Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,405,327	A→G	8.3%	P78P (CCT→CCC)	PP_3886 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,405,327	0	A	G	8.3%	200.8 / 6.1	73	P78P (CCT→CCC)	PP_3886	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/33);  new base G (3/3);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGGGTGGCCGGGCTT  >  NC_002947/4405179‑4405474                                                                                                                                                     |                                                                                                                                                     ccAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGa                                                                                                                                                      <  3:138209/149‑1 (MQ=255)   aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                                    >  8:252612/1‑149 (MQ=255)   aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                                    >  6:159741/1‑149 (MQ=255)   aGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                                    >  7:73380/1‑149 (MQ=255)       aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                                 >  2:294910/1‑148 (MQ=255)       aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                                 <  1:294910/148‑1 (MQ=255)       aaCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTg                                                                                                                                                <  8:73380/149‑1 (MQ=255)        aCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCtt                                                                                                                                                 <  5:198450/147‑1 (MQ=255)            ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGGGCtttttcg                                                                                                                                            <  4:144874/148‑6 (MQ=255)            ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGGGCtttttc                                                                                                                                             <  8:246428/147‑5 (MQ=255)            ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAAt                                                                                                                                            <  2:62934/148‑1 (MQ=255)             cTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATgg                                                                                                                                          <  2:178609/149‑1 (MQ=255)                ggAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATggg                                                                                                                                         <  3:42720/147‑1 (MQ=255)                ggAACAGGGCGACCCGAGTTCAGACGATGACCCGTGAAGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGt                                                                                                                                        <  6:273960/148‑1 (MQ=255)                 gAACAGGGCGCCCCGAGTTCCGCCGACGACCCGTCACGGAAGTTGGAGGGACTGCCCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTgg                                                                                                                                      <  8:173517/149‑1 (MQ=255)                 gAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                                       >  6:243696/1‑148 (MQ=255)                       ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                                       <  2:199992/142‑1 (MQ=255)                       ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTg                                                                                                                                       >  1:199992/1‑142 (MQ=255)                            cccGAGTTCCGCCGATGACCGGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTTACGAAATAGCGCCGGCATGTGCCGAGGCTTGGAATGGGTGGATA‑TGGCAAcc                                                                                                                          <  2:29352/149‑1 (MQ=255)                                aGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGt                                                                                                                      <  7:24678/149‑1 (MQ=255)                                  ttCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTc                                                                                                                     >  4:33541/1‑148 (MQ=255)                                    ccgccgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAg                                                                                                                   <  1:323383/148‑1 (MQ=255)                                     cgccgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGt                                                                                                                 >  5:184747/1‑149 (MQ=255)                                               ccGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGgaga                                                                                                                                   >  1:212542/1‑122 (MQ=255)                                               ccGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGgaga                                                                                                                                   <  2:212542/122‑1 (MQ=255)                                                  tGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGgcg                                                                                                    <  1:51390/149‑1 (MQ=255)                                                      ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTATCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGTGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTc                                                                                                >  5:264833/1‑149 (MQ=255)                                                      ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                                    >  5:304746/1‑98 (MQ=255)                                                      ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAgg                                                                                                                                                    <  6:304746/98‑1 (MQ=255)                                                          tttGGAGGGACTGGGCAGAAATCTGAGCATCTATCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGTGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTc                                                                                            <  6:264833/149‑1 (MQ=255)                                                          tttGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTc                                                                                            >  2:25085/1‑149 (MQ=255)                                                             ggAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCa                                                                                          <  1:147456/148‑1 (MQ=255)                                                                 ggACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTccc                                                                                     <  2:131859/149‑1 (MQ=255)                                                                  gACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTccc                                                                                     >  2:17437/1‑148 (MQ=255)                                                                        gCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCt                                                                               >  6:94361/1‑148 (MQ=255)                                                                         cAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGa                                                                             <  3:33541/149‑1 (MQ=255)                                                                          ggAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAAcc                                                                                                                          >  2:121913/2‑103 (MQ=255)                                                                          ggAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAAcc                                                                                                                          <  1:121913/102‑1 (MQ=255)                                                                          aGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGAc                                                                            <  5:243696/149‑1 (MQ=255)                                                                               tCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCACCACGCTGACTGTTa                                                                       >  3:241012/1‑149 (MQ=255)                                                                                 tGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCACCGGCATGTGCAGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTAGCTGTCGTGGCGCTACCCTCCAGTACCCAAGCTGACTGTTAcc                                                                     >  6:169025/1‑149 (MQ=255)                                                                                  gAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTAccc                                                                    >  7:147956/1‑149 (MQ=255)                                                                                       tCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCATGCTGACTGTTACCCCTGCa                                                               >  1:258316/1‑149 (MQ=255)                                                                                        cTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTcc                                                                                               <  4:174261/116‑1 (MQ=255)                                                                                        cTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTcc                                                                                               >  3:174261/1‑116 (MQ=255)                                                                                         tAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACg                                                             >  6:195051/1‑149 (MQ=255)                                                                                         tAACGTCTCGACGCTATCCATGACTGGCTCAATTTAACGAAAAAGCCCCGGCATGTGACGAGGCTTGGAATGGGTTGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTACCTACAGTCACCACGCTGAATTTTACCCCttaacg                                                             >  7:313577/1‑144 (MQ=255)                                                                                             gTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTgc                                                         >  5:303305/1‑149 (MQ=255)                                                                                                         tgcATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGTTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCtt                                               <  5:159741/145‑1 (MQ=255)                                                                                                         tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                                              <  1:25085/148‑1 (MQ=255)                                                                                                         tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                                              >  5:58714/1‑148 (MQ=255)                                                                                                         tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                                              <  5:94361/148‑1 (MQ=255)                                                                                                           cATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGat                                           <  7:252612/149‑1 (MQ=255)                                                                                                           cATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGa                                            <  6:58714/148‑1 (MQ=255)                                                                                                            aTGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatc                                          <  6:184747/149‑1 (MQ=255)                                                                                                               aCTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                                       >  7:21244/1‑149 (MQ=255)                                                                                                                    cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGAGTGCCAACCCTTACAGTACCATGTCGTGGCGCTTCCATCCGGTCCCAACGATCAATTTAACCCCTCCCCGTTGCCCCCGGCGTTTGATAATATcc                                    >  1:259320/1‑148 (MQ=255)                                                                                                                    cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCa                                   >  2:159345/1‑148 (MQ=255)                                                                                                                    cTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCa                                   >  2:154229/1‑148 (MQ=255)                                                                                                                       cATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCaaaaa                               >  5:329936/1‑149 (MQ=255)                                                                                                                        aTTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc                              <  8:21244/149‑1 (MQ=255)                                                                                                                         tttAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc                              >  6:84232/1‑148 (MQ=255)                                                                                                                         tttAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCACTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc                              >  6:306563/1‑148 (MQ=255)                                                                                                                           tAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGa                            >  2:333231/1‑148 (MQ=255)                                                                                                                            aaCGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGAc                           <  5:84232/148‑1 (MQ=255)                                                                                                                               gAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCACTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAg                       <  5:306563/149‑1 (MQ=255)                                                                                                                                 ccaaGCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGcc                     >  2:331221/3‑149 (MQ=255)                                                                                                                                     gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                                       <  6:115672/127‑1 (MQ=255)                                                                                                                                     gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcatc                                       >  5:115672/1‑127 (MQ=255)                                                                                                                                     gCCTCGGCACATGCCGGGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTCTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAg                  >  8:203881/1‑148 (MQ=255)                                                                                                                                         cGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑GTGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTggg             <  6:242287/149‑1 (MQ=255)                                                                                                                                          ggCATGTGCCGATGCTTGGAATGGGTGGAGA‑TGTCAACCCTGTCAGGTCGCTGTCGTTGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGGCGCCTGGCTTTGATCATCTCCAAAAACTACAAAGCCCAGCTTGGt            <  5:169025/149‑1 (MQ=255)                                                                                                                                          ggCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCGTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCATGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGGGt            <  2:258316/149‑1 (MQ=255)                                                                                                                                                    gAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGGGTGGCCGGGCtt  >  1:125960/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGGCTTGGAATGGGTGGAGA‑TGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGGGTGGCCGGGCTT  >  NC_002947/4405179‑4405474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑