Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,107,492 C→G 7.8% G207A (GGC→GCC)  ydiJ ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,107,4920CG 7.8% 199.0 / 6.4 64G207A (GGC→GCC) ydiJputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base G (2/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

TTGAGGTTGAAGCGGCCCTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCATCCG  >  NC_002947/5107345‑5107636
                                                                                                                                                   |                                                                                                                                                 
ttGAGGTTGAAGCGGACCTGCTCGTCGCGCAGGTGCGCCAGGTATTAGCCGGTCAGGCAACGGTTGTGCTGGGGGAAGGTTGTTTCAATAAGTTCTGCCTGGGTTTCCTTAATTTCCCGGGCAATGCGGTACACCTCGCAGAcccggcc                                                                                                                                                  <  2:98974/149‑1 (MQ=255)
        gAAGCGGCCCTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCcggcgcgg                                                                                                                                          >  6:186570/1‑149 (MQ=255)
        ctaccgtcccTGCTCGTCGCCCAGGAGCGCCAGGTCGAAGCCGGTCCGGCAACGGTTGAGCTTGGGGAAGCTGGTTTCAAACAGTTCGGCCTCGGGTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCcggcgcgg                                                                                                                                          <  7:284144/142‑1 (MQ=255)
           gCGGCCCTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACCCATCGCCGCACCGGCCcggcgcgg                                                                                                                                          >  8:284144/1‑146 (MQ=255)
           gCGGCCCTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGCGCCGGGCcgggtcggcga                                                                                                                                       <  7:198785/149‑2 (MQ=255)
            cGGCCCTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCa                                                                                                                                      <  6:112156/149‑1 (MQ=255)
            cGGCCCTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCa                                                                                                                                      <  7:301349/149‑1 (MQ=255)
               cccTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGc                                                                                                                                   <  4:259202/149‑1 (MQ=255)
                 cTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGc                                                                                                                                   >  8:44785/1‑147 (MQ=255)
                 cTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGc                                                                                                                                   <  7:44785/147‑1 (MQ=255)
                                gTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCAt                                                                                                                  >  2:31366/1‑149 (MQ=255)
                                   cgcCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCATTCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCAtcgt                                                                                                               <  7:23101/149‑1 (MQ=255)
                                      cAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGtt                                                                                                                              >  6:242754/1‑131 (MQ=255)
                                      cAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGa                                                                                                            >  5:228061/1‑149 (MQ=255)
                                        ggTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATc                                                                                                          <  1:38944/149‑1 (MQ=255)
                                           cGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCgg                                                                                                        >  8:243848/1‑148 (MQ=255)
                                            gTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCgg                                                                                                        >  2:320556/1‑147 (MQ=255)
                                            gTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCa                                                                                                      <  7:121176/149‑1 (MQ=255)
                                            gTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCa                                                                                                      <  7:203993/149‑1 (MQ=255)
                                             tAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAg                                                                                                     >  5:35785/1‑149 (MQ=255)
                                               gCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGc                                                                                                   <  1:203050/149‑1 (MQ=255)
                                                 cGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCtg                                                                                                 <  4:161458/149‑1 (MQ=255)
                                                   gTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCtgtg                                                                                               >  7:310593/1‑149 (MQ=255)
                                                      aGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAg                                                                                            <  7:243848/149‑1 (MQ=255)
                                                      aGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAg                                                                                            <  1:320556/149‑1 (MQ=255)
                                                               ttGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGcc                                                                                   <  7:131792/149‑1 (MQ=255)
                                                                 gAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCa                                                                                  <  8:310593/148‑1 (MQ=255)
                                                                  aGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGc                                                                                              >  2:49517/1‑135 (MQ=255)
                                                                  aGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGc                                                                                              <  1:49517/135‑1 (MQ=255)
                                                                  aGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCAc                                                                                 <  2:82237/148‑1 (MQ=255)
                                                                   gCTTGGGGAAGGTTGTTTCAATCAGTTCGGCCTGGGTTTCCTTAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCAcc                                                                                <  7:281326/148‑1 (MQ=255)
                                                                              gtggtTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCt                                                                    >  2:326517/1‑149 (MQ=255)
                                                                                ggtTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCtgt                                                                  >  6:164706/1‑149 (MQ=255)
                                                                                  tttCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCAGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCtgtg                                                                 >  7:136775/1‑148 (MQ=255)
                                                                                          aGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAg                                                        <  5:186570/149‑1 (MQ=255)
                                                                                           gTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAg                                                        >  8:146920/1‑148 (MQ=255)
                                                                                           gTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAg                                                        >  3:35586/1‑148 (MQ=255)
                                                                                                gCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTg                                                  >  4:19526/1‑149 (MQ=255)
                                                                                                      gTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTcgcg                                            >  3:138532/1‑149 (MQ=255)
                                                                                                           cTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  8:10857/1‑149 (MQ=255)
                                                                                                           cTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  5:166540/1‑149 (MQ=255)
                                                                                                           cTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  4:266417/1‑149 (MQ=255)
                                                                                                           cTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  3:220734/1‑149 (MQ=255)
                                                                                                            tGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  3:273362/1‑148 (MQ=255)
                                                                                                            tGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  8:43927/1‑148 (MQ=255)
                                                                                                            tGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  2:148077/1‑148 (MQ=255)
                                                                                                            tGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGAGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCAACGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑ttt                                      >  7:116820/1‑148 (MQ=255)
                                                                                                              aaTTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTAc                                   >  2:331365/1‑149 (MQ=255)
                                                                                                                   cccGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTa                               <  5:164706/148‑1 (MQ=255)
                                                                                                                    ccGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCCGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCCCCGAGCAGCACGCTGTGCAGCTCCAGCACGGGGTCGCGGG‑TTTTACCGTAgg                             >  2:52781/1‑149 (MQ=255)
                                                                                                                    ccGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAg                              <  6:35785/148‑1 (MQ=255)
                                                                                                                          cATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGCTCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGc                       <  8:138466/149‑1 (MQ=255)
                                                                                                                              cGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGcc                   <  7:10857/149‑1 (MQ=255)
                                                                                                                              cGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGc                    <  4:220734/148‑1 (MQ=255)
                                                                                                                                   ggctgcgccgcgccgGGCCGGGTCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCGGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTGGc              >  5:340505/6‑149 (MQ=255)
                                                                                                                                   gcctgcgccgcgccgGGCCGGGTCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTgg               <  6:340505/143‑1 (MQ=255)
                                                                                                                                    acCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTGGcc             <  1:326517/149‑1 (MQ=255)
                                                                                                                                     ctgcgccgcgccgGGCCGGGTCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTg                <  1:254102/142‑1 (MQ=255)
                                                                                                                                     ctgcgccgcgccgGGCCGGGTCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTg                >  2:254102/4‑145 (MQ=255)
                                                                                                                                     ccTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTGGCCa            <  3:266417/149‑1 (MQ=255)
                                                                                                                                      cTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTGGCCAc           >  2:236319/1‑149 (MQ=255)
                                                                                                                                        cGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAg                                                              >  6:262199/1‑97 (MQ=255)
                                                                                                                                        cGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAg                                                              <  5:262199/97‑1 (MQ=255)
                                                                                                                                        cGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TGTTACCGTAGGTGCAGCTGCCCTGGCCACTg         >  8:231432/1‑149 (MQ=255)
                                                                                                                                         gCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTTACCGTAGGTGCAGCTGCCCTGGCCACt          <  1:31366/148‑1 (MQ=255)
                                                                                                                                               ccggcccggcGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCATCCg  >  3:174227/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTGAGGTTGAAGCGGCCCTGCTCGTCGCGCAGGTGCGCCAGGTCGTAGCCGGTCAGGCAACGGTTGAGCTTGGGGAAGGTGGTTTCAATCAGTTCGGCCTGGGTTTCCTGAATTTCCCGGGCCATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGG‑TTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCATCCG  >  NC_002947/5107345‑5107636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑