Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,658,399 A→T 8.3% E345D (GAA→GAT) ‡ nuoC → NADH‑quinone oxidoreductase subunit C/D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,658,3990AT 8.3% 170.5 / 5.6 60E345D (GAA→GAT) ‡nuoCNADH‑quinone oxidoreductase subunit C/D
Reads supporting (aligned to +/- strand):  ref base A (26/29);  new base T (2/3);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

GGTCGACGTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGGCCG  >  NC_002947/4658252‑4658538
                                                                                                                                                   |                                                                                                                                           
ggTCGACGTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAg                                                                                                                                            >  5:184372/1‑149 (MQ=255)
    gACGTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGa                                                                                                                                         >  3:297397/1‑148 (MQ=255)
        tGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGCTTCGATCAcg                                                                                                                                    <  1:339403/149‑2 (MQ=255)
          aTTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCgg                                                                                                                                  >  7:38618/1‑149 (MQ=255)
            tCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGc                                                                                                                                 <  3:122293/148‑1 (MQ=255)
            tCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCt                                                                                                                                >  7:22528/1‑149 (MQ=255)
                       tGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCAc                                                                                                                     >  6:45499/1‑149 (MQ=255)
                           cGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGCCCCCGGTGTTATTAACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCgc                                                                                                                 >  6:284257/1‑148 (MQ=255)
                            gAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGc                                                                                                                <  2:37260/149‑1 (MQ=255)
                              gTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCt                                                                                                              >  4:327183/1‑149 (MQ=255)
                                  ttcCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTa                                                                                                          >  3:210595/1‑149 (MQ=255)
                                       cATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCa                                                                                                     >  3:49262/1‑149 (MQ=255)
                                             cAGCCACCTGCTGTTCCTTGGTACCTATATCCAGGACGTCGGCGCCATGAGCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATGACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTg                                                                                               <  6:30989/149‑1 (MQ=255)
                                               gCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGc                                                                                             <  1:146216/149‑1 (MQ=255)
                                               gCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGc                                                                                             <  6:184372/149‑1 (MQ=255)
                                               gCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCG‑CCGCCCGCGCGCCTTCACCCTTCTAGAAGCGGTACCCGGCTTACGCCTGCCCCCGGCCGGGTTCCGCCTCGGTTGCg                                                                                            >  1:176435/1‑149 (MQ=255)
                                                  aCCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCATGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTc                                                                                          >  4:129633/1‑149 (MQ=255)
                                                     tgctgTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGcc                                                                                       >  3:279758/1‑149 (MQ=255)
                                                     tgctgTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGcc                                                                                       >  6:120828/1‑149 (MQ=255)
                                                       ctgTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCa                                                                                     <  7:166809/149‑1 (MQ=255)
                                                             cTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCt                                                                               >  8:155291/1‑149 (MQ=255)
                                                                ggTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGcc                                                                            <  5:45499/149‑1 (MQ=255)
                                                                 gTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCa                                                                           >  1:275036/1‑149 (MQ=255)
                                                                   aCCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACg                                                                         >  5:74952/1‑149 (MQ=255)
                                                                     cTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGCTTCGATCAcg                                                                                                                                    >  3:28497/1‑87 (MQ=255)
                                                                     cTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGCTTCGATCAcg                                                                                                                                    <  4:28497/88‑2 (MQ=255)
                                                                     cTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTg                                                                       >  6:247268/1‑149 (MQ=255)
                                                                      tACATCCAGGAAGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTgg                                                                      >  6:162023/1‑149 (MQ=255)
                                                                       aCATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGc                                                                     >  3:294971/1‑149 (MQ=255)
                                                                           ccAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTgg                                                                  >  3:125649/1‑148 (MQ=255)
                                                                               gACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACaa                                                             <  8:38618/149‑1 (MQ=255)
                                                                                aCGTCGGCGCCATGACCCCGGTTTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAg                                                            <  6:303746/149‑1 (MQ=255)
                                                                                 cGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGc                                                           <  5:247268/149‑1 (MQ=255)
                                                                                   tCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTg                                                         <  6:74952/149‑1 (MQ=255)
                                                                                     ggCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTgg                                                        <  5:162023/148‑1 (MQ=255)
                                                                                     ggCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTgg                                                        <  4:126708/148‑1 (MQ=255)
                                                                                        gcCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTc                                                      >  8:278112/1‑147 (MQ=255)
                                                                                         ccATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAg                                                   <  4:210595/149‑1 (MQ=255)
                                                                                              aCCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGCTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACtt                                              <  2:200363/149‑1 (MQ=255)
                                                                                              aCCCCGGGGTTCTTCACATGC‑CCCTCCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtc                                             <  2:176435/149‑1 (MQ=255)
                                                                                               ccccGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtc                                             <  4:49262/149‑1 (MQ=255)
                                                                                                  cGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCATGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtcgtc                                          <  3:129633/149‑1 (MQ=255)
                                                                                                     tgtTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGaa                                       <  4:125649/149‑1 (MQ=255)
                                                                                                       ttcttcACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAAt                                      <  2:275036/148‑1 (MQ=255)
                                                                                                         cttcaccttcacCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATgg                                    <  4:297397/148‑1 (MQ=255)
                                                                                                         cttcaccttcacCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGc                                   >  3:249070/1‑149 (MQ=255)
                                                                                                             accttcaccGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGc                                <  4:294971/148‑1 (MQ=255)
                                                                                                                  caccGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCaa                            <  7:191895/147‑1 (MQ=255)
                                                                                                                   accgaccgCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAgc                          <  4:249070/148‑1 (MQ=255)
                                                                                                                          gCCAGCGCGCATACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTcgac                  >  2:60948/1‑149 (MQ=255)
                                                                                                                            cAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTcgacg                 >  7:248224/1‑148 (MQ=255)
                                                                                                                             aGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGaa               <  7:278112/149‑1 (MQ=255)
                                                                                                                              gcgcgcCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAAt              <  6:260504/149‑1 (MQ=255)
                                                                                                                                 cgcCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATaca           <  7:155291/149‑1 (MQ=255)
                                                                                                                                       ctccGTGATCGAAGCGATCAGCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAg      <  3:183950/146‑1 (MQ=255)
                                                                                                                                         cggTGATCGCTTCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtcgtc                                          >  6:109200/3‑110 (MQ=255)
                                                                                                                                         cggTGATCGCTTCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtcgtc                                          <  5:109200/108‑1 (MQ=255)
                                                                                                                                          cGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATa             <  1:217120/138‑1 (MQ=255)
                                                                                                                                          cGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATa             >  2:217120/1‑138 (MQ=255)
                                                                                                                                          cGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGgccg  >  2:248814/1‑149 (MQ=255)
                                                                                                                                                  aaGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtcg                                            >  3:305098/1‑99 (MQ=255)
                                                                                                                                                  aaGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtcg                                            <  4:305098/99‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GGTCGACGTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGGCCG  >  NC_002947/4658252‑4658538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑