MQ=255)
                                                                                                                           aTGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTcgcg                       <  3:278362/149‑1 (MQ=255)
                                                                                                                              gCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCgcgg                      <  1:66563/147‑1 (MQ=255)
                                                                                                                               ccTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGa                                                >  4:316915/1‑120 (MQ=255)
                                                                                                                               ccTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGa                                                <  3:316915/120‑1 (MQ=255)
                                                                                                                                cTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGAt                  >  6:134205/1‑149 (MQ=255)
                                                                                                                                cTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGAt                  <  5:306519/149‑1 (MQ=255)
                                                                                                                                cTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGAt                  >  5:221214/1‑149 (MQ=255)
                                                                                                                                    ttCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTg              >  7:293154/1‑149 (MQ=255)
                                                                                                                                      cAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTcg                         <  3:231963/136‑1 (MQ=255)
                                                                                                                                      cAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTcg                         >  4:231963/1‑136 (MQ=255)
                                                                                                                                       aGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCt            >  4:61630/1‑148 (MQ=255)
                                                                                                                                       aGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTc           <  5:197195/149‑1 (MQ=255)
                                                                                                                                        gggCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCt            >  4:89387/1‑147 (MQ=255)
                                                                                                                                                cGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGTCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCAGCCTGGTa  <  5:38576/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCAGCCTGGTA  >  NC_002947/4399423‑4399715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑