Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	342,201	G→A	6.1%	V240I (GTA→ATA)	gabP‑I →	gamma‑aminobutyrate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	342,201	0	G	A	6.1%	193.2 / 2.0	66	V240I (GTA→ATA)	gabP‑I	gamma‑aminobutyrate permease
Reads supporting (aligned to +/- strand):  ref base G (33/28);  new base A (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

AGGGCTTCATGCCCAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGCTGATCGTCGA  >  NC_002947/342058‑342349                                                                                                                                                |                                                                                                                                                      aGGGCTTCATGCCCAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTaa                                                                                                                                                    <  5:170626/147‑1 (MQ=255) aGGGCTTCATGCCCAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGACGAAGGACCCGGGCAAGCAGATCCGCCGGGCCACCAACTCGGTaa                                                                                                                                                    >  6:170626/1‑147 (MQ=255)  gggCTTCATGCCCAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACACGGGCAAGCAGATCAGACGTGCAACCAACTCGGTAATCt                                                                                                                                                 >  2:321361/1‑149 (MQ=255)     cTTCATGCCCAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGc                                                                                                                                              <  3:92017/149‑1 (MQ=255)     attCATGCCCAACTGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCTTGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGc                                                                                                                                              <  1:321361/148‑1 (MQ=255)        cATGCCCAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTa                                                                                                                                           >  4:230330/1‑149 (MQ=255)             ccAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGc                                                                                                                                      >  3:162132/1‑149 (MQ=255)              cAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGcc                                                                                                                                     >  4:177191/1‑149 (MQ=255)                aCGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCt                                                                                                                                        >  4:130525/1‑144 (MQ=255)                aCGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCt                                                                                                                                        <  3:130525/144‑1 (MQ=255)                   gCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGATAATCTGGCGTATCTGCCTGTTc                                                                                                                                >  7:314185/1‑149 (MQ=255)                     cTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTa                                                                                                                              >  5:255073/1‑149 (MQ=255)                       gggCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCt                                                                                                                                    >  8:96362/1‑141 (MQ=255)                       gggCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCt                                                                                                                                    <  7:96362/141‑1 (MQ=255)                       gggCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTAcc                                                                                                                            >  6:119243/1‑149 (MQ=255)                          cgcgGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTgg                                                                                                                         >  4:166211/1‑149 (MQ=255)                               tGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATc                                                                                                                     >  4:263782/1‑148 (MQ=255)                                   ggCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAAATCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCt                                                                                                                >  4:201001/1‑149 (MQ=255)                                    gCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTgg                                                                                                                         >  4:81849/1‑139 (MQ=255)                                    gCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTgg                                                                                                                         <  3:81849/139‑1 (MQ=255)                                        ccATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGATAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTg                                                                                                           <  8:314185/149‑1 (MQ=255)                                          aTGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTg                                                                                                                          <  2:221389/132‑1 (MQ=255)                                          aTGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTg                                                                                                                          >  1:221389/1‑132 (MQ=255)                                                  caccaTGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGATAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGccc                                                                                                >  8:286294/1‑149 (MQ=255)                                                    ccaTGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGCTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTTGCACTg                                                                                              >  8:166701/1‑149 (MQ=255)                                                           ctcGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCg                                                                                        <  6:255073/148‑1 (MQ=255)                                                           ctcGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGt                                                                                       >  8:99869/1‑149 (MQ=255)                                                                      gTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCg                                                                            >  7:18728/1‑149 (MQ=255)                                                                       tACCCAAATCGTCCCCCTCGCCGCTGCCTAGTCGAAGGACCCTGGCAAGCAGAGCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCgg                                                                           <  8:281113/149‑1 (MQ=255)                                                                            aaaTCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTTGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTg                                                                      <  4:162132/149‑1 (MQ=255)                                                                             aaTCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTCTGTGGCCCTGGTGCCGTGGAACGACCCGGCCCTg                                                                      <  1:40496/149‑1 (MQ=255)                                                                              aTCGTCCCCATCGCCGCTGCCGAGAGGAAGGACCGGTGCTAGCAGATCACCCGTGCCACCAACTAGGTAATCTGGCGTAGCTGCCTGTTCTACCTGGTCTCGTTCTTGCTGGTC‑GTGGCCGTGGTGGCGTGGAACGACCCGGCCCTGGc                                                                    <  3:74883/149‑1 (MQ=255)                                                                              aTCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGc                                                                    <  3:166211/149‑1 (MQ=255)                                                                                 gTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGcc                                                                   >  1:318468/1‑147 (MQ=255)                                                                                  tCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGcc                                                                   >  3:158799/1‑146 (MQ=255)                                                                                    aCCATCGCCGCTGCCGAGTCTAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGATAATCTGGCGGATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGAc                                                              <  7:286294/149‑1 (MQ=255)                                                                                     ccATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACg                                                             >  6:320825/1‑149 (MQ=255)                                                                                     ccATCGCCGCTGCCGAGTCGAAGGACACGGGCAAGCAGATAAGCCCTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCATCCTGGTC‑GTGGCCCTCCTGCCCTGGAACGACCCGGCCCTGGCCGAGACg                                                             >  8:13156/1‑149 (MQ=255)                                                                                      cATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACgg                                                            <  7:99869/149‑1 (MQ=255)                                                                                       aTCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACggg                                                           <  4:288739/149‑1 (MQ=255)                                                                                          gccgcTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTc                                                        <  8:18728/149‑1 (MQ=255)                                                                                             gcTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTa                                                     <  5:119243/149‑1 (MQ=255)                                                                                                 ccGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGcc                                                                                         <  4:35416/109‑1 (MQ=255)                                                                                                 ccGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGcc                                                                                         >  3:35416/1‑109 (MQ=255)                                                                                                    aGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGAcc                                              >  8:70264/1‑149 (MQ=255)                                                                                                      tCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGATCCTACCAGACCGt                                            >  7:128888/1‑149 (MQ=255)                                                                                                        gAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGc                                          >  4:322022/1‑149 (MQ=255)                                                                                                         aaGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTAGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGc                                          <  2:260638/148‑1 (MQ=255)                                                                                                         aaGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTAGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGc                                          >  1:260638/1‑148 (MQ=255)                                                                                                           ggACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGa                                       <  7:13156/149‑1 (MQ=255)                                                                                                             aCCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGc                                     <  4:158799/149‑1 (MQ=255)                                                                                                             aCCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGc                                     <  3:201001/149‑1 (MQ=255)                                                                                                             aCCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGc                                     <  3:230330/149‑1 (MQ=255)                                                                                                               ccGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCg                                   >  3:3940/1‑149 (MQ=255)                                                                                                                     aaGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCgg                             <  8:128888/149‑1 (MQ=255)                                                                                                                     aaGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCgg                             >  2:98073/1‑149 (MQ=255)                                                                                                                         aGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGtgtg                         >  4:101107/1‑149 (MQ=255)                                                                                                                             cAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGCTGTGCCg                      >  7:141069/1‑148 (MQ=255)                                                                                                                                ccGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGcc                                    <  6:215294/131‑1 (MQ=255)                                                                                                                                ccGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGcc                                    >  5:215294/1‑131 (MQ=255)                                                                                                                                  gTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGgtgt                          >  3:164914/1‑139 (MQ=255)                                                                                                                                  gTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGgtgt                          <  4:164914/139‑1 (MQ=255)                                                                                                                                     ccaccaACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAg             <  3:101107/149‑1 (MQ=255)                                                                                                                                      caccaACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGc            <  4:164862/149‑1 (MQ=255)                                                                                                                                          aaCTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGCTGAt        >  5:254464/1‑149 (MQ=255)                                                                                                                                            cTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGCTGAtcg      >  7:76196/1‑149 (MQ=255)                                                                                                                                               ggTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGCTGAtcgtc    <  2:175191/148‑1 (MQ=255)                                                                                                                                               ggTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGCTGAtcgt     <  3:237613/147‑1 (MQ=255)                                                                                                                                                gTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGCTGATCGTCGa  >  8:126719/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                      AGGGCTTCATGCCCAACGGCCTGGGCGCGGTGCTGGCAGCCATGCTGACCACCATGTTCTCGTTCATGGGTACCGAAATCGTCACCATCGCCGCTGCCGAGTCGAAGGACCCGGGCAAGCAGATCAGCCGTGCCACCAACTCGGTAATCTGGCGTATCTGCCTGTTCTACCTGGTATCGATCTTCCTGGTC‑GTGGCCCTGGTGCCGTGGAACGACCCGGCCCTGGCCGAGACGGGCTCCTACCAGACCGTGCTGAGCCGTATCGGTGTGCCGAATGCCAAGCTGATCGTCGA  >  NC_002947/342058‑342349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑