Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,518,706 T→C 6.0% K272E (AAG→GAG)  clpA ← ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,518,7060TC 6.0% 198.8 / 2.7 67K272E (AAG→GAG) clpAATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base T (27/36);  new base C (2/2);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

ATTTCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTG  >  NC_002947/4518561‑4518851
                                                                                                                                                 |                                                                                                                                                 
aTTTCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGaa                                                                                                                                                <  4:13021/149‑1 (MQ=255)
 tttCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAg                                                                                                                                               <  3:204663/149‑1 (MQ=255)
     aCCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCt                                                                                                                                           <  4:61206/149‑1 (MQ=255)
      cccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCtt                                                                                                                                          <  6:68070/149‑1 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                         <  7:113908/149‑1 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                         >  1:150798/1‑149 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                         >  3:241953/1‑149 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                         >  3:174621/1‑149 (MQ=255)
            gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                    <  3:311805/149‑1 (MQ=255)
            gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                    <  2:159084/149‑1 (MQ=255)
            gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGCATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                    >  3:86403/1‑149 (MQ=255)
              cagcagCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                  <  4:238330/149‑1 (MQ=255)
               agcagcGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGAAGCGCTTCAAGGCGCTGCTCggtgag                                                                                                                                 <  8:58673/149‑6 (MQ=255)
                gcagcGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGAAGCGCTTCAAGGCGCTGCTCggtgagc                                                                                                                                <  8:162565/149‑7 (MQ=255)
                 cagcGGCTTGAGCAGTTTGGAGGCATCCATCACGCCGCCCGACTCCGCACCGGCGCCGATGATGGTGTGTATTTCGTCGATGAACAGGATGTCCTGCGGACGCTTGCGCAGCTCACCGAGGAGCGCCTTGATGCGCTTCTCGAAGTcac                                                                                                                               <  4:86403/149‑1 (MQ=255)
                     ggCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACg                                                                                                                           <  7:244370/149‑1 (MQ=255)
                      gCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACgg                                                                                                                          <  3:172041/149‑1 (MQ=255)
                            gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTAttt                                                                                                                     <  2:219452/148‑1 (MQ=255)
                            gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTg                                                                                                                    >  3:223947/1‑149 (MQ=255)
                            gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTg                                                                                                                    <  3:221564/149‑1 (MQ=255)
                             cAGGTTGGAGGCATCCATCACCCCGCCAGTCGCCGCACCGGCGCCGATGTTGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                   <  4:13457/149‑1 (MQ=255)
                                    gAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                            >  8:105077/1‑149 (MQ=255)
                                     aGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                            >  6:306800/1‑148 (MQ=255)
                                      ggCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                            >  2:8337/1‑147 (MQ=255)
                                       gCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAg                                                                                                         <  1:138726/149‑1 (MQ=255)
                                         atccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGc                                                                                                        >  5:74359/1‑148 (MQ=255)
                                         atccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGc                                                                                                        >  2:285842/1‑148 (MQ=255)
                                          tccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                      <  5:12272/149‑1 (MQ=255)
                                            catcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAgg                                                                                                    <  4:268392/149‑1 (MQ=255)
                                             atcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                   >  3:240830/1‑133 (MQ=255)
                                             atcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                   <  4:240830/133‑1 (MQ=255)
                                                     gcccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                      >  3:45456/1‑138 (MQ=255)
                                                     gcccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                      <  4:45456/138‑1 (MQ=255)
                                                      cccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGAt                                                                                           >  1:131901/1‑148 (MQ=255)
                                                      cccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGCCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGAt                                                                                           >  1:112676/1‑148 (MQ=255)
                                                          aCGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAgg                                                                                      <  4:241953/149‑1 (MQ=255)
                                                          aCGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAgg                                                                                      <  7:105077/149‑1 (MQ=255)
                                                            gccgcACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGa                                                                                    <  1:285842/149‑1 (MQ=255)
                                                              cgcACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAg                                                                                   >  2:159808/1‑148 (MQ=255)
                                                                cACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTa                                                                                 >  8:32672/1‑148 (MQ=255)
                                                                              atgGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTgcgc                                                                  <  6:74359/149‑1 (MQ=255)
                                                                                  tgtgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagc                                                              <  2:150798/149‑1 (MQ=255)
                                                                                    tgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagcag                                                            <  5:306800/149‑1 (MQ=255)
                                                                                    tgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagca                                                             <  1:159808/148‑1 (MQ=255)
                                                                                        tttCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCa                                                        <  7:317168/149‑1 (MQ=255)
                                                                                           cgtcgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAgg                                                      >  1:304115/1‑148 (MQ=255)
                                                                                            gtcgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCa                                                    >  3:152202/1‑149 (MQ=255)
                                                                                              cgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCAcc                                                  >  6:131261/1‑149 (MQ=255)
                                                                                                    aCAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                  >  4:48921/1‑63 (MQ=255)
                                                                                                    aCAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                  <  3:48921/63‑1 (MQ=255)
                                                                                                            gCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATcgacga                                     <  4:152202/148‑1 (MQ=255)
                                                                                                            gCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGAt                                    >  2:6050/1‑149 (MQ=255)
                                                                                                             ccTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATg                                   >  3:200792/1‑149 (MQ=255)
                                                                                                              cTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGc                                  <  4:222854/149‑1 (MQ=255)
                                                                                                                gCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGt                                >  4:322766/1‑149 (MQ=255)
                                                                                                                 cGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGt                                <  4:200792/148‑1 (MQ=255)
                                                                                                                               gCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCg                 <  2:131901/149‑1 (MQ=255)
                                                                                                                               gCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCg                 <  8:231871/149‑1 (MQ=255)
                                                                                                                                 tCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGc               >  3:222963/1‑149 (MQ=255)
                                                                                                                                  cACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTc                                                         <  3:115185/106‑1 (MQ=255)
                                                                                                                                  cACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTc                                                         >  4:115185/1‑106 (MQ=255)
                                                                                                                                   ttcGAGAAGCGCTTCAAGGCGCTGCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGa             >  5:57794/3‑149 (MQ=255)
                                                                                                                                   ttcGAGAAGCGCTTCAAGGCGCTGCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGa             >  1:84369/3‑149 (MQ=255)
                                                                                                                                     cGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATg           <  4:222963/149‑1 (MQ=255)
                                                                                                                                         cagcGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCg       >  8:288165/1‑149 (MQ=255)
                                                                                                                                             gcCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCtt   <  7:32672/149‑1 (MQ=255)
                                                                                                                                              ccTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTg  >  2:334467/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATTTCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTG  >  NC_002947/4518561‑4518851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑