Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,500,179 C→T 8.3% G295R (GGA→AGA)  PP_3989 ← DNA‑cytosine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,500,1790CT 8.3% 130.5 / 2.7 48G295R (GGA→AGA) PP_3989DNA‑cytosine methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (24/20);  new base T (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

AAAACATTTGGCCAGTGCTGCTGCCACAGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCATTGTCCAATGATTAGG  >  NC_002947/4500033‑4500326
                                                                                                                                                  |                                                                                                                                                   
aaaaCATTTGGCCAGTGCTGCTGCCACAGGTGGAGGCACAGCGTTAGAAATTTGCTAAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTAGTATATACTAATCCTTCTATGTCCAt                                                                                                                                                   >  5:124763/1‑149 (MQ=255)
  aaCATTTGGCCAGTGCTGCTGCCACAGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCAt                                                                                                                                                   >  6:14897/1‑147 (MQ=255)
     aTTTGGCCAGTGCTGCTGCCACAGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGa                                                                                                                                              >  2:40222/1‑149 (MQ=255)
             aGTGCTGCTGCCACAGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACAt                                                                                                                                       >  1:38654/1‑148 (MQ=255)
                       ccACAGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCGTTCTATGTCCATCAGGATGAACATGAATCTCCCt                                                                                                                             <  2:183738/148‑1 (MQ=255)
                       ccACAGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTg                                                                                                                            >  4:323423/1‑149 (MQ=255)
                          caGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTa                                                                                                                         <  5:14897/149‑1 (MQ=255)
                            ggTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTAcc                                                                                                                       <  3:14822/149‑1 (MQ=255)
                                gAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAg                                                                                                                   >  3:226727/1‑149 (MQ=255)
                                  ggCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGc                                                                                                                 >  6:266450/1‑149 (MQ=255)
                                           ttAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCg                                                                                                        >  2:77288/1‑149 (MQ=255)
                                                  tttGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCTATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGt                                                                                                 >  7:239835/1‑149 (MQ=255)
                                                  tttGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCTATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGt                                                                                                 >  5:220339/1‑149 (MQ=255)
                                                      cTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGttttt                                                                                             <  3:292376/149‑1 (MQ=255)
                                                       ttAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGtttttt                                                                                            >  7:145333/1‑149 (MQ=255)
                                                       ttAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGttttt                                                                                             >  4:207848/1‑148 (MQ=255)
                                                       ttAACTTGGGCGCTCAAACTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCACTGTTACCATAGGCCACCGTCAGGCTAGGtttttt                                                                                            >  4:94875/1‑149 (MQ=255)
                                                         aaCTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGtttttt                                                                                            <  5:266450/147‑1 (MQ=255)
                                                          aCTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTTCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCa                                                                                         <  1:57575/149‑1 (MQ=255)
                                                          aCTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTTCCATAGGCCACCGTCCGGCTAGGTTTTTTCCa                                                                                         <  1:40222/149‑1 (MQ=255)
                                                           cTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCACCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCAc                                                                                        <  5:231856/149‑1 (MQ=255)
                                                              gggCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCActc                                                                                      >  2:285613/1‑148 (MQ=255)
                                                                      aaaGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCTATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAAt                                                                             <  6:220339/149‑1 (MQ=255)
                                                                      aaaGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCTATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAAt                                                                             <  8:239835/149‑1 (MQ=255)
                                                                            cccATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGa                                                                       <  6:72465/149‑1 (MQ=255)
                                                                                         aGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTa                                                                                                                         >  7:313186/1‑86 (MQ=255)
                                                                                         aGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTa                                                                                                                         <  8:313186/86‑1 (MQ=255)
                                                                                           cTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCgg                                                        >  4:187541/1‑149 (MQ=255)
                                                                                                tGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCAtc                                                    >  1:178292/1‑148 (MQ=255)
                                                                                                   cgaTCTCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCAc                                                <  3:70411/147‑1 (MQ=255)
                                                                                                     aTCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCa                                              <  3:323423/149‑1 (MQ=255)
                                                                                                       cccTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGt                                            <  4:226727/149‑1 (MQ=255)
                                                                                                        ccTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGt                                            <  1:285613/148‑1 (MQ=255)
                                                                                                         cTGCAGCTTCATTGCCTGGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGGTAGGTGTTATCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCa                                          <  6:124763/149‑1 (MQ=255)
                                                                                                          tGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGCCCACCACCAGTCATCTCAc                                                >  4:70411/1‑142 (MQ=255)
                                                                                                              gCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAAt                                     >  8:114102/1‑149 (MQ=255)
                                                                                                                   aTTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGttt                                <  1:77288/149‑1 (MQ=255)
                                                                                                                            tatataCTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAgg                       <  2:38654/149‑1 (MQ=255)
                                                                                                                               ataCTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGttt                    >  8:164047/1‑149 (MQ=255)
                                                                                                                                   tAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGttt                                >  6:125706/1‑133 (MQ=255)
                                                                                                                                   tAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGttt                                <  5:125706/133‑1 (MQ=255)
                                                                                                                                   tAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCAtt                >  5:195351/1‑149 (MQ=255)
                                                                                                                                   tAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCAtt                >  4:193858/1‑149 (MQ=255)
                                                                                                                                    aaGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCAGTTACCATAGGCCACCGTCCGGCTAGGTTATTTCCACTCTAACTTAATGAACGAGCGACCACCAACCTGCAGCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCAttt               >  4:142016/1‑148 (MQ=255)
                                                                                                                                     aGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAg                        >  2:230313/1‑139 (MQ=255)
                                                                                                                                     aGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAg                        <  1:230313/139‑1 (MQ=255)
                                                                                                                                        cTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCATTGTcc            <  7:114102/148‑1 (MQ=255)
                                                                                                                                            tATGTCCATCAGGATGAACATGAATATCCCTGTTACCATAGGCCACCGTCAGGCTAGGTTTTTTCCACTCTAACTTAATGAAAGAGCGACAACCAACCGTCATCTCACCAGTAAGAAATAGTTTGGACTTAGGTTTCATTGTCAAATGa       >  3:312693/1‑149 (MQ=255)
                                                                                                                                                  cATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCATTGTCCAATGATTAgg  <  8:145333/148‑1 (MQ=255)
                                                                                                                                                  cATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCATTGTCCAATGATTAgg  <  3:193858/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAAACATTTGGCCAGTGCTGCTGCCACAGGTGGAGGCACAGCGTTAGAAATTTGCTTAACTTGGGCGCTCAAAGTCCCCATCAATCTATAGCTGTCTGGGAATCCCTGCAGCTTCATTGCCTCGTATATACTAAGCCTTCTATGTCCATCAGGATGAACATGAATCTCCCTGTTACCATAGGCCACCGTCCGGCTAGGTTTTTTCCACTCTAACTTAATGAACGAGCGACCACCAACCGGCATCTCACCAGTCAGAAATCGTTTGGACTTAGGTTTCATTGTCCAATGATTAGG  >  NC_002947/4500033‑4500326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑