Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,302,665 T→C 7.9% S370G (AGC→GGC)  recB ← Chi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,302,6650TC 7.9% 181.2 / 5.4 63S370G (AGC→GGC) recBChi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease
Reads supporting (aligned to +/- strand):  ref base T (32/26);  new base C (2/3);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.57e-01

AACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTTGCTG  >  NC_002947/5302517‑5302812
                                                                                                                                                    |                                                                                                                                                   
aaCAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCt                                                                                                                                                     >  8:269225/1‑149 (MQ=255)
      ccGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCTCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCAGCGAGGCCGGCGAg                                                                                                                                               <  6:199396/149‑1 (MQ=255)
      ccGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCAGCGAGGCCGGCGAg                                                                                                                                               <  6:147825/149‑1 (MQ=255)
       cGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGTCGGCCTCGCTGGCGAgc                                                                                                                                              >  4:18776/1‑149 (MQ=255)
       cGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAgc                                                                                                                                              <  7:2821/149‑1 (MQ=255)
       cGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGACCAGGCGCTCGCCAGCGAGGCCGGCGAgc                                                                                                                                              <  3:278885/149‑1 (MQ=255)
          tttCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGcgcg                                                                                                                                           >  5:204523/1‑149 (MQ=255)
              tGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAgcgc                                                                                                                                            <  4:120011/144‑1 (MQ=255)
              tGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAgcgc                                                                                                                                            >  3:120011/1‑144 (MQ=255)
              tGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtgct                                                                                                                                       >  7:265366/1‑149 (MQ=255)
                cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtgctg                                                                                                                                      >  6:326101/1‑148 (MQ=255)
                cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtgct                                                                                                                                       <  8:123834/147‑1 (MQ=255)
                cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtgc                                                                                                                                        <  8:206085/146‑1 (MQ=255)
                           cTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAg                                                                                                                          >  8:158013/1‑149 (MQ=255)
                           cTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAg                                                                                                                          >  8:261728/1‑149 (MQ=255)
                                  ggTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtcg                                                                                                                   >  7:95244/1‑149 (MQ=255)
                                          cgcTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGc                                                                                                            <  5:182256/148‑1 (MQ=255)
                                          cgcTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGc                                                                                                            <  8:95244/148‑1 (MQ=255)
                                            cTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCa                                                                                                         >  8:142841/1‑149 (MQ=255)
                                             tCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAt                                                                                                        >  7:27544/1‑149 (MQ=255)
                                               gAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAttt                                                                                                      >  2:198678/1‑149 (MQ=255)
                                                   aTGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGc                                                                                                  <  7:142841/149‑1 (MQ=255)
                                                     gCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCAc                                                                                                >  6:163806/1‑149 (MQ=255)
                                                      ccGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGa                                                                                                                                                >  3:285478/1‑100 (MQ=255)
                                                      ccGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGa                                                                                                                                                <  4:285478/100‑1 (MQ=255)
                                                         tACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCg                                                                                            >  5:63724/1‑149 (MQ=255)
                                                           cTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGgc                                                                                          >  7:114741/1‑149 (MQ=255)
                                                            tGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGgcg                                                                                         >  3:185122/1‑149 (MQ=255)
                                                                    gTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCa                                                                                 >  5:340922/1‑149 (MQ=255)
                                                                       ggTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACtt                                                                              <  6:204523/149‑1 (MQ=255)
                                                                            ccTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGaaa                                                                         <  7:269225/149‑1 (MQ=255)
                                                                             cTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAAc                                                                        >  7:157355/1‑149 (MQ=255)
                                                                                  aTTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGcc                                                                   <  7:261728/149‑1 (MQ=255)
                                                                                  aTTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGcc                                                                   <  7:158013/149‑1 (MQ=255)
                                                                                           tCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGTCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCaa                                                          <  3:18776/149‑1 (MQ=255)
                                                                                              gcgcTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTg                                                       >  4:261889/1‑149 (MQ=255)
                                                                                                  tACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcgg                                                   >  4:251438/1‑149 (MQ=255)
                                                                                                    ccGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcggc                                                  <  8:35855/148‑1 (MQ=255)
                                                                                                    ccGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcggc                                                  <  5:326101/148‑1 (MQ=255)
                                                                                                        gAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCt                                             <  7:43254/149‑1 (MQ=255)
                                                                                                         aaCTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTc                                            >  1:134561/1‑149 (MQ=255)
                                                                                                         aaCTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTc                                            <  4:70791/149‑1 (MQ=255)
                                                                                                           cTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGa                                          <  4:185122/149‑1 (MQ=255)
                                                                                                            tGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGag                                         >  3:273241/1‑149 (MQ=255)
                                                                                                              cTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGag                                         <  1:300577/147‑1 (MQ=255)
                                                                                                               tCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGagcag                                      >  8:289033/1‑149 (MQ=255)
                                                                                                                cACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGagcagc                                     >  1:108270/1‑149 (MQ=255)
                                                                                                                 aCGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCg                                    >  7:117860/1‑149 (MQ=255)
                                                                                                                  cGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCgg                                   <  4:273241/149‑1 (MQ=255)
                                                                                                                   ggATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCggg                                  <  3:261889/149‑1 (MQ=255)
                                                                                                                          gCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCg                           >  5:184972/1‑149 (MQ=255)
                                                                                                                           cTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCgg                          <  6:184972/149‑1 (MQ=255)
                                                                                                                            tGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCggg                         >  4:281413/1‑149 (MQ=255)
                                                                                                                              gCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCggg                         >  8:304653/1‑147 (MQ=255)
                                                                                                                               ccAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGTACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATc                             >  4:112473/1‑142 (MQ=255)
                                                                                                                               ccAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGTACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATc                             <  3:112473/142‑1 (MQ=255)
                                                                                                                               ccAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCggg                         >  3:219205/1‑146 (MQ=255)
                                                                                                                               ccAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCggg                         <  4:219205/146‑1 (MQ=255)
                                                                                                                                 acgcgcTCGCCAGCGAGGCCGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTc                    >  1:265654/3‑149 (MQ=255)
                                                                                                                                 aGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTc                    <  6:340922/149‑1 (MQ=255)
                                                                                                                                     gcTCGCCAGCGAGGCCGGCGAGCGCCTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAgg                >  5:150272/1‑149 (MQ=255)
                                                                                                                                        cGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGcc             >  2:44130/1‑149 (MQ=255)
                                                                                                                                                  gCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTtgct   <  8:157355/149‑1 (MQ=255)
                                                                                                                                                   cTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCGGTtgctg  >  5:120496/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTTGCTG  >  NC_002947/5302517‑5302812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑