Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 334,603 A→T 8.5% L28* (TTG→TAG) ‡ PP_0276 ← Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947334,6030AT 8.5% 131.1 / 3.2 47L28* (TTG→TAG) ‡PP_0276Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (27/16);  new base T (2/2);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

TGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGT  >  NC_002947/334460‑334749
                                                                                                                                                |                                                                                                                                                   
tGTCCATCATGGAGTCCAATGTTAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTcc                                                                                                                                                <  4:79606/149‑1 (MQ=255)
  tCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCAt                                                                                                                                              <  1:115768/149‑1 (MQ=255)
      tcatGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCA‑TTTTTTGCTGGAGGGAGTTATTAA‑AATGGTCAAGTGCAttttg                                                                                                                                          >  8:178029/1‑147 (MQ=255)
        atGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACTTGTCCATTTTCat                                                                                                                                        <  5:47630/149‑2 (MQ=255)
                     tGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTg                                                                                                                          >  3:119461/1‑149 (MQ=255)
                       aaTTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCA‑TTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCg                                                                                                                       >  6:113969/1‑149 (MQ=255)
                            ctcGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTAt                                                                                                                   >  6:322310/1‑149 (MQ=255)
                               gagcTTGCAAATGGCAAGATCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTCTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTg                                                                                                                >  8:2367/4‑149 (MQ=255)
                                agattGCCATTTTCAAGCTCGGATATGCGGTATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGc                                                                                                               <  7:285315/146‑1 (MQ=255)
                                      ccATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCgtg                                                                                                         >  3:123207/1‑149 (MQ=255)
                                            gCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTTCGTCGTGGGTGGc                                                                                                   >  7:228066/1‑149 (MQ=255)
                                                 cTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCgtgg                                                                                                        >  1:337938/1‑139 (MQ=255)
                                                 cTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCgtgg                                                                                                        <  2:337938/139‑1 (MQ=255)
                                                     gATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAgg                                                                                          >  2:138762/1‑149 (MQ=255)
                                                          gCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCtt                                                                                      >  8:91766/1‑148 (MQ=255)
                                                           cGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTc                                                                                    >  2:185517/1‑149 (MQ=255)
                                                                    gTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAAtt                                                                            >  4:198943/1‑148 (MQ=255)
                                                                    gTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTg                                                                           <  3:198943/149‑1 (MQ=255)
                                                                        cGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAg                                                                        <  8:184159/148‑1 (MQ=255)
                                                                         gTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgcg                                                                      >  4:204602/1‑149 (MQ=255)
                                                                           tCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgagc                                                                     >  7:261920/1‑148 (MQ=255)
                                                                              gCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATc                                                                 <  3:146004/149‑1 (MQ=255)
                                                                                  ggCTGCGGCATCATGCTGTTTGAGGCCCA‑TTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGc                                                              <  5:113969/147‑1 (MQ=255)
                                                                                          catcatGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCgg                                                     >  7:241758/1‑149 (MQ=255)
                                                                                          catcatGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCgg                                                     <  1:185517/149‑1 (MQ=255)
                                                                                              atGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAg                                                  >  4:284941/1‑148 (MQ=255)
                                                                                               tGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGa                                                 >  7:209346/1‑148 (MQ=255)
                                                                                               tGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTAGGCAGa                                                 >  7:106714/1‑148 (MQ=255)
                                                                                                    tttGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAGAATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGt                                                                                                 >  1:56368/1‑96 (MQ=255)
                                                                                                    tttGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGt                                                                                                 <  2:56368/95‑1 (MQ=255)
                                                                                                        cggCCCA‑TTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTaaa                                       <  7:178029/147‑1 (MQ=255)
                                                                                                           cccATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCtt                                                            >  8:174529/1‑125 (MQ=255)
                                                                                                           cccATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCtt                                                            <  7:174529/125‑1 (MQ=255)
                                                                                                                  ttttGATGCAGTCAGTGATGAA‑AATTGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTaaaa                                      <  8:241042/140‑1 (MQ=255)
                                                                                                                   tttGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATa                             >  3:225164/1‑148 (MQ=255)
                                                                                                                        tGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCg                        <  8:241758/148‑1 (MQ=255)
                                                                                                                           agtcagtGATGAA‑AATGGACAAGTCCATTTTCAAGGCGCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAg                     >  1:61163/1‑149 (MQ=255)
                                                                                                                              cagtGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGATTTAGTGGAATTGAAGCGCCATCGGATTGCCTCGGAAGAAATATCTAAAATTTGCGATAAATCGTAGtgtg                 >  8:193678/1‑149 (MQ=255)
                                                                                                                               agtGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGtgtg                 >  2:258035/1‑148 (MQ=255)
                                                                                                                                gtGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGa               <  1:138762/149‑1 (MQ=255)
                                                                                                                                 tgatgaA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTCCCTCGGCAGAAAGATCTAAAATTTGCGATAAATCGTACTGAGGAt              >  4:253206/1‑149 (MQ=255)
                                                                                                                                   ttgaA‑AATGGACTTGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAATTCGTAGTGTGGAttg            >  2:145192/2‑149 (MQ=255)
                                                                                                                                   ttgaA‑AATGGACTTGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttg            >  2:69529/2‑149 (MQ=255)
                                                                                                                                     gaA‑AATGGACTTGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttg            <  1:145192/147‑1 (MQ=255)
                                                                                                                                     gaA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTATGGAttggt          >  3:148177/1‑149 (MQ=255)
                                                                                                                                         aaTGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGGGTGGAttggttgg       >  2:62330/1‑149 (MQ=255)
                                                                                                                                              aCAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGt  <  3:284941/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAA‑AATGGACAAGTCCATTTTCAAGGC‑CATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGT  >  NC_002947/334460‑334749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑