Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,518,709 A→G 6.1% F271L (TTC→CTC)  clpA ← ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,518,7090AG 6.1% 190.5 / 2.2 66F271L (TTC→CTC) clpAATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base A (27/34);  new base G (2/2);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

ATTTCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTGCCCA  >  NC_002947/4518561‑4518855
                                                                                                                                                    |                                                                                                                                                  
aTTTCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGaa                                                                                                                                                    <  4:13021/149‑1 (MQ=255)
 tttCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAg                                                                                                                                                   <  3:204663/149‑1 (MQ=255)
     aCCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCt                                                                                                                                               <  4:61206/149‑1 (MQ=255)
      cccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCtt                                                                                                                                              <  6:68070/149‑1 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             >  3:174621/1‑149 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             >  1:150798/1‑149 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             >  3:241953/1‑149 (MQ=255)
       ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             <  7:113908/149‑1 (MQ=255)
            gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                        <  2:159084/149‑1 (MQ=255)
            gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                        <  3:311805/149‑1 (MQ=255)
            gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGCATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                        >  3:86403/1‑149 (MQ=255)
              cagcagCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                      <  4:238330/149‑1 (MQ=255)
               agcagcGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGAAGCGCTTCAAGGCGCTGCTCggtgag                                                                                                                                     <  8:58673/149‑6 (MQ=255)
                gcagcGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGAAGCGCTTCAAGGCGCTGCTCggtgagc                                                                                                                                    <  8:162565/149‑7 (MQ=255)
                 cagcGGCTTGAGCAGTTTGGAGGCATCCATCACGCCGCCCGACTCCGCACCGGCGCCGATGATGGTGTGTATTTCGTCGATGAACAGGATGTCCTGCGGACGCTTGCGCAGCTCACCGAGGAGCGCCTTGATGCGCTTCTCGAAGTcac                                                                                                                                   <  4:86403/149‑1 (MQ=255)
                     ggCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACg                                                                                                                               <  7:244370/149‑1 (MQ=255)
                      gCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACgg                                                                                                                              <  3:172041/149‑1 (MQ=255)
                            gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTAttt                                                                                                                         <  2:219452/148‑1 (MQ=255)
                            gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTg                                                                                                                        >  3:223947/1‑149 (MQ=255)
                            gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTg                                                                                                                        <  3:221564/149‑1 (MQ=255)
                             cAGGTTGGAGGCATCCATCACCCCGCCAGTCGCCGCACCGGCGCCGATGTTGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                       <  4:13457/149‑1 (MQ=255)
                                    gAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                                >  8:105077/1‑149 (MQ=255)
                                     aGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                                >  6:306800/1‑148 (MQ=255)
                                      ggCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                                >  2:8337/1‑147 (MQ=255)
                                       gCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAg                                                                                                             <  1:138726/149‑1 (MQ=255)
                                         atccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGc                                                                                                            >  2:285842/1‑148 (MQ=255)
                                         atccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGc                                                                                                            >  5:74359/1‑148 (MQ=255)
                                          tccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                          <  5:12272/149‑1 (MQ=255)
                                            catcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAgg                                                                                                        <  4:268392/149‑1 (MQ=255)
                                             atcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                       >  3:240830/1‑133 (MQ=255)
                                             atcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                       <  4:240830/133‑1 (MQ=255)
                                                     gcccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                          <  4:45456/138‑1 (MQ=255)
                                                     gcccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                          >  3:45456/1‑138 (MQ=255)
                                                      cccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGAt                                                                                               >  1:131901/1‑148 (MQ=255)
                                                      cccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGCCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGAt                                                                                               >  1:112676/1‑148 (MQ=255)
                                                          aCGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAgg                                                                                          <  7:105077/149‑1 (MQ=255)
                                                          aCGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAgg                                                                                          <  4:241953/149‑1 (MQ=255)
                                                            gccgcACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGa                                                                                        <  1:285842/149‑1 (MQ=255)
                                                              cgcACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAg                                                                                       >  2:159808/1‑148 (MQ=255)
                                                                cACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTa                                                                                     >  8:32672/1‑148 (MQ=255)
                                                                              atgGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTgcgc                                                                      <  6:74359/149‑1 (MQ=255)
                                                                                  tgtgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagc                                                                  <  2:150798/149‑1 (MQ=255)
                                                                                    tgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagcag                                                                <  5:306800/149‑1 (MQ=255)
                                                                                    tgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagca                                                                 <  1:159808/148‑1 (MQ=255)
                                                                                        tttCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCa                                                            <  7:317168/149‑1 (MQ=255)
                                                                                           cgtcgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAgg                                                          >  1:304115/1‑148 (MQ=255)
                                                                                            gtcgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCa                                                        >  3:152202/1‑149 (MQ=255)
                                                                                              cgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCAcc                                                      >  6:131261/1‑149 (MQ=255)
                                                                                                    aCAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                      >  4:48921/1‑63 (MQ=255)
                                                                                                    aCAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                      <  3:48921/63‑1 (MQ=255)
                                                                                                            gCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATcgacga                                         <  4:152202/148‑1 (MQ=255)
                                                                                                            gCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGAt                                        >  2:6050/1‑149 (MQ=255)
                                                                                                             ccTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATg                                       >  3:200792/1‑149 (MQ=255)
                                                                                                              cTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGc                                      <  4:222854/149‑1 (MQ=255)
                                                                                                                gCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGt                                    >  4:322766/1‑149 (MQ=255)
                                                                                                                 cGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGt                                    <  4:200792/148‑1 (MQ=255)
                                                                                                                               gCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCg                     <  8:231871/149‑1 (MQ=255)
                                                                                                                               gCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCg                     <  2:131901/149‑1 (MQ=255)
                                                                                                                                 tCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGc                   >  3:222963/1‑149 (MQ=255)
                                                                                                                                  cACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTc                                                             >  4:115185/1‑106 (MQ=255)
                                                                                                                                  cACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTc                                                             <  3:115185/106‑1 (MQ=255)
                                                                                                                                   ttcGAGAAGCGCTTCAAGGCGCTGCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGa                 >  5:57794/3‑149 (MQ=255)
                                                                                                                                   ttcGAGAAGCGCTTCAAGGCGCTGCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGa                 >  1:84369/3‑149 (MQ=255)
                                                                                                                                     cGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATg               <  4:222963/149‑1 (MQ=255)
                                                                                                                                         cagcGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCg           >  8:288165/1‑149 (MQ=255)
                                                                                                                                             gcCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCtt       <  7:32672/149‑1 (MQ=255)
                                                                                                                                              ccTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTg      >  2:334467/1‑149 (MQ=255)
                                                                                                                                                   aaGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTGCCCa  <  4:223947/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTTCACCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTGCCCA  >  NC_002947/4518561‑4518855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑