Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 334,939 C→T 7.8% intergenic (‑254/+34) PP_0276 ← / ← PP_t73 Cro/CI family transcriptional regulator/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947334,9390CT 7.8% 146.1 / 2.9 51intergenic (‑254/+34)PP_0276/PP_t73Cro/CI family transcriptional regulator/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base T (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGTTT  >  NC_002947/334791‑335075
                                                                                                                                                    |                                                                                                                                        
gCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGcc                                                                                                                                          >  5:146415/1‑149 (MQ=255)
           gagaATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGt                                                                                                                               >  8:303159/1‑149 (MQ=255)
             gaATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGa                                                                                                                             <  2:209186/149‑1 (MQ=255)
                   cTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCATGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCttt                                                                                                                       <  6:223719/149‑1 (MQ=255)
                    ttCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCtttt                                                                                                                      >  7:220577/1‑149 (MQ=255)
                         cTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCTTTTTTgt                                                                                                                  <  8:220577/148‑2 (MQ=39)
                              ggtggtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGc                                                                                                            <  4:320540/149‑1 (MQ=255)
                                  gtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTAt                                                                                                        >  1:296166/1‑149 (MQ=255)
                                      gggCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGt                                                                                                     <  6:232554/148‑1 (MQ=255)
                                       ggCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGc                                                                                                   >  2:79487/1‑149 (MQ=255)
                                        gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCtttttt                                                                                                                    >  3:61592/1‑131 (MQ=39)
                                        gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCtttttt                                                                                                                    <  4:61592/131‑1 (MQ=39)
                                        gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGcc                                                                                                  <  8:122226/149‑1 (MQ=255)
                                        gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGcc                                                                                                  <  4:139551/149‑1 (MQ=255)
                                             tgcGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCAc                                                                                             >  6:246606/1‑149 (MQ=255)
                                               cgcAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCa                                                                                           <  2:324641/149‑1 (MQ=255)
                                                   gCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCatacg                                                                                       <  5:246606/149‑5 (MQ=255)
                                                    cccATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGGTTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAAt                                                                                      >  7:299328/1‑149 (MQ=255)
                                                           cAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACGAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAAccc                                                                               >  3:298143/1‑149 (MQ=255)
                                                           cAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAAccc                                                                               >  7:266570/1‑149 (MQ=255)
                                                            aGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCg                                                                              >  1:137064/1‑149 (MQ=255)
                                                              gCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCgg                                                                             >  6:132874/1‑148 (MQ=255)
                                                                aaGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGAc                                                                          >  2:14879/1‑149 (MQ=255)
                                                                aaGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGAc                                                                          <  8:266570/149‑1 (MQ=255)
                                                                  gCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGAcc                                                                         >  5:91082/1‑148 (MQ=255)
                                                                   ccAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTa                                                                       <  2:91052/149‑1 (MQ=255)
                                                                     aGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACt                                                                     >  7:294610/1‑149 (MQ=255)
                                                                            acacCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTAc                                                               >  4:116600/1‑148 (MQ=255)
                                                                            acacCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTAc                                                               >  1:336623/1‑148 (MQ=255)
                                                                             cacCGATCGTGGTGGATTCAGGGCATCCGTAAGTCAGGGATTGAGGGCTGCGGGGGCAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTAc                                                               <  1:99914/147‑1 (MQ=255)
                                                                             cacCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACaa                                                             <  5:132874/149‑1 (MQ=255)
                                                                                 gATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGt                                                           >  5:143525/1‑147 (MQ=255)
                                                                                 gATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCa                                                         <  8:46773/149‑1 (MQ=255)
                                                                                      tggtggATTCAGGGCATCCGTAAGGCATGGATTGTGGGCGGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGGTTACAAGTCAGTTGc                                                    <  2:173009/149‑1 (MQ=255)
                                                                                       ggtggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGc                                                    >  6:227555/1‑148 (MQ=255)
                                                                                        gtggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACGAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGct                                                   <  4:298143/148‑1 (MQ=255)
                                                                                         tggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACaa                                                             >  1:118076/1‑137 (MQ=255)
                                                                                         tggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACaa                                                             <  2:118076/137‑1 (MQ=255)
                                                                                           gATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTa                                                <  1:14879/148‑1 (MQ=255)
                                                                                           gATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTAc                                               >  8:218719/1‑149 (MQ=255)
                                                                                                 gggCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTg                                         <  6:91082/149‑1 (MQ=255)
                                                                                                            aGGCAGGGAGTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTAc                                               <  8:9795/132‑1 (MQ=255)
                                                                                                            aGGCAGGGACTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTAc                                               >  7:9795/1‑132 (MQ=255)
                                                                                                              gCAGGGATTGAGGGCTGCGGGGGTAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGc                             >  7:88915/1‑148 (MQ=255)
                                                                                                                   gATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATggg                       <  2:296166/149‑1 (MQ=255)
                                                                                                                   gATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATggg                       <  5:227555/149‑1 (MQ=255)
                                                                                                                        aGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGt                   >  3:181853/1‑148 (MQ=255)
                                                                                                                           gCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCATTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCAtt               <  7:303159/149‑1 (MQ=255)
                                                                                                                               cGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATtata            <  7:218719/148‑1 (MQ=255)
                                                                                                                                 gggggAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGc                     >  6:303255/1‑137 (MQ=255)
                                                                                                                                 gggggAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGc                     <  5:303255/137‑1 (MQ=255)
                                                                                                                                        aaCAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGttt  <  6:143525/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
GCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGTTT  >  NC_002947/334791‑335075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑