Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,342,614 T→C 12.4% intergenic (+60/‑147) panC → / → pgi‑II pantothenate synthetase/glucose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,342,6140TC12.4% 120.5 / 7.1 48intergenic (+60/‑147)panC/pgi‑IIpantothenate synthetase/glucose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (16/26);  new base C (2/4);  total (18/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGGGAACCCC  >  NC_002947/5342466‑5342755
                                                                                                                                                    |                                                                                                                                             
gagaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGt                                                                                                                                               <  8:19942/149‑1 (MQ=255)
 agaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTa                                                                                                                                              <  6:313846/149‑1 (MQ=255)
 agaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTa                                                                                                                                              >  2:257250/1‑149 (MQ=255)
     cTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAgggg                                                                                                                                          >  7:84638/1‑149 (MQ=255)
        gtggtgATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCAAGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcgg                                                                                                                                       <  6:248063/149‑1 (MQ=37)
              aTTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGgcttgc                                                                                                                                 <  6:40790/149‑1 (MQ=255)
              aTTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGGAGCGGgcttgc                                                                                                                                 <  8:43176/149‑1 (MQ=38)
                  cggcggCTTACCTGGGAAACACGAGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCttgcttgc                                                                                                                             <  7:273316/149‑1 (MQ=255)
                   ggcggcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCAGCCCGGTTACCTGTAGGGGCGGGCttgcttgc                                                                                                                             >  6:256825/1‑148 (MQ=255)
                    gcggcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCaa                                                                                                                           <  8:84638/149‑1 (MQ=255)
                       gcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGc                                                                                                                        <  3:179487/149‑1 (MQ=255)
                             cTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcgg                                                                                                                                       >  8:8664/1‑128 (MQ=38)
                             cTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcgg                                                                                                                                       <  7:8664/128‑1 (MQ=38)
                               gggTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCaaa                                                                                                                 >  4:238987/1‑148 (MQ=255)
                                            ttGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATa                                                                                                   <  7:185515/149‑1 (MQ=255)
                                              gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATAc                                                                                                  <  3:99585/148‑1 (MQ=255)
                                                tCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTTCAGCCCATTCGCGGGCAAACCCCCTCCTGGACGTTTAACTGTAGGGGCGGGCTTGCTTGCAATTCCCCCAAAATCCCCCAGGCAGAccc                                                                                                >  1:137851/1‑148 (MQ=255)
                                                 ctaCAACCTCTACCTGCATTTGGAAGAGAATACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCTGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCAt                                                                                              <  6:279208/147‑1 (MQ=255)
                                                    cAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTcc                                                                                           <  1:312804/149‑1 (MQ=255)
                                                           tACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCAc                                                                                     <  3:268775/148‑1 (MQ=255)
                                                             ccTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGt                                                                                   <  6:118989/148‑1 (MQ=255)
                                                                            gaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCTCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAAt                                                                   >  7:220139/1‑149 (MQ=255)
                                                                            gaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATaa                                                                    <  1:257250/148‑1 (MQ=255)
                                                                               gaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGt                                                                <  1:307434/149‑1 (MQ=255)
                                                                                  cGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCCAAATCCCCCTGCCCTACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGcc                                                             >  7:71379/1‑149 (MQ=255)
                                                                                     aTAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGc                                                          <  5:256825/149‑1 (MQ=255)
                                                                                        aCGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCt                                                        <  1:11947/148‑1 (MQ=255)
                                                                                        aCGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCt                                                        <  1:77603/148‑1 (MQ=255)
                                                                                           gTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCAc                                                                                     <  5:229510/116‑1 (MQ=255)
                                                                                                ggtcTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGc                                                          <  3:275374/135‑1 (MQ=255)
                                                                                                gggCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTGGCAATGCCCCCAAACTCCCCCTGCCATCCCCATTCCCCGCACGTGGCCTTTGCCTATAATGGTGCCAGc                                                          <  3:279931/138‑1 (MQ=255)
                                                                                                  gCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTCGCCTATAATGGTGCCAGCCTGAACCCGGCaa                                             >  3:203498/1‑149 (MQ=255)
                                                                                                   cTGCCTTGCATCCCATTCGCGGGCAAGCCCGCTCCTGCCCGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGTCTATAATTGTGCCAGCCTGAAACCGGCAAc                                            <  2:249899/149‑1 (MQ=255)
                                                                                                           cAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGGACCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGGCTATAAAGGTGCCGGCCTGAACCCCGCACCGACTGCCg                                    >  4:105554/1‑149 (MQ=255)
                                                                                                               ccATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCCCGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGGGTc                                >  5:237540/1‑149 (MQ=255)
                                                                                                               ccATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTc                                <  3:238987/149‑1 (MQ=255)
                                                                                                                  ttCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGc                                                                                                       >  4:133224/1‑75 (MQ=255)
                                                                                                                  ttCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGc                                                                                                       <  3:133224/75‑1 (MQ=255)
                                                                                                                               ccGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTGCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACg                 >  7:62308/1‑148 (MQ=255)
                                                                                                                               ccGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGcc                                                         <  4:59579/108‑1 (MQ=255)
                                                                                                                               ccGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGcc                                                         >  3:59579/1‑108 (MQ=255)
                                                                                                                                     cTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCaa           <  8:220139/148‑1 (MQ=255)
                                                                                                                                      tGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATGCCCCTGCCAGACCCATTACCAGCACGTGGGCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCCCCCCCCACCAAgg         >  6:107407/1‑149 (MQ=255)
                                                                                                                                      tGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAgg         >  1:306080/1‑149 (MQ=255)
                                                                                                                                        cACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTCGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAggg        <  4:203498/148‑1 (MQ=255)
                                                                                                                                          cGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGGGAAc     <  2:137851/149‑1 (MQ=255)
                                                                                                                                           gTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTACCAGCACGTGGCCGTTGCCTATAATTGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCCCCCCGCACCAAGGGAAcc    >  1:173462/1‑149 (MQ=255)
                                                                                                                                                ccTGCAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGGGAAcccc  <  2:265240/146‑1 (MQ=255)
                                                                                                                                                ccTGCAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGGGAAcccc  >  1:265240/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGGGAACCCC  >  NC_002947/5342466‑5342755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑