Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,463,925	T→A	8.8%	L130* (TTG→TAG)	PP_3956 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,463,925	0	T	A	8.8%	152.1 / 2.8	53	L130* (TTG→TAG)	PP_3956	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/28);  new base A (3/2);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCCTTGCACGCCGGGCGAACGCCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCG  >  NC_002947/4463780‑4464071                                                                                                                                                   |                                                                                                                                                         cAGCCCTTGCACGCCGGGCGAACGCCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGa                                                                                                                                                       <  4:293078/149‑1 (MQ=255)       ttGCACGCCGGGCGAACGCCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGAcagcag                                                                                                                                                 >  3:109520/1‑149 (MQ=255)         gCACGCCGGGCGAACGCCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAgg                                                                                                                                                <  1:150087/148‑1 (MQ=255)          cACGCCGGGCGAACGCCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGc                                                                                                                                               <  4:317029/148‑1 (MQ=255)                 ggCGAACGCCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCgc‑‑c                                                                                                                                     <  8:298180/149‑1 (MQ=255)                    gAACGCCCGAGGCT‑GTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCgc‑‑c‑ctgc                                                                                                                                >  2:246304/1‑149 (MQ=255)                                cTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGa                                                                                                                     <  8:82811/149‑1 (MQ=255)                                    tGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑gg                                                                                                                <  6:14381/149‑1 (MQ=255)                                    tGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑g                                                                                                                 <  1:246304/148‑1 (MQ=255)                                     gACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑ggg                                                                                                               >  2:275709/1‑149 (MQ=255)                                       cTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑ggg                                                                                                               >  2:191079/1‑147 (MQ=255)                                        tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑Ag                                                                                                           >  8:48777/1‑149 (MQ=255)                                         aCCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑Ag                                                                                                           >  3:254592/1‑148 (MQ=255)                                          ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGTCGCCGTCA‑GGGC‑Agg                                                                                                          <  1:62407/148‑1 (MQ=255)                                          ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGa                                                                                                         >  8:275762/1‑149 (MQ=255)                                           cAGGTCTGCACGTCTGACGGGTGCCAGCCACTGTCCTAAGGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGAc                                                                                                        <  1:284882/149‑1 (MQ=255)                                              gTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCg                                                                                                      >  3:108245/1‑148 (MQ=255)                                                      gACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACtt                                                                                            <  8:46479/149‑1 (MQ=255)                                                         aGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCAc                                                                                              >  1:70256/1‑144 (MQ=255)                                                         aGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCAc                                                                                              <  2:70256/144‑1 (MQ=255)                                                                   cagccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGt                                                                               <  5:170788/149‑1 (MQ=255)                                                                       caCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCaa                                                                           <  4:109520/149‑1 (MQ=255)                                                                        aCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCGAAATCCACTTCAGCCCACCACGTGCaa                                                                           <  7:48777/149‑1 (MQ=255)                                                                                 ttGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑AGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAgg                                                                 >  2:258741/1‑149 (MQ=255)                                                                                 ttGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑AGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAgg                                                                 >  2:262988/1‑149 (MQ=255)                                                                                   ggTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGt                                                                <  5:176304/148‑1 (MQ=255)                                                                                    gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTAAGGAAAGCAGGCGAACCCC‑‑C‑CTGCCCTGACGGCCACA‑GGGC‑AGCACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATACAGGTg                                                               >  1:271350/1‑149 (MQ=255)                                                                                    gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTgg                                                              <  2:77153/149‑1 (MQ=255)                                                                                    gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTgg                                                              >  2:290018/1‑149 (MQ=255)                                                                                         gACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTc                                                         <  8:184204/149‑1 (MQ=255)                                                                                          aCCGCCGAGCTAACGAATTGCTGCAGTAGACTTCTGGGCTTGTTAAAATCCGCTTT‑TGTCCAGCAGGCGAACCCCTACACTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGt                                                           <  1:275709/149‑1 (MQ=255)                                                                                            cgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGc                                                                    <  6:308699/135‑1 (MQ=255)                                                                                            cgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTgg                                                              <  3:63799/141‑1 (MQ=255)                                                                                            cgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTgg                                                              >  4:63799/1‑141 (MQ=255)                                                                                              ccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑AGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACATGGCC‑TGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTAc                                                     <  1:262988/149‑1 (MQ=255)                                                                                              ccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑AGGACAGCAGGCGAAACGC‑‑C‑CTGCCCTGACGGCGACA‑GGGCTTGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTAc                                                     <  1:258741/149‑1 (MQ=255)                                                                                               cgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCcca                                                                                     <  7:306576/115‑1 (MQ=255)                                                                                               cgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCcca                                                                                     >  8:306576/1‑115 (MQ=255)                                                                                               cgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTAc                                                     <  1:290018/147‑1 (MQ=255)                                                                                                  gCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATc                                                >  5:144475/1‑149 (MQ=255)                                                                                                    gAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTAc                                                     <  4:254540/142‑1 (MQ=255)                                                                                                    gAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTAc                                                     >  3:254540/1‑142 (MQ=255)                                                                                                       cGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGa                                           <  5:317388/149‑1 (MQ=255)                                                                                                         cATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGCATg                                         >  4:120926/1‑149 (MQ=255)                                                                                                          aTTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGt                                        >  7:10712/1‑149 (MQ=255)                                                                                                                 cAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAAt                                 >  6:345284/1‑149 (MQ=255)                                                                                                                 cAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAAt                                 <  7:305766/149‑1 (MQ=255)                                                                                                                         ttCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGt                         >  6:184137/1‑149 (MQ=255)                                                                                                                         ttCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGt                         >  7:216943/1‑149 (MQ=255)                                                                                                                                        aaTCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCa          <  2:271350/149‑1 (MQ=255)                                                                                                                                         aTCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCat         <  8:216943/149‑1 (MQ=255)                                                                                                                                          tCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCatg        <  8:10712/149‑1 (MQ=255)                                                                                                                                            cGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCatgat      >  2:74546/1‑149 (MQ=255)                                                                                                                                                 t‑tGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCAACCCCATGATGTCg  >  6:9878/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                         CAGCCCTTGCACGCCGGGCGAACGCCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTT‑TGGACAGCAGGCGAACCGC‑‑C‑CTGCCCTGACGGCGACA‑GGGC‑AGGACCG‑AATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCG  >  NC_002947/4463780‑4464071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑