Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,405,320	T→C	8.0%	M81V (ATG→GTG)	PP_3886 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,405,320	0	T	C	8.0%	210.6 / 7.3	75	M81V (ATG→GTG)	PP_3886	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base C (3/3);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

AGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGGGT  >  NC_002947/4405177‑4405464                                                                                                                                                |                                                                                                                                                  aGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGcc                                                                                                                                              >  5:242287/1‑149 (MQ=255)   ccAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGcc                                                                                                                                              >  2:147456/1‑147 (MQ=255)   ccAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGa                                                                                                                                            <  3:138209/149‑1 (MQ=255)     aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                          >  8:252612/1‑149 (MQ=255)     aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                          >  6:159741/1‑149 (MQ=255)     aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                          >  7:73380/1‑149 (MQ=255)         aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                       >  2:294910/1‑148 (MQ=255)         aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                       <  1:294910/148‑1 (MQ=255)         aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTg                                                                                                                                      <  8:73380/149‑1 (MQ=255)          aCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                       <  5:198450/147‑1 (MQ=255)              ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGGGCtttttcg                                                                                                                                  <  4:144874/148‑6 (MQ=255)              ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGGGCtttttc                                                                                                                                   <  8:246428/147‑5 (MQ=255)              ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAAt                                                                                                                                  <  2:62934/148‑1 (MQ=255)               cTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATgg                                                                                                                                <  2:178609/149‑1 (MQ=255)                  ggAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATggg                                                                                                                               <  3:42720/147‑1 (MQ=255)                  ggAACAGGGCGACCCGAGTTCAGACGATGACCCGTGAAGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGt                                                                                                                              <  6:273960/148‑1 (MQ=255)                   gAACAGGGCGCCCCGAGTTCCGCCGACGACCCGTCACGGAAGTTGGAGGGACTGCCCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTgg                                                                                                                            <  8:173517/149‑1 (MQ=255)                   gAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                             >  6:243696/1‑148 (MQ=255)                         ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                             <  2:199992/142‑1 (MQ=255)                         ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                             >  1:199992/1‑142 (MQ=255)                              cccGAGTTCCGCCGATGACCGGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTTACGAAATAGCGCCGGCATGTGCCGAGGCTTGGAATGGGTGGATA‑TGGCAAcc                                                                                                                <  2:29352/149‑1 (MQ=255)                                  aGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGt                                                                                                            <  7:24678/149‑1 (MQ=255)                                    ttCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTc                                                                                                           >  4:33541/1‑148 (MQ=255)                                      ccgccgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAg                                                                                                         <  1:323383/148‑1 (MQ=255)                                       cgccgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGt                                                                                                       >  5:184747/1‑149 (MQ=255)                                                 ccGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGgaga                                                                                                                         >  1:212542/1‑122 (MQ=255)                                                 ccGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGgaga                                                                                                                         <  2:212542/122‑1 (MQ=255)                                                    tGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGgcg                                                                                          <  1:51390/149‑1 (MQ=255)                                                        ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTATCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGTGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTc                                                                                      >  5:264833/1‑149 (MQ=255)                                                        ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                          >  5:304746/1‑98 (MQ=255)                                                        ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                          <  6:304746/98‑1 (MQ=255)                                                            tttGGAGGGACTGGGCAGAAATCTGAGCATCTATCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGTGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTc                                                                                  <  6:264833/149‑1 (MQ=255)                                                            tttGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTc                                                                                  >  2:25085/1‑149 (MQ=255)                                                               ggAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCa                                                                                <  1:147456/148‑1 (MQ=255)                                                                   ggACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTccc                                                                           <  2:131859/149‑1 (MQ=255)                                                                    gACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTccc                                                                           >  2:17437/1‑148 (MQ=255)                                                                          gCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCt                                                                     >  6:94361/1‑148 (MQ=255)                                                                           cAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGa                                                                   <  3:33541/149‑1 (MQ=255)                                                                            ggAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAAcc                                                                                                                <  1:121913/102‑1 (MQ=255)                                                                            ggAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAAcc                                                                                                                >  2:121913/2‑103 (MQ=255)                                                                            aGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGAc                                                                  <  5:243696/149‑1 (MQ=255)                                                                                 tCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCACCACGCTGACTGTTa                                                             >  3:241012/1‑149 (MQ=255)                                                                                   tGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCACCGGCATGTGCAGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTAGCTGTCGTGGCGCTACCCTCCAGTACCCAAGCTGACTGTTAcc                                                           >  6:169025/1‑149 (MQ=255)                                                                                    gAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTAccc                                                          >  7:147956/1‑149 (MQ=255)                                                                                         tCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCATGCTGACTGTTACCCCTGCa                                                     >  1:258316/1‑149 (MQ=255)                                                                                          cTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTcc                                                                                     <  4:174261/116‑1 (MQ=255)                                                                                          cTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTcc                                                                                     >  3:174261/1‑116 (MQ=255)                                                                                           tAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACg                                                   >  6:195051/1‑149 (MQ=255)                                                                                           tAACGTCTCGACGCTATCCATGACTGGCTCAATTTAACGAAAAAGCCCCGGCATGTGACGAGGCTTGGAATGGGTTGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTACCTACAGTCACCACGCTGAATTTTACCCCttaacg                                                   >  7:313577/1‑144 (MQ=255)                                                                                               gTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTgc                                               >  5:303305/1‑149 (MQ=255)                                                                                                           tgcATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGTTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCtt                                     <  5:159741/145‑1 (MQ=255)                                                                                                           tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                                    <  5:94361/148‑1 (MQ=255)                                                                                                           tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                                    >  5:58714/1‑148 (MQ=255)                                                                                                           tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                                    <  1:25085/148‑1 (MQ=255)                                                                                                             cATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGat                                 <  7:252612/149‑1 (MQ=255)                                                                                                             cATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGa                                  <  6:58714/148‑1 (MQ=255)                                                                                                              aTGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatc                                <  6:184747/149‑1 (MQ=255)                                                                                                                 aCTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                             >  7:21244/1‑149 (MQ=255)                                                                                                                      cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGAGTGCCAACCCTTACAGTACCATGTCGTGGCGCTTCCATCCGGTCCCAACGATCAATTTAACCCCTCCCCGTTGCCCCCGGCGTTTGATAATATcc                          >  1:259320/1‑148 (MQ=255)                                                                                                                      cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCa                         >  2:154229/1‑148 (MQ=255)                                                                                                                      cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCa                         >  2:159345/1‑148 (MQ=255)                                                                                                                         cATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCaaaaa                     >  5:329936/1‑149 (MQ=255)                                                                                                                          aTTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc                    <  8:21244/149‑1 (MQ=255)                                                                                                                           tttAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc                    >  6:84232/1‑148 (MQ=255)                                                                                                                           tttAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCACTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc                    >  6:306563/1‑148 (MQ=255)                                                                                                                             tAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGa                  >  2:333231/1‑148 (MQ=255)                                                                                                                              aaCGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGAc                 <  5:84232/148‑1 (MQ=255)                                                                                                                                 gAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCACTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAg             <  5:306563/149‑1 (MQ=255)                                                                                                                                   ccaaGCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGcc           >  2:331221/3‑149 (MQ=255)                                                                                                                                       gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                             <  6:115672/127‑1 (MQ=255)                                                                                                                                       gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                             >  5:115672/1‑127 (MQ=255)                                                                                                                                       gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTCTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAg        >  8:203881/1‑148 (MQ=255)                                                                                                                                           cGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑GTGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTggg   <  6:242287/149‑1 (MQ=255)                                                                                                                                            ggCATGTGCCGATGCTTGGAATGGGTGGAGA‑TGTCAACCCTGTCAGGTCGCTGTCGTTGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGGCGCCTGGCTTTGATCATCTCCAAAAACTACAAAGCCCAGCTTGGt  <  5:169025/149‑1 (MQ=255)                                                                                                                                            ggCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCGTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCATGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGGGt  <  2:258316/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  AGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGGGT  >  NC_002947/4405177‑4405464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑