Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,477,814 G→C 6.7% Q60E (CAG→GAG) ‡ PP_3968 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,477,8140GC 6.7% 179.4 / 3.4 60Q60E (CAG→GAG) ‡PP_3968sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (30/25);  new base C (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

CGATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCA  >  NC_002947/4477668‑4477960
                                                                                                                                                  |                                                                                                                                                  
cGATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGAt                                                                                                                                                  <  4:220968/149‑1 (MQ=255)
 gATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATg                                                                                                                                                 <  8:95281/149‑1 (MQ=255)
 gATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATg                                                                                                                                                 <  3:320389/149‑1 (MQ=255)
 gATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCACGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATg                                                                                                                                                 >  2:16037/1‑149 (MQ=255)
       acacTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGt                                                                                                                                           <  3:293542/149‑1 (MQ=255)
           tCGAGAGCAGCGCTCGGTACTCAGAGACCAATTGATCAATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGTTCTGAGCCGCCGTATCGATTCTCATTAGAGGGCCTATGACTTGCGCCTTCTCAGACTGCTGAAGCGCAGTCt                                                                                                                                         >  8:313579/1‑147 (MQ=255)
           ccGAGCGCTGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGAAATGGCATGGGTCTGAGCCGCCGTATCGCGGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTgc                                                                                                                                       >  7:212072/2‑148 (MQ=255)
                          ggTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCatat                                                                                                                          <  6:532/147‑1 (MQ=255)
                             aCTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCg                                                                                                                     >  8:104116/1‑149 (MQ=255)
                                 agagACCAATTGATCCGTCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGTCaa                                                                                                                 >  8:219288/1‑149 (MQ=255)
                                   agaCCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACg                                                                                                               >  2:295891/1‑149 (MQ=255)
                                   agaCCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGAGGCAACg                                                                                                               >  6:290816/1‑149 (MQ=255)
                                     aCCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGt                                                                                                             <  7:104116/149‑1 (MQ=255)
                                             aTCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGaa                                                                                                     >  8:124703/1‑149 (MQ=255)
                                                cATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtct                                                                                                  >  5:132594/1‑149 (MQ=255)
                                                 aTCGAAAAGCGCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtctt                                                                                                 <  1:16037/149‑1 (MQ=255)
                                                 aTCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGCGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACCGTGCGAAGAAtct                                                                                                  >  8:128847/1‑148 (MQ=255)
                                                 aTCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCTCAGAAtctt                                                                                                 <  6:132594/149‑1 (MQ=255)
                                                    gAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGAATCTATGCTAAATAGAGGGCCTATGCATTGCTCCTTCTCAGACTGCTGATGCGCCGTCGGAGCAGTCCGCATATCAGCGGACACGGTGCGCAGAAtcttct                                                                                               >  6:252266/1‑148 (MQ=255)
                                                       aaGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCaga                                                                                           <  7:124703/149‑1 (MQ=255)
                                                          cccGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGa                                                                                                                                       >  6:18585/1‑102 (MQ=255)
                                                          cccGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGa                                                                                                                                       <  5:18585/102‑1 (MQ=255)
                                                           ccGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTATAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATAAGAGGAAACGGTTCGCAGAATCTTATCAGATTGa                                                                                       >  8:233199/1‑149 (MQ=255)
                                                            cGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGa                                                                                                                                       <  1:143234/100‑1 (MQ=255)
                                                            cGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGa                                                                                                                                       >  2:143234/1‑100 (MQ=255)
                                                                 tCAAACGAGTAATGGCATGGGTCTGAGCCGACGTATCGCTGCTCATTAGAGTGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCt                                                                                  <  7:313579/148‑1 (MQ=255)
                                                                   aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGa                                                                                <  6:46671/148‑1 (MQ=255)
                                                                              ggCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGc                                                                                                                              >  8:179910/1‑91 (MQ=255)
                                                                              ggCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGc                                                                                                                              <  7:179910/91‑1 (MQ=255)
                                                                               gCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATc                                                                   >  8:20264/1‑149 (MQ=255)
                                                                                cATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCACTGCCTTGCGCATTCTCAGACTGCTGATGAGCAGTCTGAGCAGTACGCATATCAGCTGAAACGGTGCGCATAAACTTCTCAGAGTGATACCTGAGGACGGCTCaaac                                                                   >  8:136133/1‑146 (MQ=255)
                                                                                 aTGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCt                                                                 >  3:15398/1‑149 (MQ=255)
                                                                                      tCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTg                                                            <  8:212072/149‑1 (MQ=255)
                                                                                          aGCCGCCTTATCGCTGATCATTATAGGGCCTATGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTAAGCATATCAGCGGCAACGTTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCAGTCTGAATCCTGAGTgg                                                           <  5:252266/146‑1 (MQ=255)
                                                                                                     cGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTg                                                            >  4:258801/1‑134 (MQ=255)
                                                                                                     cGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTg                                                            <  3:258801/134‑1 (MQ=255)
                                                                                                         gctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGAcc                                          >  6:205159/1‑148 (MQ=255)
                                                                                                           tCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCCCAGTCTGAGCAGTACGCATATCAGCCGCAACGGTGCGCAGAATCTTCACAGAGTCATTCCAGAGGCCCCCTGAATCCTGACTGGGTAAGTCCGTCTAGACCGaa                                       >  3:227087/1‑149 (MQ=255)
                                                                                                             aTTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATc                                                                   >  7:266274/1‑119 (MQ=255)
                                                                                                             aTTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATc                                                                   <  8:266274/119‑1 (MQ=255)
                                                                                                                 gagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                  <  7:219288/148‑1 (MQ=255)
                                                                                                                  agGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGa                                <  1:104796/149‑1 (MQ=255)
                                                                                                                       ctctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcc                           <  1:295891/149‑1 (MQ=255)
                                                                                                                       ctctGCCTTGCGCCTTCTCAGACTGCCGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTggg                                                          <  4:266845/118‑1 (MQ=255)
                                                                                                                       ctctGCCTTGCGCCTTCTCAGACTGCCGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTggg                                                          >  3:266845/1‑118 (MQ=255)
                                                                                                                        tctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcct                          >  1:191600/1‑149 (MQ=255)
                                                                                                                           gCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcctcc                        >  4:279264/1‑148 (MQ=255)
                                                                                                                            ccTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTccc                       >  6:295917/1‑148 (MQ=255)
                                                                                                                              ttGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAg                    >  6:168037/1‑149 (MQ=255)
                                                                                                                                 cgcCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCt                 <  5:168037/149‑1 (MQ=255)
                                                                                                                                  gcCTTCTCAGACTGCTTATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTg                <  7:128847/149‑1 (MQ=255)
                                                                                                                                  gcCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACAGAAAGTGCGAAATCCTCCCACGCATg                >  4:240298/1‑149 (MQ=255)
                                                                                                                                    cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTg                >  4:135819/1‑147 (MQ=255)
                                                                                                                                    cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTg                <  3:135819/147‑1 (MQ=255)
                                                                                                                                         cAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGt                                                     <  3:282740/105‑1 (MQ=255)
                                                                                                                                         cAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGt                                                     >  4:282740/1‑105 (MQ=255)
                                                                                                                                          aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGaa           <  5:205159/146‑1 (MQ=255)
                                                                                                                                          aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGc        >  4:53933/1‑149 (MQ=255)
                                                                                                                                            aCTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCt                                                                                  <  2:205922/73‑1 (MQ=255)
                                                                                                                                            aCTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCt                                                                                  >  1:205922/1‑73 (MQ=255)
                                                                                                                                              tgctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTcc    >  6:71903/1‑149 (MQ=255)
                                                                                                                                                ctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCa  >  1:44161/1‑149 (MQ=255)
                                                                                                                                                 tgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAACCCTGGAGAATGCTTCCCa  >  2:169400/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCA  >  NC_002947/4477668‑4477960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑