Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,986,048 C→G 8.9% P97R (CCG→CGG)  flgA → flagella basal body P‑ring formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,986,0480CG 8.9% 162.7 / 7.4 56P97R (CCG→CGG) flgAflagella basal body P‑ring formation protein
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base G (3/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

TTTCTTGAATTCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTCGGCGAAGGT  >  NC_002947/4985903‑4986187
                                                                                                                                                 |                                                                                                                                           
tttCTTGAATTCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCt                                                                                                                                          >  7:147515/1‑149 (MQ=255)
tttCTTGAATTCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCACGGCCCAGCGGCt                                                                                                                                          >  6:23312/1‑149 (MQ=255)
   cTTGAATTCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTggg                                                                                                                                       >  1:274529/1‑149 (MQ=255)
      gAATTCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTggg                                                                                                                                       <  5:86407/146‑1 (MQ=255)
        aTTCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCTTTGTGCAGCCAGCAGCTGGACGCCTCACAGGAAAGCCCGGCCCAGCCGCTGGGCCgt                                                                                                                                   <  1:192442/148‑1 (MQ=255)
         ttCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCGGCTGGGCCggg                                                                                                                                  <  8:306790/148‑3 (MQ=255)
         ttCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCGGCTGGGCCggg                                                                                                                                  >  7:306790/1‑146 (MQ=255)
            aCCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGAc                                                                                                                              <  8:147515/149‑1 (MQ=255)
            aCCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGATGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGAc                                                                                                                              >  5:261385/1‑149 (MQ=255)
             ccGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCACGGCCCAGCGGCTGGGCCgggctttc                                                                                                                             <  5:23312/149‑8 (MQ=255)
               gTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGt                                                                                                                           <  2:331942/149‑1 (MQ=255)
                ttGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTa                                                                                                                          >  8:194390/1‑149 (MQ=255)
                          tCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCt                                                                                                                >  1:149393/1‑149 (MQ=255)
                                  ccaccCAGACTGCCGGGCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGgc                                                                                                        <  7:288660/149‑1 (MQ=255)
                                     cccAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGt                                                                                                                           >  8:221559/1‑127 (MQ=255)
                                     cccAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGt                                                                                                                           <  7:221559/127‑1 (MQ=255)
                                                     cTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACg                                                                                         >  3:217971/1‑145 (MQ=255)
                                                     cTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCAGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACg                                                                                         <  4:217971/145‑1 (MQ=255)
                                                          aaaTCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGt                                                                                 <  8:290017/148‑1 (MQ=255)
                                                           aaTCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGc                                                                               >  5:241193/1‑149 (MQ=255)
                                                            aTCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGcc                                                                              >  2:83150/1‑149 (MQ=255)
                                                             tCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCg                                                                             >  4:110887/1‑149 (MQ=255)
                                                               cAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCgg                                                                            <  2:321005/148‑1 (MQ=255)
                                                                     aaCCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACgg                                                                                                                       <  1:122876/99‑1 (MQ=255)
                                                                     aaCCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACgg                                                                                                                       >  2:122876/1‑99 (MQ=255)
                                                                     aaCCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGt                                                                     <  4:90020/149‑1 (MQ=255)
                                                                      aCCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAGGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTg                                                                    >  3:150885/1‑149 (MQ=255)
                                                                       cccGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGc                                                                   >  4:305107/1‑149 (MQ=255)
                                                                         cGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGa                                                                 <  3:110887/149‑1 (MQ=255)
                                                                              gACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGt                                                                                              >  4:262040/1‑115 (MQ=255)
                                                                              gACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGt                                                                                              <  3:262040/115‑1 (MQ=255)
                                                                              gACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACtttt                                                            <  6:273063/149‑1 (MQ=255)
                                                                               aCCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAACGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTc                                                           <  7:142351/149‑1 (MQ=255)
                                                                                cccACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTcc                                                          <  3:305107/149‑1 (MQ=255)
                                                                                 ccACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCcg                                                         >  1:108199/1‑149 (MQ=255)
                                                                                   aCGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCcg                                                         >  8:20356/1‑147 (MQ=255)
                                                                                   aCGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCcg                                                         <  7:20356/147‑1 (MQ=255)
                                                                                         gcgcATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCCCCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTGTTCCGCGACgtgg                                                 >  2:255564/1‑149 (MQ=255)
                                                                                              tGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAGGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACgtggtggtg                                            <  4:150885/149‑1 (MQ=255)
                                                                                              tGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACgtggtggtg                                            <  6:308210/149‑1 (MQ=255)
                                                                                                ccGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACgtggtggtgg                                           <  7:194390/148‑1 (MQ=255)
                                                                                                        cagccagcAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGAcccgcc                                  >  3:244492/1‑149 (MQ=255)
                                                                                                        cagccagcAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGAcccgcc                                  >  6:117713/1‑149 (MQ=255)
                                                                                                        cagccagcAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCGGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGAcccgcc                                  >  6:139563/1‑149 (MQ=255)
                                                                                                           ccagcAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACgtggtggtgg                                           >  5:254382/1‑137 (MQ=255)
                                                                                                           ccagcAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACgtggtggtgg                                           <  6:254382/137‑1 (MQ=255)
                                                                                                                         ccTCACTGGAAAGCACGGCCCAGCCGCTGGGCCGTGTGACTGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCAGCCCGATCAAGCGCGacaac                 <  6:261385/149‑1 (MQ=255)
                                                                                                                             aCTGGAAGGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACgg             >  7:180176/1‑149 (MQ=255)
                                                                                                                               tGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGacaac                 >  7:236120/1‑143 (MQ=255)
                                                                                                                               tGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGacaac                 <  8:236120/143‑1 (MQ=255)
                                                                                                                               tGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTc           >  7:244874/1‑149 (MQ=255)
                                                                                                                                 gAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTc           <  2:149393/147‑1 (MQ=255)
                                                                                                                                    cacacGGCCCAGCGGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTCGGCg       >  2:45451/5‑148 (MQ=255)
                                                                                                                                     gCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTCGGCGaa     <  8:244874/149‑1 (MQ=255)
                                                                                                                                      cccGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTCGGCGAAg    <  8:180176/149‑1 (MQ=255)
                                                                                                                                         ggCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTCGGCGAAGGt  >  4:311290/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
TTTCTTGAATTCACCGTTGAAGATTATCTGTCCACCACCCAGACTGCCGGCCGCTATGAAATCCAGGTCAACCCGCTGGACCCACGCCTGCGCATGCCGTTGTGCAGCCAGCAGCTGGACGCCTCACTGGAAAGCCCGGCCCAGCCGCTGGGCCGTGTGACGGTACGGATACGCTGCAACGGCGCAGCACCGTGGACGGTATTCGTGCCGGCCACCGTGCGACTTTTCCGCGACGTGGTGGTGGTGACCCGCCCGCTCAAGCGCGACAACACGGTCGGCGAAGGT  >  NC_002947/4985903‑4986187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑