Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,459,104 C→T 5.9% F43F (TTC→TTT)  trkH‑I → potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,459,1040CT 5.9% 202.9 / 2.1 67F43F (TTC→TTT) trkH‑Ipotassium transporter
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base T (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

TCCCAACGAGCTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAA  >  NC_002947/4458958‑4459249
                                                                                                                                                  |                                                                                                                                                   
tCCCAACGAGCTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTc                                                                                                                                                     <  3:126540/147‑1 (MQ=255)
  ccAACGAGCTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑c                                                                                                                                                 <  7:173690/148‑1 (MQ=255)
  ccAACGAGCTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑Ct                                                                                                                                                >  8:30951/1‑149 (MQ=255)
   cAACGAGCTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGGGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTg                                                                                                                                               <  8:178670/149‑1 (MQ=255)
    aaCGAGCTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTgg                                                                                                                                              >  8:286060/1‑149 (MQ=255)
          cTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTc                                                                                                                                            >  7:308915/1‑145 (MQ=255)
          agcccTCAATGCCGCTTCCTTCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCTTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTc                                                                                                                                            <  8:308915/143‑1 (MQ=255)
             ccTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGCCGACCTCGGTGCATTCGT‑CTGGTCGAGCCt                                                                                                                                      >  4:304061/1‑148 (MQ=255)
             ccTAAATGCCGCTTCCTGCATTGCGTTTCCTAGACTTCATTATTGGTGTATTCCGGATCACGTTGTCCGTCAGCATGGTGATTCCAATGCTGCCGCTGCTGGTGTACGACAGCAGCGACGACCTCGGTGAATTCGT‑CTGGTCGAGCCt                                                                                                                                      >  4:309000/1‑148 (MQ=255)
              cTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTTGT‑CTGGTCGAGCCTGa                                                                                                                                    >  7:181341/1‑149 (MQ=255)
                 aaTGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATc                                                                                                                                  <  7:296697/148‑1 (MQ=255)
                     ccGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTc                                                                                                                              <  3:182902/148‑1 (MQ=255)
                      cGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGtt                                                                                                                               <  8:97984/146‑1 (MQ=255)
                      cGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGtt                                                                                                                               >  7:97984/1‑146 (MQ=255)
                       gCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCtgt                                                                                                                           <  1:19721/149‑1 (MQ=255)
                             tGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCg                                                                                                                     >  1:189873/1‑149 (MQ=255)
                                aTTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCATCATGGTGATTCCACTGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGGGTGTCGGcc                                                                                                                  <  8:7718/149‑1 (MQ=255)
                                 ttGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCt                                                                                                                 <  6:126305/149‑1 (MQ=255)
                                       ttCATTGACTTCATTATTGGTGTATTGCTTTTCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCa                                                                                                           <  7:130900/148‑1 (MQ=255)
                                        tCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCtgt                                                                                                                           >  8:166077/1‑132 (MQ=255)
                                        tCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCAt                                                                                                          <  2:46625/148‑1 (MQ=255)
                                         cATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCGGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCAtg                                                                                                         <  8:139441/148‑1 (MQ=255)
                                          aTCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCAtgg                                                                                                        <  4:143599/148‑1 (MQ=255)
                                                ttCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCcg                                                                                                 >  6:35285/1‑149 (MQ=255)
                                                ttCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCcg                                                                                                 >  2:255003/1‑149 (MQ=255)
                                                    ttattGGTGTAATCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGGGGCCCgcggc                                                                                             <  5:262078/149‑1 (MQ=255)
                                                        tGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTa                                                                                          <  6:242263/148‑1 (MQ=255)
                                                          gtgtATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTAccc                                                                                       >  3:243146/1‑149 (MQ=255)
                                                            gtATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGa                                                                                     >  1:317137/1‑149 (MQ=255)
                                                             tATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACTCGat                                                                                    <  2:50959/149‑2 (MQ=255)
                                                               ttCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTg                                                                                                                                               <  6:111142/89‑1 (MQ=255)
                                                                  cTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTc                                                                                                                                            <  1:340988/89‑1 (MQ=255)
                                                                  cTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTc                                                                                                                                            >  2:340988/1‑89 (MQ=255)
                                                                  cTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACAcc                                                                               >  2:328381/1‑149 (MQ=255)
                                                                         cGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTg                                                                        >  7:296907/1‑149 (MQ=255)
                                                                           ttGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCg                                                                      <  8:296907/149‑1 (MQ=255)
                                                                              gCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGa                                                                    <  2:189873/148‑1 (MQ=255)
                                                                               ccGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTTGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGAcc                                                                  <  8:181341/149‑1 (MQ=255)
                                                                                cGTCAGCATTTTGATTCCAATGCTGACGCTGCTTGTGTAAGACCGCAGCGACGACGTCGGTGCATTCGT‑CTGGTCGAGCCTTATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGACCGCGGCGTACCCGACACACCGCAACTGCGGACCa                                                                 <  3:49157/149‑1 (MQ=255)
                                                                                cGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCa                                                                 <  2:317137/149‑1 (MQ=255)
                                                                                     gCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGa                                                             >  2:334645/1‑148 (MQ=255)
                                                                                      cATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGAc                                                            <  1:255003/149‑1 (MQ=255)
                                                                                       aTGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACAt                                                          <  7:286060/149‑1 (MQ=255)
                                                                                        tGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATg                                                         >  5:281905/1‑149 (MQ=255)
                                                                                               tCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGc                                                  >  4:222432/1‑149 (MQ=255)
                                                                                                 cAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGCCACACCGCAACTGCGGACCAGGGACATGTATCTGCTc                                                >  7:272766/1‑149 (MQ=255)
                                                                                                 cAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACAt                                                          >  2:173182/1‑139 (MQ=255)
                                                                                                 cAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACAt                                                          <  1:173182/139‑1 (MQ=255)
                                                                                                 cAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCt                                                 >  6:337330/1‑148 (MQ=255)
                                                                                                 cAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTc                                                <  1:328381/149‑1 (MQ=255)
                                                                                                  aaTGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCa                                               >  6:165878/1‑149 (MQ=255)
                                                                                                  aaTGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGATCa                                               >  6:176488/1‑149 (MQ=255)
                                                                                                      cTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTGCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCacga                                            >  1:75320/1‑149 (MQ=255)
                                                                                                       tGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTTGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGa                                                             >  6:309063/1‑130 (MQ=255)
                                                                                                       tGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTTGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGa                                                             <  5:309063/130‑1 (MQ=255)
                                                                                                       tGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCacgacg                                          >  3:205319/1‑149 (MQ=255)
                                                                                                          cGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCa                                       <  5:337330/149‑1 (MQ=255)
                                                                                                               ctgGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTg                                  >  3:1080/1‑149 (MQ=255)
                                                                                                                  gtgtACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTgg                                 <  6:130731/147‑1 (MQ=255)
                                                                                                                  gtgtACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGt                               >  7:294873/1‑149 (MQ=255)
                                                                                                                         acagCAGCGACGACCTCGTTTCATTCTT‑CTGGTCGAGCCTGTTCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTAGCCGAAAGACGGCAAGTGCGGACCTGGGAAATTTATCTGCTCACGACGGCAAGCTGGCTCATCgtg                        <  8:272766/146‑1 (MQ=255)
                                                                                                                           cGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGtgtg                      >  6:129815/1‑149 (MQ=255)
                                                                                                                            gCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTgc                     <  4:205319/149‑1 (MQ=255)
                                                                                                                                       cTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCgcggc          >  2:292289/1‑149 (MQ=255)
                                                                                                                                            tgccgtcgt‑cgGGTCGAGCCTGCTCGGGCTGTGGGGCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACGGCGGACCAGGGACATGCCGCTGCGCACGACGGCAAGCTGGGTCATCGGGTGCGCATGCGCGGCGCTGc     <  3:309000/144‑1 (MQ=255)
                                                                                                                                            tGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGc     <  4:243146/149‑1 (MQ=255)
                                                                                                                                            tGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGc     <  3:304061/149‑1 (MQ=255)
                                                                                                                                              cATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGcc    >  4:108029/1‑148 (MQ=255)
                                                                                                                                               aTTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCaa  <  4:1080/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCCCAACGAGCTCCCTCAATGCCGCTTCCTGCATTGCGTTTCATCGCCTTCATTATTGGTGTATTCCTGATCACGTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGT‑CTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTG‑CTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAA  >  NC_002947/4458958‑4459249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑