Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,072 A→C 10.6% intergenic (‑130/+24) PP_4560 ← / ← csbD ribonuclease BN/stress response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,0720AC10.6% 171.2 / 12.8 66intergenic (‑130/+24)PP_4560/csbDribonuclease BN/stress response protein
Reads supporting (aligned to +/- strand):  ref base A (39/20);  new base C (4/3);  total (43/23)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

CAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGTT  >  NC_002947/5181925‑5182217
                                                                                                                                                   |                                                                                                                                                 
cAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCgag                                                                                                                                                  <  3:320235/149‑1 (MQ=255)
   gTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaag                                                                                                                                               <  4:4613/149‑1 (MQ=255)
     cGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                                             >  7:315567/1‑149 (MQ=255)
       gggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGCGAAGAGGc                                                                                                                                           <  7:42586/149‑1 (MQ=255)
           aaaTCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCgg                                                                                                                                        >  5:189670/1‑148 (MQ=255)
              tCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCCCGGGTTCACACGAGAAGAGGCCGGCAcc                                                                                                                                    >  3:202261/1‑149 (MQ=255)
               cATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCg                                                                                                                                   >  1:100802/1‑149 (MQ=255)
               cATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCg                                                                                                                                   >  5:44160/1‑149 (MQ=255)
                        tcCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagt                                                                                                                           >  8:19752/1‑148 (MQ=255)
                         ccTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtc                                                                                                                          >  8:65531/1‑148 (MQ=255)
                          cTTTCGCCGCTTGATCGTTACACAGGCAAGTGAAATCGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTGCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtc                                                                                                                          <  5:314441/147‑1 (MQ=255)
                          cTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtcaa                                                                                                                        <  5:285507/149‑1 (MQ=255)
                          cTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtc                                                                                                                          >  6:285507/1‑147 (MQ=255)
                           tttCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtcaag                                                                                                                       >  5:151358/1‑149 (MQ=255)
                           tttCGCCGCTTGATCGTTACACAGGCAAGTCTAAAAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtcaag                                                                                                                       >  5:28689/1‑149 (MQ=255)
                                          gTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCAt                                                                                                        >  3:1570/1‑149 (MQ=255)
                                                 aGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTca                                                                                                  >  1:232583/1‑148 (MQ=255)
                                                  ggCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAc                                                                                                             <  7:104254/136‑1 (MQ=255)
                                                  ggCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAc                                                                                                             >  8:104254/1‑136 (MQ=255)
                                                   gCATGTCTAATTGACCGTGCCCGAGCTGTGCTGGTCCCGGCCGTGGTTGAAGAATTTGCTTACTAATAAGTGCCGGCCTGTTCGCGGGTTCACCCTAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTcac                                                                                                 <  4:202261/147‑1 (MQ=255)
                                                   gCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACAt                                                                                               >  3:222576/1‑149 (MQ=255)
                                                      aGTCTAATATACCGTGCCCGAGCGGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATACGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTc                                                                                            <  6:114572/149‑1 (MQ=255)
                                                       gTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTcc                                                                                           <  8:315567/149‑1 (MQ=255)
                                                         atattAGACCGTCCCCGAGTTGGGCTGCGCCCGGCCTTGGTTGATTAATTTGCTTACTAATAAGTGCCGCCCTGTTCGCGGGTTCACCCTATAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTccc                                                                                          <  6:189670/144‑1 (MQ=255)
                                                             tAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGAc                                                                                     <  2:232583/149‑1 (MQ=255)
                                                                  cGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACctgct                                                                                >  3:174733/1‑149 (MQ=255)
                                                                     gCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTgcg                                                                             >  4:291168/1‑149 (MQ=255)
                                                                     gCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTgcg                                                                             >  5:170581/1‑149 (MQ=255)
                                                                       ccGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGcgcc                                                                           >  7:112723/1‑149 (MQ=255)
                                                                        cGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGcgcct                                                                          <  6:151358/149‑1 (MQ=255)
                                                                               ggctgctCCCGGCCGGGGTTGAAGAATTTGCTTACGAATAAGTCCCGGCGTCTGCGCGGGTTCACCCGAGACGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCttt                                                                   <  4:222576/148‑1 (MQ=255)
                                                                                 ctgctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGa                                                                 <  3:291168/149‑1 (MQ=255)
                                                                                    ccccgggCCGTTGTTTAATATTTTGATTACTAAAAAGTCCCGCCCTCTTCGCGGTTTCACCCGATAATCGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGa                                                                 <  4:14026/141‑1 (MQ=255)
                                                                                     tCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTc                                                             >  5:164901/1‑149 (MQ=255)
                                                                                       ccGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCtt                                                                                        >  4:244303/1‑120 (MQ=255)
                                                                                       ccGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCtt                                                                                        <  3:244303/120‑1 (MQ=255)
                                                                                       ccGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTACt                                                           >  6:219896/1‑149 (MQ=255)
                                                                                                   gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcctt                                               >  3:167943/1‑149 (MQ=255)
                                                                                                   gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcctt                                               >  3:259857/1‑149 (MQ=255)
                                                                                                    aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCTGGTTCAACCGAGAAGAGGCCGGCACAGCCTCAATTCAAGGCTTCTTCACCGCATCATTCACATTTCCCTTGACCTGCTGCGCCTCGACTTTGATCTCCTGCgccttgcct                                                >  7:309195/1‑147 (MQ=255)
                                                                                                     agaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTc                                              <  6:170581/148‑1 (MQ=255)
                                                                                                      cattttGCTTACAAATATGGGCCGGCGTCTGGGGGGTCCCACCCGATAAGCGCCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCgg                                            <  6:28689/146‑1 (MQ=255)
                                                                                                         tttGCTTACTAATAAGGGCGGGCCTCTGCGCGGTTTCACCCGATATGCGCCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGcc                                          <  4:174733/148‑1 (MQ=255)
                                                                                                             cTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAg                                     >  7:227236/1‑149 (MQ=255)
                                                                                                              ttACCAATAAGAGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCAGGCACCGCCTCAAGTCAAGGCATCTTCACCGCATCATTCACATTTCCCTTGACCTGCTGCGGCTCGCCTTTGAGCTCCTGCCCCTAGCCTTCGcccagacgc                                    >  8:141179/1‑143 (MQ=255)
                                                                                                                aCGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtt                                  >  4:165329/1‑149 (MQ=255)
                                                                                                                  gAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCCCCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtttt                                >  3:41887/1‑149 (MQ=255)
                                                                                                                   aaTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTc                               <  6:164901/149‑1 (MQ=255)
                                                                                                                        gTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt                          >  7:110445/1‑149 (MQ=255)
                                                                                                                         tGCCGGCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGAc                                                                                     <  8:276645/89‑1 (MQ=25)
                                                                                                                         tGCCGGCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGAc                                                                                     >  7:276645/1‑89 (MQ=25)
                                                                                                                         tGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTc                         >  3:245441/1‑149 (MQ=255)
                                                                                                                           ccGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCgg                       >  7:45678/1‑149 (MQ=255)
                                                                                                                             ggCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTc                     >  4:3363/1‑149 (MQ=255)
                                                                                                                             ggCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt                      >  4:80001/1‑148 (MQ=255)
                                                                                                                               ccTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                        >  6:333581/1‑144 (MQ=255)
                                                                                                                               ccTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                        <  5:333581/144‑1 (MQ=255)
                                                                                                                               ccTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGGCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCAc                   >  7:73716/1‑149 (MQ=255)
                                                                                                                                   ttCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGACTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtag               >  7:287583/1‑147 (MQ=255)
                                                                                                                                   ttCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                        <  6:144255/140‑1 (MQ=255)
                                                                                                                                   ttCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                        >  5:144255/1‑140 (MQ=255)
                                                                                                                                     cgcgGGTTCACCCTATAATAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGc              <  8:45678/148‑1 (MQ=255)
                                                                                                                                              aCCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTg    >  5:44000/1‑149 (MQ=255)
                                                                                                                                                ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGGCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGtt  >  5:292456/1‑149 (MQ=255)
                                                                                                                                                ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt                <  4:100510/135‑1 (MQ=255)
                                                                                                                                                ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt                >  3:100510/1‑135 (MQ=255)
                                                                                                                                                ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGtt  >  6:168157/1‑149 (MQ=255)
                                                                                                                                                ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGtt  >  4:157861/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGTT  >  NC_002947/5181925‑5182217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑