Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,790,319 T→A 7.5% E1174V (GAG→GTG)  pvdI ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,790,3190TA 7.5% 155.9 / 3.1 53E1174V (GAG→GTG) pvdInon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CGAGCAGTAGGTGCTCCTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCTTGGGCCAGCGCTTCACGGTCCAGGGCACCGGT  >  NC_002947/4790176‑4790458
                                                                                                                                               |                                                                                                                                           
cGAGCAGTAGGTGCTCCTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAgg                                                                                                                                        <  8:78313/149‑1 (MQ=255)
      gTAGGTGCTCCTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGAcc                                                                                                                                  >  3:100789/1‑149 (MQ=255)
       tAGGTGCTCCTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCACAAGGGTGACcg                                                                                                                                 >  5:240983/1‑149 (MQ=255)
       tAGGTGCTCCTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCACAAGGGTGACcg                                                                                                                                 >  3:213351/1‑149 (MQ=255)
             cTCCTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCgaga                                                                                                                            <  7:98848/148‑1 (MQ=255)
                cTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGAtt                                                                                                                          <  1:139178/147‑1 (MQ=255)
                  gCTCGCCACGCTGATGCAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGt                                                                                                                                      >  5:243750/1‑133 (MQ=255)
                  gCTCGCCACGCTGATGCAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGt                                                                                                                                      <  6:243750/133‑1 (MQ=255)
                   cTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGt                                                                                                                     >  5:270903/1‑149 (MQ=255)
                      gCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTg                                                                                                                    <  4:230958/147‑1 (MQ=255)
                      gCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGa                                                                                                                  >  3:67793/1‑149 (MQ=255)
                       ccACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGAc                                                                                                                 >  3:118291/1‑149 (MQ=255)
                       ccACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGAc                                                                                                                 >  8:125249/1‑149 (MQ=255)
                        cACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGt                                                                                                                     <  4:156429/144‑1 (MQ=255)
                        cACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGt                                                                                                                     >  3:156429/1‑144 (MQ=255)
                                   aGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAg                                                                                                                                         >  8:309278/1‑113 (MQ=255)
                                   aGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGc                                                                                                                       <  8:225043/131‑1 (MQ=255)
                                   aGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGc                                                                                                                       >  7:225043/1‑131 (MQ=255)
                                     cTTCATTCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTTGATGGCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTGAAATGGTTGCGCCTGCTGCTTCTCAAGGGTGACCGAGAGATTGTAGTGGACTCGCAAGCCTGGca                                                                                                   <  6:89286/149‑1 (MQ=255)
                                         aTGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCacac                                                                                                <  4:118291/148‑1 (MQ=255)
                                          tGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCACAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCt                                                                                              <  4:213351/149‑1 (MQ=255)
                                          tGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCACAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCt                                                                                              <  6:240983/149‑1 (MQ=255)
                                               aTCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTtgctg                                                                                         <  4:326074/149‑1 (MQ=255)
                                                         cGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGaaa                                                                               <  4:100789/149‑1 (MQ=255)
                                                          gTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAAc                                                                              <  8:236872/149‑1 (MQ=255)
                                                             ggCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAg                                                                            <  6:270903/148‑1 (MQ=255)
                                                             ggCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAg                                                                            <  1:149063/148‑1 (MQ=255)
                                                              gCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAgg                                                                           >  2:304612/1‑148 (MQ=255)
                                                               cAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAgg                                                                                                     <  8:237505/121‑1 (MQ=255)
                                                                 aGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACg                                                                       <  7:125249/149‑1 (MQ=255)
                                                                   gTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGc                                                                      >  4:221724/1‑148 (MQ=255)
                                                                      gAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGa                                                                                 >  5:5158/1‑134 (MQ=255)
                                                                      gAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGa                                                                                 <  6:5158/134‑1 (MQ=255)
                                                                      gAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAAt                                                                   >  5:68615/1‑148 (MQ=255)
                                                                                  gATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGt                                                       <  3:221724/148‑1 (MQ=255)
                                                                                     gTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATg                                                                  <  4:237306/134‑1 (MQ=255)
                                                                                     gTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATg                                                                  >  3:237306/1‑134 (MQ=255)
                                                                                             gCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGcc                                           >  1:3927/1‑149 (MQ=255)
                                                                                                     tgcAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGc                                   >  5:262951/1‑149 (MQ=255)
                                                                                                      gcAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGc                                                                                                                       >  5:319822/1‑64 (MQ=255)
                                                                                                      gcAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGc                                                                                                                       <  6:319822/64‑1 (MQ=255)
                                                                                                      gcAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAGACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCt                                  >  2:261196/1‑149 (MQ=255)
                                                                                                      gcAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCt                                  >  7:163596/1‑149 (MQ=255)
                                                                                                      gcAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCt                                  >  7:171098/1‑149 (MQ=255)
                                                                                                       cAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGc                                   <  6:68615/147‑1 (MQ=255)
                                                                                                       cAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCtt                                 <  4:67793/149‑1 (MQ=255)
                                                                                                       cAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCtt                                 >  5:196721/1‑149 (MQ=255)
                                                                                                         aCAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCTTgg                               >  4:268192/1‑149 (MQ=255)
                                                                                                             ttGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCTTGGGc                             >  3:255451/1‑147 (MQ=255)
                                                                                                                     ggTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCTTGGGCCAGCGCTTCa                   <  8:171098/149‑1 (MQ=255)
                                                                                                                          aaGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTc                                                              <  7:241223/101‑1 (MQ=255)
                                                                                                                          aaGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTc                                                              >  8:241223/1‑101 (MQ=255)
                                                                                                                              cTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCTTGGGCCAGCGCTTCACGGTCCAgg          <  2:3927/149‑1 (MQ=255)
                                                                                                                                        ctgctgctCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCTTGGGCCAGCGCTTCACGGTCCAGGGCACCGGt  <  8:163596/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                           
CGAGCAGTAGGTGCTCCTGCTCGCCACGCTGATACAGCTTCATGCGAATCAGCGGCCCGTTGGCAAGGTCGAATGGCTGCTGGATGTCGGCCTGCAACTGCTGCAACAGTTGCGCGGGGTCAAAGGCTTGCGCCTGCTGCTGCTCAAGGGTGACCGCGAGATTGGCGTGGACGCGCAAGCCAGGCACACCTTGCTGCTCTTCGAAACAGGTACGCAATGATTCATGCCGTGACACCAGCGCCGAAAACGCTTGGGCCAGCGCTTCACGGTCCAGGGCACCGGT  >  NC_002947/4790176‑4790458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑