Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,405,314	C→T	8.2%	G83R (GGG→AGG)	PP_3886 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,405,314	0	C	T	8.2%	206.1 / 7.2	73	G83R (GGG→AGG)	PP_3886	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/33);  new base T (3/3);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

ACAACCGATAGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAG  >  NC_002947/4405168‑4405458                                                                                                                                                   |                                                                                                                                                  aCAACCGATAGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCg                                                                                                                                                 <  8:37995/149‑1 (MQ=255)          aGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGcc                                                                                                                                        >  5:242287/1‑149 (MQ=255)            ccAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGcc                                                                                                                                        >  2:147456/1‑147 (MQ=255)            ccAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGa                                                                                                                                      <  3:138209/149‑1 (MQ=255)              aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                    >  8:252612/1‑149 (MQ=255)              aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                    >  7:73380/1‑149 (MQ=255)              aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                    >  6:159741/1‑149 (MQ=255)                  aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                 <  1:294910/148‑1 (MQ=255)                  aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                 >  2:294910/1‑148 (MQ=255)                  aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTg                                                                                                                                <  8:73380/149‑1 (MQ=255)                   aCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                 <  5:198450/147‑1 (MQ=255)                       ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGGGCtttttcg                                                                                                                            <  4:144874/148‑6 (MQ=255)                       ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGGGCtttttc                                                                                                                             <  8:246428/147‑5 (MQ=255)                       ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAAt                                                                                                                            <  2:62934/148‑1 (MQ=255)                        cTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATgg                                                                                                                          <  2:178609/149‑1 (MQ=255)                           ggAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATggg                                                                                                                         <  3:42720/147‑1 (MQ=255)                           ggAACAGGGCGACCCGAGTTCAGACGATGACCCGTGAAGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGt                                                                                                                        <  6:273960/148‑1 (MQ=255)                            gAACAGGGCGCCCCGAGTTCCGCCGACGACCCGTCACGGAAGTTGGAGGGACTGCCCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTgg                                                                                                                      <  8:173517/149‑1 (MQ=255)                            gAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                       >  6:243696/1‑148 (MQ=255)                                  ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                       <  2:199992/142‑1 (MQ=255)                                  ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                       >  1:199992/1‑142 (MQ=255)                                       cccGAGTTCCGCCGATGACCGGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTTACGAAATAGCGCCGGCATGTGCCGAGGCTTGGAATGGGTGGATA‑TGGCAAcc                                                                                                          <  2:29352/149‑1 (MQ=255)                                           aGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGt                                                                                                      <  7:24678/149‑1 (MQ=255)                                             ttCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTc                                                                                                     >  4:33541/1‑148 (MQ=255)                                               ccgccgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAg                                                                                                   <  1:323383/148‑1 (MQ=255)                                                cgccgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGt                                                                                                 >  5:184747/1‑149 (MQ=255)                                                          ccGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGgaga                                                                                                                   >  1:212542/1‑122 (MQ=255)                                                          ccGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGgaga                                                                                                                   <  2:212542/122‑1 (MQ=255)                                                             tGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGgcg                                                                                    <  1:51390/149‑1 (MQ=255)                                                                 ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTATCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGTGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTc                                                                                >  5:264833/1‑149 (MQ=255)                                                                 ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                    <  6:304746/98‑1 (MQ=255)                                                                 ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                    >  5:304746/1‑98 (MQ=255)                                                                     tttGGAGGGACTGGGCAGAAATCTGAGCATCTATCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGTGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTc                                                                            <  6:264833/149‑1 (MQ=255)                                                                     tttGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTc                                                                            >  2:25085/1‑149 (MQ=255)                                                                        ggAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCa                                                                          <  1:147456/148‑1 (MQ=255)                                                                            ggACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTccc                                                                     <  2:131859/149‑1 (MQ=255)                                                                             gACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTccc                                                                     >  2:17437/1‑148 (MQ=255)                                                                                   gCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCt                                                               >  6:94361/1‑148 (MQ=255)                                                                                    cAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGa                                                             <  3:33541/149‑1 (MQ=255)                                                                                     ggAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAAcc                                                                                                          >  2:121913/2‑103 (MQ=255)                                                                                     ggAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAAcc                                                                                                          <  1:121913/102‑1 (MQ=255)                                                                                     aGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGAc                                                            <  5:243696/149‑1 (MQ=255)                                                                                          tCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCACCACGCTGACTGTTa                                                       >  3:241012/1‑149 (MQ=255)                                                                                            tGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCACCGGCATGTGCAGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTAGCTGTCGTGGCGCTACCCTCCAGTACCCAAGCTGACTGTTAcc                                                     >  6:169025/1‑149 (MQ=255)                                                                                             gAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTAccc                                                    >  7:147956/1‑149 (MQ=255)                                                                                                  tCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCATGCTGACTGTTACCCCTGCa                                               >  1:258316/1‑149 (MQ=255)                                                                                                   cTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTcc                                                                               <  4:174261/116‑1 (MQ=255)                                                                                                   cTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTcc                                                                               >  3:174261/1‑116 (MQ=255)                                                                                                    tAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACg                                             >  6:195051/1‑149 (MQ=255)                                                                                                    tAACGTCTCGACGCTATCCATGACTGGCTCAATTTAACGAAAAAGCCCCGGCATGTGACGAGGCTTGGAATGGGTTGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTACCTACAGTCACCACGCTGAATTTTACCCCttaacg                                             >  7:313577/1‑144 (MQ=255)                                                                                                        gTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTgc                                         >  5:303305/1‑149 (MQ=255)                                                                                                                    tgcATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGTTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCtt                               <  5:159741/145‑1 (MQ=255)                                                                                                                    tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                              <  1:25085/148‑1 (MQ=255)                                                                                                                    tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                              <  5:94361/148‑1 (MQ=255)                                                                                                                    tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                              >  5:58714/1‑148 (MQ=255)                                                                                                                      cATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGat                           <  7:252612/149‑1 (MQ=255)                                                                                                                      cATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGa                            <  6:58714/148‑1 (MQ=255)                                                                                                                       aTGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatc                          <  6:184747/149‑1 (MQ=255)                                                                                                                          aCTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                       >  7:21244/1‑149 (MQ=255)                                                                                                                               cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGAGTGCCAACCCTTACAGTACCATGTCGTGGCGCTTCCATCCGGTCCCAACGATCAATTTAACCCCTCCCCGTTGCCCCCGGCGTTTGATAATATcc                    >  1:259320/1‑148 (MQ=255)                                                                                                                               cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCa                   >  2:154229/1‑148 (MQ=255)                                                                                                                               cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCa                   >  2:159345/1‑148 (MQ=255)                                                                                                                                  cATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCaaaaa               >  5:329936/1‑149 (MQ=255)                                                                                                                                   aTTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc              <  8:21244/149‑1 (MQ=255)                                                                                                                                    tttAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc              >  6:84232/1‑148 (MQ=255)                                                                                                                                    tttAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCACTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc              >  6:306563/1‑148 (MQ=255)                                                                                                                                      tAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGa            >  2:333231/1‑148 (MQ=255)                                                                                                                                       aaCGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGAc           <  5:84232/148‑1 (MQ=255)                                                                                                                                          gAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCACTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAg       <  5:306563/149‑1 (MQ=255)                                                                                                                                            ccaaGCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGcc     >  2:331221/3‑149 (MQ=255)                                                                                                                                                gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                       >  5:115672/1‑127 (MQ=255)                                                                                                                                                gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                       <  6:115672/127‑1 (MQ=255)                                                                                                                                                gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTCTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAg  >  8:203881/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ACAACCGATAGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAG  >  NC_002947/4405168‑4405458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑