Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,818,565 C→G 7.5% D4209H (GAC→CAC)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,818,5650CG 7.5% 164.3 / 4.7 53D4209H (GAC→CAC) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (26/23);  new base G (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GCCAGCAATGAATGCCCGCCCAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAACGCGGCATCGG  >  NC_002947/4818417‑4818708
                                                                                                                                                    |                                                                                                                                               
gCCAGCAATGAATGCCCGCCCAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTc                                                                                                                                                 <  8:26470/149‑1 (MQ=255)
  cAGCAATGAATGCCCGCCCAGTTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGa                                                                                                                                               >  6:294762/1‑149 (MQ=255)
  cAGCAATGAATGCCCGCCCAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGa                                                                                                                                               >  6:257239/1‑149 (MQ=255)
  cAGCAATGAATGCCCGCCCAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGa                                                                                                                                               >  3:159330/1‑149 (MQ=255)
  cAGCAATGAATGCCCGCCCAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGa                                                                                                                                               >  3:154242/1‑149 (MQ=255)
              cccgcccAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAgg                                                                                                                                    <  7:43592/148‑1 (MQ=255)
               ccgcccAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAg                                                                                                                                     >  8:258234/1‑146 (MQ=255)
               ccgcccAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAg                                                                                                                                     <  7:258234/146‑1 (MQ=255)
                  cccAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCtt                                                                                                                               <  6:276412/149‑1 (MQ=255)
                                gTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGc                                                                                                                 >  3:179817/1‑149 (MQ=255)
                                 ttGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGcc                                                                                                                <  4:159330/149‑1 (MQ=255)
                                 ttGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGcc                                                                                                                <  4:154242/149‑1 (MQ=255)
                                  tGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCg                                                                                                               >  3:219402/1‑149 (MQ=255)
                                   gTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGt                                                                                                              >  7:251206/1‑149 (MQ=255)
                                    tCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGtt                                                                                                             >  8:292868/1‑149 (MQ=255)
                                          aCTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGtt                                                                                                       >  1:225037/1‑149 (MQ=255)
                                            tCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTtgt                                                                                                     >  3:210676/1‑149 (MQ=255)
                                              ccACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGc                                                                                                   <  4:179817/149‑1 (MQ=255)
                                              ccACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGc                                                                                                   <  2:254425/149‑1 (MQ=255)
                                               cACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCg                                                                                                  <  4:219402/149‑1 (MQ=255)
                                                        ggCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGt                                                                                         >  7:254806/1‑149 (MQ=255)
                                                              gAGCACATCGGCCCAAATCCCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGGCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAAc                                                                                    <  2:240805/148‑1 (MQ=255)
                                                                  acaTCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGcc                                                                               <  5:257239/149‑1 (MQ=255)
                                                                    aacGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGccc                                                                              >  4:254237/3‑148 (MQ=255)
                                                                          ccAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGAtggc                                                                                                                                           >  4:310585/1‑81 (MQ=255)
                                                                          ccAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGAtggc                                                                                                                                           <  3:310585/81‑1 (MQ=255)
                                                                          ccAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGc                                                                        >  2:317267/1‑148 (MQ=255)
                                                                                       aaCACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTa                                                          <  7:292868/149‑1 (MQ=255)
                                                                                          acGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCg                                                                                                  <  2:177072/106‑1 (MQ=255)
                                                                                          acGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCg                                                                                                  >  1:177072/1‑106 (MQ=255)
                                                                                            gTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCg                                                     <  7:88038/149‑1 (MQ=255)
                                                                                            gTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCg                                                     >  8:88038/1‑149 (MQ=255)
                                                                                              cctccAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGt                                                                          >  5:181287/1‑126 (MQ=255)
                                                                                              cctccAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGt                                                                          <  6:181287/126‑1 (MQ=255)
                                                                                                tccAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCaa                                                                      <  3:119317/128‑1 (MQ=255)
                                                                                                tccAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCaa                                                                      >  4:119317/1‑128 (MQ=255)
                                                                                                  cAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCAAGTACTCCGGAAACt                                               >  1:216135/1‑149 (MQ=255)
                                                                                                       cGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGc                                           <  8:254806/148‑1 (MQ=255)
                                                                                                           gCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACg                                        >  4:222233/1‑147 (MQ=255)
                                                                                                             gTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGt                                    >  8:201213/1‑149 (MQ=255)
                                                                                                              tGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTGGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTc                                   >  4:131203/1‑149 (MQ=255)
                                                                                                              tGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTGGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTc                                   >  4:136184/1‑149 (MQ=255)
                                                                                                                   ccAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGc                              >  5:329884/1‑149 (MQ=255)
                                                                                                                     aGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCtt                            <  2:225037/149‑1 (MQ=255)
                                                                                                                                aCTGTAGTGACCGCCGATGTCTATGGGTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTcc                  <  7:201213/148‑1 (MQ=255)
                                                                                                                                 cTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAg                <  6:329884/149‑1 (MQ=255)
                                                                                                                                         aCCGCCGATGTGGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAAcgcg         <  3:136184/148‑1 (MQ=255)
                                                                                                                                         aCCGCCGATGTGGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAAcgcg         <  3:131203/148‑1 (MQ=255)
                                                                                                                                         aCCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAAcgc          <  5:294762/147‑1 (MQ=255)
                                                                                                                                         aCCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAACGCgg        >  1:6651/1‑149 (MQ=255)
                                                                                                                                         aCCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTGGAGCAGGTCAAGCAAcga          <  3:254237/147‑2 (MQ=255)
                                                                                                                                           cgccgATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAACGCGGCa      >  2:238216/1‑149 (MQ=255)
                                                                                                                                           cgccgATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAACGCGGCa      >  2:128650/1‑149 (MQ=255)
                                                                                                                                               gATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAACGCGGCATCgg  <  1:317267/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCCAGCAATGAATGCCCGCCCAGCTCGAAGAAGTTGTCGTGCACTCCCACCCGCTCGGCTTTGAGCACATCGGCCCAAATACCCGCCAACACGTCCTCCAATTCGTTGCGTGGCGCCAGATAGGCCTGACTGTACTGACCGCCGATGTCGATGGCTGGCAGGGCCTTGCGGTCGAGCTTGCCGTTGGCGTTGTGCGGCAAGCTGTCGAACCAGCCCCAGTGCAAGGGCACCATGTACTCCGGCAACTCGGCACGCAGTCGTTGCTTGACCTGCTCCAGCAACGCGGCATCGG  >  NC_002947/4818417‑4818708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑