Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 334,952 A→G 7.5% intergenic (‑267/+21) PP_0276 ← / ← PP_t73 Cro/CI family transcriptional regulator/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947334,9520AG 7.5% 149.7 / 2.5 53intergenic (‑267/+21)PP_0276/PP_t73Cro/CI family transcriptional regulator/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base G (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGTTTGGCGCCTGTAAACCACTTCCTT  >  NC_002947/334804‑335097
                                                                                                                                                    |                                                                                                                                                 
gaATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGa                                                                                                                                                   <  2:209186/149‑1 (MQ=255)
      cTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCATGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCttt                                                                                                                                             <  6:223719/149‑1 (MQ=255)
       ttCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCtttt                                                                                                                                            >  7:220577/1‑149 (MQ=255)
            cTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCTTTTTTgt                                                                                                                                        <  8:220577/148‑2 (MQ=39)
                 ggtggtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGc                                                                                                                                  <  4:320540/149‑1 (MQ=255)
                     gtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTAt                                                                                                                              >  1:296166/1‑149 (MQ=255)
                         gggCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGt                                                                                                                           <  6:232554/148‑1 (MQ=255)
                          ggCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGc                                                                                                                         >  2:79487/1‑149 (MQ=255)
                           gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCtttttt                                                                                                                                          <  4:61592/131‑1 (MQ=39)
                           gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCTCACCGAAAGGTGGGCCtttttt                                                                                                                                          >  3:61592/1‑131 (MQ=39)
                           gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGcc                                                                                                                        <  8:122226/149‑1 (MQ=255)
                           gctgctGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGcc                                                                                                                        <  4:139551/149‑1 (MQ=255)
                                tgcGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCAc                                                                                                                   >  6:246606/1‑149 (MQ=255)
                                  cgcAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCa                                                                                                                 <  2:324641/149‑1 (MQ=255)
                                      gCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCatacg                                                                                                             <  5:246606/149‑5 (MQ=255)
                                       cccATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGGTTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAAt                                                                                                            >  7:299328/1‑149 (MQ=255)
                                              cAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACGAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAAccc                                                                                                     >  3:298143/1‑149 (MQ=255)
                                              cAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAAccc                                                                                                     >  7:266570/1‑149 (MQ=255)
                                               aGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCg                                                                                                    >  1:137064/1‑149 (MQ=255)
                                                 gCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCgg                                                                                                   >  6:132874/1‑148 (MQ=255)
                                                   aaGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGAc                                                                                                <  8:266570/149‑1 (MQ=255)
                                                   aaGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGAc                                                                                                >  2:14879/1‑149 (MQ=255)
                                                     gCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGAcc                                                                                               >  5:91082/1‑148 (MQ=255)
                                                      ccAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTa                                                                                             <  2:91052/149‑1 (MQ=255)
                                                        aGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACt                                                                                           >  7:294610/1‑149 (MQ=255)
                                                               acacCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTAc                                                                                     >  4:116600/1‑148 (MQ=255)
                                                               acacCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTAc                                                                                     >  1:336623/1‑148 (MQ=255)
                                                                cacCGATCGTGGTGGATTCAGGGCATCCGTAAGTCAGGGATTGAGGGCTGCGGGGGCAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTAc                                                                                     <  1:99914/147‑1 (MQ=255)
                                                                cacCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACaa                                                                                   <  5:132874/149‑1 (MQ=255)
                                                                    gATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGt                                                                                 >  5:143525/1‑147 (MQ=255)
                                                                    gATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCa                                                                               <  8:46773/149‑1 (MQ=255)
                                                                         tggtggATTCAGGGCATCCGTAAGGCATGGATTGTGGGCGGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGGTTACAAGTCAGTTGc                                                                          <  2:173009/149‑1 (MQ=255)
                                                                          ggtggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGc                                                                          >  6:227555/1‑148 (MQ=255)
                                                                           gtggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACGAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGct                                                                         <  4:298143/148‑1 (MQ=255)
                                                                            tggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACaa                                                                                   >  1:118076/1‑137 (MQ=255)
                                                                            tggATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACaa                                                                                   <  2:118076/137‑1 (MQ=255)
                                                                              gATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTa                                                                      <  1:14879/148‑1 (MQ=255)
                                                                              gATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTAc                                                                     >  8:218719/1‑149 (MQ=255)
                                                                                    gggCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTg                                                               <  6:91082/149‑1 (MQ=255)
                                                                                               aGGCAGGGAGTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTAc                                                                     <  8:9795/132‑1 (MQ=255)
                                                                                               aGGCAGGGACTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTAc                                                                     >  7:9795/1‑132 (MQ=255)
                                                                                                 gCAGGGATTGAGGGCTGCGGGGGTAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGc                                                   >  7:88915/1‑148 (MQ=255)
                                                                                                      gATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATggg                                             <  5:227555/149‑1 (MQ=255)
                                                                                                      gATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATggg                                             <  2:296166/149‑1 (MQ=255)
                                                                                                           aGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGt                                         >  3:181853/1‑148 (MQ=255)
                                                                                                              gCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCATTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCAtt                                     <  7:303159/149‑1 (MQ=255)
                                                                                                                  cGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATtata                                  <  7:218719/148‑1 (MQ=255)
                                                                                                                    gggggAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGc                                           >  6:303255/1‑137 (MQ=255)
                                                                                                                    gggggAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGc                                           <  5:303255/137‑1 (MQ=255)
                                                                                                                           aaCAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGttt                        <  6:143525/149‑1 (MQ=255)
                                                                                                                                         aCCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGTTTGGCGCCTGTAAAc           >  7:34830/1‑148 (MQ=255)
                                                                                                                                           cTTTCGGTGAGCCATTATTGATACAGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGTCGTCATTATAGCGATCGTTTGGCGCCTGTAAACCAc        >  7:282040/1‑149 (MQ=255)
                                                                                                                                                   gAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGTTTGGCGCCTGTAAACCACTTCCtt  <  6:289934/147‑1 (MQ=255)
                                                                                                                                                   gAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGGCATTATAGCGATCGTTTGGCGCCTGTAAACCACTTCCtt  >  5:289934/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCACCAGGAATCGAACCCGGGACCTACTGATTACAAGTCAGTTGCTCTACCATCTGAGCTATACCGGCAATGGGGCGTCATTATAGCGATCGTTTGGCGCCTGTAAACCACTTCCTT  >  NC_002947/334804‑335097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑