Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,518,715 T→G 6.7% K269Q (AAG→CAG)  clpA ← ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,518,7150TG 6.7% 188.1 / 4.3 60K269Q (AAG→CAG) clpAATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base T (24/32);  new base G (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

CCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTGCCCA  >  NC_002947/4518567‑4518855
                                                                                                                                                    |                                                                                                                                            
cccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCtt                                                                                                                                              <  6:68070/149‑1 (MQ=255)
 ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             >  3:174621/1‑149 (MQ=255)
 ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             >  3:241953/1‑149 (MQ=255)
 ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             >  1:150798/1‑149 (MQ=255)
 ccGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTtc                                                                                                                                             <  7:113908/149‑1 (MQ=255)
      gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                        <  3:311805/149‑1 (MQ=255)
      gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                        <  2:159084/149‑1 (MQ=255)
      gacAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGCATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGaa                                                                                                                                        >  3:86403/1‑149 (MQ=255)
        cagcagCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                      <  4:238330/149‑1 (MQ=255)
         agcagcGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGAAGCGCTTCAAGGCGCTGCTCggtgag                                                                                                                                     <  8:58673/149‑6 (MQ=255)
          gcagcGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGAAGCGCTTCAAGGCGCTGCTCggtgagc                                                                                                                                    <  8:162565/149‑7 (MQ=255)
           cagcGGCTTGAGCAGTTTGGAGGCATCCATCACGCCGCCCGACTCCGCACCGGCGCCGATGATGGTGTGTATTTCGTCGATGAACAGGATGTCCTGCGGACGCTTGCGCAGCTCACCGAGGAGCGCCTTGATGCGCTTCTCGAAGTcac                                                                                                                                   <  4:86403/149‑1 (MQ=255)
               ggCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACg                                                                                                                               <  7:244370/149‑1 (MQ=255)
                gCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACgg                                                                                                                              <  3:172041/149‑1 (MQ=255)
                      gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTAttt                                                                                                                         <  2:219452/148‑1 (MQ=255)
                      gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTg                                                                                                                        >  3:223947/1‑149 (MQ=255)
                      gCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTg                                                                                                                        <  3:221564/149‑1 (MQ=255)
                       cAGGTTGGAGGCATCCATCACCCCGCCAGTCGCCGCACCGGCGCCGATGTTGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                       <  4:13457/149‑1 (MQ=255)
                              gAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                                >  8:105077/1‑149 (MQ=255)
                               aGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                                >  6:306800/1‑148 (MQ=255)
                                ggCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGc                                                                                                                >  2:8337/1‑147 (MQ=255)
                                 gCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAg                                                                                                             <  1:138726/149‑1 (MQ=255)
                                   atccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGc                                                                                                            >  5:74359/1‑148 (MQ=255)
                                   atccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGc                                                                                                            >  2:285842/1‑148 (MQ=255)
                                    tccatcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                          <  5:12272/149‑1 (MQ=255)
                                      catcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAgg                                                                                                        <  4:268392/149‑1 (MQ=255)
                                       atcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                       >  3:240830/1‑133 (MQ=255)
                                       atcACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTgg                                                                                                                       <  4:240830/133‑1 (MQ=255)
                                               gcccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                          <  4:45456/138‑1 (MQ=255)
                                               gcccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCaa                                                                                                          >  3:45456/1‑138 (MQ=255)
                                                cccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGAt                                                                                               >  1:131901/1‑148 (MQ=255)
                                                cccgACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGCCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGAt                                                                                               >  1:112676/1‑148 (MQ=255)
                                                    aCGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAgg                                                                                          <  7:105077/149‑1 (MQ=255)
                                                    aCGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAgg                                                                                          <  4:241953/149‑1 (MQ=255)
                                                      gccgcACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGa                                                                                        <  1:285842/149‑1 (MQ=255)
                                                        cgcACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAg                                                                                       >  2:159808/1‑148 (MQ=255)
                                                          cACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTa                                                                                     >  8:32672/1‑148 (MQ=255)
                                                                        atgGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTgcgc                                                                      <  6:74359/149‑1 (MQ=255)
                                                                            tgtgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagc                                                                  <  2:150798/149‑1 (MQ=255)
                                                                              tgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagcag                                                                <  5:306800/149‑1 (MQ=255)
                                                                              tgAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCcagca                                                                 <  1:159808/148‑1 (MQ=255)
                                                                                  tttCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCa                                                            <  7:317168/149‑1 (MQ=255)
                                                                                     cgtcgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAgg                                                          >  1:304115/1‑148 (MQ=255)
                                                                                      gtcgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCa                                                        >  3:152202/1‑149 (MQ=255)
                                                                                        cgATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCAcc                                                      >  6:131261/1‑149 (MQ=255)
                                                                                              aCAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                      >  4:48921/1‑63 (MQ=255)
                                                                                              aCAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGt                                                                                                                                      <  3:48921/63‑1 (MQ=255)
                                                                                                      gCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATcgacga                                         <  4:152202/148‑1 (MQ=255)
                                                                                                      gCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGAt                                        >  2:6050/1‑149 (MQ=255)
                                                                                                       ccTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATg                                       >  3:200792/1‑149 (MQ=255)
                                                                                                        cTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGc                                      <  4:222854/149‑1 (MQ=255)
                                                                                                          gCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGt                                    >  4:322766/1‑149 (MQ=255)
                                                                                                           cGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGt                                    <  4:200792/148‑1 (MQ=255)
                                                                                                                         gCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCg                     <  2:131901/149‑1 (MQ=255)
                                                                                                                         gCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCg                     <  8:231871/149‑1 (MQ=255)
                                                                                                                           tCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGc                   >  3:222963/1‑149 (MQ=255)
                                                                                                                            cACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTc                                                             >  4:115185/1‑106 (MQ=255)
                                                                                                                            cACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTc                                                             <  3:115185/106‑1 (MQ=255)
                                                                                                                             ttcGAGAAGCGCTTCAAGGCGCTGCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGa                 >  1:84369/3‑149 (MQ=255)
                                                                                                                             ttcGAGAAGCGCTTCAAGGCGCTGCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGa                 >  5:57794/3‑149 (MQ=255)
                                                                                                                               cGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATg               <  4:222963/149‑1 (MQ=255)
                                                                                                                                   cagcGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCg           >  8:288165/1‑149 (MQ=255)
                                                                                                                                       gcCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCtt       <  7:32672/149‑1 (MQ=255)
                                                                                                                                        ccTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTg      >  2:334467/1‑149 (MQ=255)
                                                                                                                                             aaGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTGCCCa  <  4:223947/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CCCGACGACAGCAGCGGCTTGAGCAGGTTGGAGGCATCCATCACCCCGCCCGACGCCGCACCGGCGCCGATGATGGTGTGAATTTCGTCGATGAACAGGATGGCCTGCGGACGCTTGCGCAGCTCACCGAGCAGCGCCTTGAAGCGCTTCTCGAAGTCACCACGGTATTTGGTACCGGCCAGCAAGGCGCCCAGATCAAGGGAGTACACCACGCTCTGCGCCAGCAGGTCAGGCACCTGACCATCGACGATGCGTTTGGCCAGGCCTTCGGCGATGGCCGTCTTGCCCA  >  NC_002947/4518567‑4518855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑