Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,171,438 T→A 10.6% M508L (ATG→TTG)  fadD‑I ← long‑chain‑fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,171,4380TA10.6% 124.8 / 6.4 47M508L (ATG→TTG) fadD‑Ilong‑chain‑fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (17/25);  new base A (3/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

GCTCTTCATCACGCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGACCAGGA  >  NC_002947/5171294‑5171584
                                                                                                                                                |                                                                                                                                                  
gCTCTTCATCACGCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGccc                                                                                                                                                >  3:142457/1‑149 (MQ=255)
     tcatcaCGCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCtt                                                                                                                                           >  1:173386/1‑149 (MQ=255)
      catcaCGCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTGACGTTGGCACGCATGTGCTCCATCATCTGCTCCTTGGTCACGGTCATGCCCGGCtt                                                                                                                                           <  6:279088/148‑1 (MQ=255)
            gCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCAc                                                                                                                                     >  1:48820/1‑148 (MQ=255)
               gTTCGCGGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATg                                                                                                                                 <  5:23586/149‑1 (MQ=255)
                  cgcgGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGaa                                                                                                                               <  2:173386/148‑1 (MQ=255)
                   gcggcgCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGa                                                                                                                             <  6:255820/149‑1 (MQ=255)
                         cAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGa                                                                                                                       <  1:321425/149‑1 (MQ=255)
                          aGGCTCTTGCCCACGTGGGTGGTCGGCAGCGAATCGCGGAACTCTATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGAt                                                                                                                      <  2:209825/149‑1 (MQ=255)
                          aGGATGTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGAt                                                                                                                      <  3:50464/149‑1 (MQ=255)
                          aGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGAt                                                                                                                      <  4:44750/149‑1 (MQ=255)
                             aTCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCAc                                                                                                                   >  7:278508/1‑149 (MQ=255)
                                   cacACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGAATGTGCTCCCTCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCAcc                                                                                                             >  6:75348/3‑149 (MQ=255)
                                    ccACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCAccc                                                                                                            >  8:233744/1‑149 (MQ=255)
                                       cGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGACGAAGACCTTGATCACTTCACCCGAc                                                                                                         <  8:272801/149‑1 (MQ=255)
                                            gttgtCGGCAGCTAATCGCGGAACTCGATGTGGGGCGGTACCTTGTAGCCGGTTACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACTATGAAGACCTTGATCACTTCACCCGACTtctc                                                                                                    <  7:315694/146‑1 (MQ=255)
                                               gtCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTc                                                                                                 <  5:196856/149‑1 (MQ=255)
                                                          aTCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGa                                                                                      <  2:48820/149‑1 (MQ=255)
                                                           tCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGAt                                                                                     <  1:269609/149‑1 (MQ=255)
                                                           tCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGAt                                                                                     <  7:233744/149‑1 (MQ=255)
                                                                 aaCTCGATGTCGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCAACTGCTCCTTGGTCACGGTCATGCCAGGCTTGACCACGATGAACACCTTGATAACTTCACCAGACTTCACCTCCGGCACACCGATGGCTgc                                                                               >  5:116343/1‑149 (MQ=255)
                                                                  aCTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTgcg                                                                              >  5:246223/1‑149 (MQ=255)
                                                                                                tAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAac                                                <  8:255681/149‑1 (MQ=255)
                                                                                                      tGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCt                                          >  3:198322/1‑149 (MQ=255)
                                                                                                        gCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTc                                        <  8:278508/149‑1 (MQ=255)
                                                                                                          aCGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCGTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTTCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCa                                      <  1:266637/149‑1 (MQ=255)
                                                                                                                           ccTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACt                                                                          <  8:127991/96‑1 (MQ=255)
                                                                                                                           ccTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACt                                                                          >  7:127991/1‑96 (MQ=255)
                                                                                                                            cTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGt                    >  7:200727/1‑149 (MQ=255)
                                                                                                                                  cTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAg               <  6:246223/148‑1 (MQ=255)
                                                                                                                                  cTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGc              >  7:153191/1‑149 (MQ=255)
                                                                                                                                   ttGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCAGACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGACGCCAACACGTCTTCCTGCTCGTTGGGGTTTACGTTGAAGcc             <  6:116343/149‑1 (MQ=255)
                                                                                                                                   ttGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTACCAGCTCGTTGGCGTAGACGTTGATGcc             >  8:143691/1‑149 (MQ=255)
                                                                                                                                    tGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGccc            >  6:44651/1‑149 (MQ=255)
                                                                                                                                      gTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCTCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCg           <  5:241943/148‑1 (MQ=255)
                                                                                                                                       tcgtggTCAAGCCGGGCATGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGAGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTAGAAGccc            >  7:57743/5‑146 (MQ=255)
                                                                                                                                         aCGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGt                          >  6:12487/1‑130 (MQ=255)
                                                                                                                                         aCGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGt                          <  5:12487/130‑1 (MQ=255)
                                                                                                                                         aCGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGAc       >  6:235543/1‑149 (MQ=255)
                                                                                                                                          tggTCAAGCCGGGCATGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGAcc      >  4:290152/2‑149 (MQ=255)
                                                                                                                                          tggTCAAGCCGGGCATGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGAc       >  4:214985/2‑148 (MQ=255)
                                                                                                                                            gTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGaa                <  5:22156/137‑1 (MQ=255)
                                                                                                                                            gTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGaa                >  6:22156/1‑137 (MQ=255)
                                                                                                                                             tCAAGCCGGGCATGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGACCAgg   <  3:290152/149‑1 (MQ=255)
                                                                                                                                             tCAAGCCGGGCATGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGACCAgg   <  3:214985/149‑1 (MQ=255)
                                                                                                                                               aTGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGACCAGGa  <  8:153191/148‑1 (MQ=255)
                                                                                                                                               aTGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGCCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGACCAGGa  <  5:75348/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCTCTTCATCACGCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGTGGTCGGCAGCGAATCGCGGAACTCGATGTGGCGCGGTACCTTGTAGCCGGTAACGTTGGCACGCATGTGCTCCATCACCTGCTCCTTGGTCACGGTCATGCCCGGCTTGACCACGATGAAGACCTTGATCACTTCACCCGACTTCTCGTCCGGCACACCGATGGCTGCGCACTGCAGCACGCCCGGCAGGGCCGCCAACACGTCTTCCAGCTCGTTGGGGTATACGTTGAAGCCCGAGACCAGGA  >  NC_002947/5171294‑5171584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑