Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,854 G→T 7.6% G115V (GGC→GTC)  PP_4562 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,8540GT 7.6% 206.4 / 6.6 66G115V (GGC→GTC) PP_4562hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/32);  new base T (3/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

GAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAAC  >  NC_002947/5182706‑5182993
                                                                                                                                                    |                                                                                                                                           
gAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTgg                                                                                                                                             >  8:72992/1‑149 (MQ=255)
  gCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGc                                                                                                                                            >  4:186356/1‑148 (MQ=255)
   cTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGa                                                                                                                                          <  6:28448/149‑1 (MQ=255)
   cTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGa                                                                                                                                          <  3:186356/149‑1 (MQ=255)
     tgggcaCCTTCAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGag                                                                                                                                         >  4:104828/4‑148 (MQ=255)
     gcagcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGag                                                                                                                                         >  2:178330/1‑148 (MQ=255)
      cagcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCTCGCCACCCGGACGGGTGTCgcgc                                                                                                                                        <  4:310197/148‑1 (MQ=255)
      cagcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGagca                                                                                                                                       <  5:248077/149‑1 (MQ=255)
      cagcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGag                                                                                                                                         <  4:9902/147‑1 (MQ=255)
         caCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCt                                                                                                                                    >  2:183689/1‑149 (MQ=255)
          aCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTg                                                                                                                                   <  7:72992/149‑1 (MQ=255)
            cTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTg                                                                                                                                   >  6:295737/1‑147 (MQ=255)
            cTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTg                                                                                                                                   <  5:295737/147‑1 (MQ=255)
             tGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCCCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGCCCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGAc                                                                                                                                >  5:28352/1‑149 (MQ=255)
                 ggTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTcc                                                                                                                            <  3:104828/149‑1 (MQ=255)
                         ccaATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTc                                                                                                                    <  1:178330/149‑1 (MQ=255)
                           aaTTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCg                                                                                                                   >  5:253027/1‑148 (MQ=255)
                            aTTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCTCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTa                                                                                                                 <  4:59455/149‑1 (MQ=255)
                             tttCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTa                                                                                                                 >  3:90634/1‑148 (MQ=255)
                              ttCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTa                                                                                                                 >  3:201663/1‑147 (MQ=255)
                                 cTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGc                                                                                                            <  1:183689/149‑1 (MQ=255)
                                  tCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCg                                                                                                           >  3:212161/1‑149 (MQ=255)
                                      ggCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAg                                                                                                       >  7:223725/1‑149 (MQ=255)
                                         tCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGcc                                                                                                    >  6:218683/1‑149 (MQ=255)
                                          cACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTCCAGGCGTAAGGCCg                                                                                                   >  8:250634/1‑149 (MQ=255)
                                           aCCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGcc                                                                                                    <  8:223725/147‑1 (MQ=255)
                                            ccTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGt                                                                                                  >  3:112968/1‑148 (MQ=255)
                                                gAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAg                                                                                               <  4:201663/147‑1 (MQ=255)
                                                 agaaaaTGGAAGTGCGCAAGCCGGGCAAGCGCCGCTCCACCTTCAGCCTGGACCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACAACCGTCCGGGTGGCTAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGc                                                                                             <  6:92698/145‑1 (MQ=255)
                                                 aGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGc                                                                                             <  4:212161/148‑1 (MQ=255)
                                                   cAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTTCGCGACACCCGTCCGGGTGGCGAGCAGCTGGCCATCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCtt                                                                                           >  3:274791/1‑148 (MQ=255)
                                                     aaTGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTtct                                                                                        <  5:174794/149‑1 (MQ=255)
                                                       tGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTtctc                                                                                       >  7:226908/1‑148 (MQ=255)
                                                       tGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTtctc                                                                                       >  7:43970/1‑148 (MQ=255)
                                                         gAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGa                                                                                     <  6:19420/148‑1 (MQ=255)
                                                         gAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGAt                                                                                    <  4:4893/149‑1 (MQ=255)
                                                          aaGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATg                                                                                   >  8:38766/1‑149 (MQ=255)
                                                                gcAAGCCTGGCAAGCGCCGCTCAACAATCAGCCTGGCCCTAAGCGCCGAACGGGACTCGCTGCTGCGAGACACCCGTCCAGCTGCCGAGCACCTGGACTTCCTCAACATCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATCCAATc                                                                              >  7:120812/1‑148 (MQ=255)
                                                                 cAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGt                                                                            >  2:87548/1‑149 (MQ=255)
                                                                     ccTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATg                                                                        >  6:180817/1‑149 (MQ=255)
                                                                              cgccgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGAt                                                               <  1:87548/149‑1 (MQ=255)
                                                                               gccgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTTGATGCGATCGTCATGGATGACGAt                                                               >  5:338787/1‑148 (MQ=255)
                                                                               gccgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGAt                                                               >  8:181502/1‑148 (MQ=255)
                                                                                ccgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCa                                                             <  8:43970/149‑1 (MQ=255)
                                                                                   cTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAAc                                                                                                                       >  5:310394/1‑88 (MQ=255)
                                                                                   cTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAAc                                                                                                                       <  6:310394/88‑1 (MQ=255)
                                                                                       accaTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTg                                                      <  5:218683/149‑1 (MQ=255)
                                                                                             aGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGaa                                                <  7:38766/149‑1 (MQ=255)
                                                                                                cTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGaacaa                                             <  6:253027/149‑1 (MQ=255)
                                                                                                   gCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAAccc                                          <  7:195734/149‑1 (MQ=255)
                                                                                                   gCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAAcc                                           <  7:120309/148‑1 (MQ=255)
                                                                                                       tCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATc                                                                              <  8:249081/109‑1 (MQ=255)
                                                                                                       tCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATc                                                                              >  7:249081/1‑109 (MQ=255)
                                                                                                          gcgcCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATg                                   <  4:112968/149‑1 (MQ=255)
                                                                                                               gACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGGTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGTCctt                              >  5:199413/1‑149 (MQ=255)
                                                                                                                  cGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCcttctt                           >  1:47435/1‑149 (MQ=255)
                                                                                                                  cGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCcttctt                           <  2:47435/149‑1 (MQ=255)
                                                                                                                          gctgctGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGtt                     <  8:226908/147‑1 (MQ=255)
                                                                                                                          gctgctGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGc                   >  1:159901/1‑149 (MQ=255)
                                                                                                                          gctgctGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGc                   <  4:274791/149‑1 (MQ=255)
                                                                                                                              ctgcTCGCCACCCGGACGGGTGTCGCGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCtt               >  3:2099/1‑149 (MQ=255)
                                                                                                                               tgcTCGCCACCCGGACGGGTGTCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTg              >  3:266363/1‑149 (MQ=255)
                                                                                                                                gctcgccacCCGGACGGGTGTCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGc                                        <  5:141710/116‑1 (MQ=255)
                                                                                                                                gctcgccacCCGGACGGGTGTCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGc                                        >  6:141710/7‑122 (MQ=255)
                                                                                                                                    gACACCCTTCCGGGTTGCGAGCAGCTGGACTTCCGCAACTTCGTAGAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGAc         <  6:199413/149‑1 (MQ=255)
                                                                                                                                    gACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGAc         >  4:206632/1‑149 (MQ=255)
                                                                                                                                           gTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAAc  <  2:159901/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
GAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAAC  >  NC_002947/5182706‑5182993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: