| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 343,607 | T→A | 6.6% | intergenic (‑163/‑77) | PP_5439 ← / → PP_5440 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 343,607 | 0 | T | A | 6.6% | 180.0 / 3.0 | 61 | intergenic (‑163/‑77) | PP_5439/PP_5440 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (29/28); new base A (2/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01 | |||||||||||
TGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGTTTC > NC_002947/343460‑343755 | tGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATg > 4:14739/1‑149 (MQ=255) tGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTtggct > 6:61686/1‑149 (MQ=255) tGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGc < 8:86577/149‑1 (MQ=255) tGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGc < 3:14739/149‑1 (MQ=255) cgcgTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCt < 6:151139/149‑1 (MQ=255) cgcgTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCAGca < 2:101923/148‑2 (MQ=255) cgTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCAGcagga < 5:245053/149‑5 (MQ=255) aTTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGAACGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCAc > 1:22149/1‑149 (MQ=255) gAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTtggc > 4:257179/1‑132 (MQ=255) gAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTtggc < 3:257179/132‑1 (MQ=255) cccGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGc < 5:61686/148‑1 (MQ=255) cGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTc > 1:254995/1‑149 (MQ=255) gATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTcc > 1:303369/1‑149 (MQ=255) gATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTcc > 6:247124/1‑149 (MQ=255) gATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTcc > 1:201171/1‑149 (MQ=255) aaGCGGCGCGGCTGGGCGCTGCAGGTTGAGATGTCTGGGGGATTCATATCCAGATTATTCGGACCGTGGGCCCTGCGTTGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGAt < 2:334560/149‑1 (MQ=255) gcggcgCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATgc < 2:254995/149‑1 (MQ=255) ggcgCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATgcg > 4:56439/1‑148 (MQ=255) gcgcgcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCg < 8:17269/129‑1 (MQ=255) gcgcgcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCg > 7:17269/1‑129 (MQ=255) cgcgcCTGGGCGCGGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCAc < 6:60099/149‑1 (MQ=255) cgcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGt < 4:157591/149‑1 (MQ=255) gggCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtga < 1:313959/131‑1 (MQ=255) gggCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtga > 2:313959/1‑131 (MQ=255) tGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTgcgc < 6:20920/108‑1 (MQ=255) tGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTgcgc > 5:20920/1‑108 (MQ=255) gaTGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGc > 8:290694/1‑86 (MQ=255) gaTGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGc < 7:290694/86‑1 (MQ=255) tACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACtttt < 1:282455/149‑1 (MQ=255) tACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACtt < 6:144842/147‑1 (MQ=255) tACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACtt > 5:144842/1‑147 (MQ=255) atatCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATc > 8:137936/1‑149 (MQ=255) cAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGttt > 1:15069/1‑109 (MQ=255) cAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGttt < 2:15069/109‑1 (MQ=255) attattCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCAcc > 8:187307/1‑149 (MQ=255) gACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtga < 1:171579/83‑1 (MQ=255) gACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtga > 2:171579/1‑83 (MQ=255) tGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGTAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTaca < 7:76224/149‑1 (MQ=255) tGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGTAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTaca > 8:76224/1‑149 (MQ=255) cccTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGa > 4:22666/1‑149 (MQ=255) cTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAgg < 3:94894/149‑1 (MQ=255) cTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAgg > 6:33442/1‑149 (MQ=255) ttCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAggg > 3:61072/1‑149 (MQ=255) tctctcCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGt > 5:336679/1‑149 (MQ=255) ctgctgCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGa < 8:187897/131‑1 (MQ=255) ctgctgCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGa > 7:187897/1‑131 (MQ=255) ctgctgCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTa < 5:247124/146‑1 (MQ=255) gctgcCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTAggg > 2:134103/1‑120 (MQ=255) gctgcCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACgg < 1:134103/120‑1 (MQ=255) gctgcCAGCCAACATGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATc > 2:180370/1‑149 (MQ=255) gctgcCACCCAACATGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTCGTCACCCTGAACGGCTACACGGACGATGGCAGGGTTTACTATc > 4:311217/1‑149 (MQ=255) tgcCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTAt < 7:61038/146‑1 (MQ=255) tgcCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTAt > 8:61038/1‑146 (MQ=255) ccagccaTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTa > 5:320036/1‑119 (MQ=255) ccagccaTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTa < 6:320036/119‑1 (MQ=255) ccagccaTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAggg > 7:7600/1‑136 (MQ=255) ccagccaTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAggg < 8:7600/136‑1 (MQ=255) cagccaTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAAcc < 7:137936/149‑1 (MQ=255) caTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGtt < 4:61072/149‑1 (MQ=255) caTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGtt > 8:92963/1‑149 (MQ=255) aTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCTCGTTTGCGCTACAAAGGAGGTGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGttt < 2:22149/149‑1 (MQ=255) tGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGGCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGTTTc < 2:201171/149‑1 (MQ=255) | TGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGTTTC > NC_002947/343460‑343755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |