Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,346,102 C→G 7.0% R500R (CGC→CGG)  acsA‑II → acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,346,1020CG 7.0% 182.0 / 3.9 57R500R (CGC→CGG) acsA‑IIacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base G (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

AAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCC  >  NC_002947/5345960‑5346243
                                                                                                                                              |                                                                                                                                             
aaGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGcc                                                                                                                                         >  3:250200/1‑149 (MQ=255)
             cggcgTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGGCATCGCGACgggc                                                                                                                                  <  8:62715/143‑3 (MQ=255)
             cggcgTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGGCATCGCGACgggc                                                                                                                                  >  7:62715/1‑141 (MQ=255)
                    cTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCAc                                                                                                                     >  1:311031/1‑149 (MQ=255)
                    cTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCAc                                                                                                                     >  6:88722/1‑149 (MQ=255)
                      ggCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCg                                                                                                                   <  5:88722/149‑1 (MQ=255)
                           tCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGGCATCGCGACgggca                                                                                                                                 >  5:45352/1‑127 (MQ=39)
                           tCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGGCATCGCGACgggca                                                                                                                                 <  6:45352/130‑4 (MQ=39)
                             aaaGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCTGACGGCGACTACTGGATCACCGGGCGCAt                                                                                                            <  1:25582/149‑1 (MQ=255)
                                gCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATcga                                                                                                         >  6:31238/1‑149 (MQ=255)
                                 ccAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATcgac                                                                                                        >  3:291976/1‑149 (MQ=255)
                                  cAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAG‑CCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATcgacga                                                                                                      <  8:223617/149‑1 (MQ=255)
                                         cccGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGAt                                                                                                <  4:134625/149‑1 (MQ=255)
                                         cccGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGAt                                                                                                >  2:225969/1‑149 (MQ=255)
                                            gggCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCaa                                                                                             <  5:31238/149‑1 (MQ=255)
                                                  aTCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTg                                                                                                                           >  6:54391/1‑113 (MQ=255)
                                                  aTCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTg                                                                                                                           <  5:54391/113‑1 (MQ=255)
                                                   tCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTcc                                                                                      >  1:321380/1‑149 (MQ=255)
                                                       cAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGc                                                                                  >  4:189803/1‑149 (MQ=255)
                                                             aggtgtcGACCATCCGCTGGTGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCa                                                                            >  6:192521/7‑149 (MQ=37)
                                                                      ccaccaGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTcc                                                                                      <  7:149757/130‑1 (MQ=255)
                                                                      ccaccaGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTcc                                                                                      >  8:149757/1‑130 (MQ=255)
                                                                       caccaGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGc                                                                  >  3:147851/1‑149 (MQ=255)
                                                                          caGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTg                                                                >  2:173296/1‑148 (MQ=255)
                                                                           aGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGaa                                                              <  4:250200/149‑1 (MQ=255)
                                                                                  ggTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCa                                                                                              <  2:237917/110‑1 (MQ=255)
                                                                                  ggTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCa                                                                                              >  1:237917/1‑110 (MQ=255)
                                                                                   gTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGaaa                                                       <  1:225969/148‑1 (MQ=255)
                                                                                    tCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGt                                                     >  2:296708/1‑149 (MQ=255)
                                                                                    tCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGt                                                     >  6:299938/1‑149 (MQ=255)
                                                                                      gACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGc                                                   <  5:192521/149‑1 (MQ=255)
                                                                                        cacCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCAc                                                 <  1:173296/149‑1 (MQ=255)
                                                                                          ccTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTg                                               <  5:110942/149‑1 (MQ=255)
                                                                                          ccTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTg                                               <  3:189803/149‑1 (MQ=255)
                                                                                              cTTCAAGCCCCTGCCCGGCTTCTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGtgc                                           <  8:294705/149‑1 (MQ=255)
                                                                                              cTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGtgc                                           >  5:133913/1‑149 (MQ=255)
                                                                                               ttCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGtgct                                          >  5:179420/1‑149 (MQ=255)
                                                                                                 cAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGtgctg                                         <  4:291976/148‑1 (MQ=255)
                                                                                                 cAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGtgct                                          <  6:133913/147‑1 (MQ=255)
                                                                                                 cAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGtgct                                          <  2:249892/147‑1 (MQ=255)
                                                                                                  aaGCCCCTGCCCGGCTACTACTTCACTTGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCAGCGCTGAAGTGGAAAGTGCACTGGTGCTGCa                                       <  5:96477/149‑1 (MQ=255)
                                                                                                  aaGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGtgctgc                                        <  4:223668/148‑1 (MQ=255)
                                                                                                  aaGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCa                                       >  4:44947/1‑149 (MQ=255)
                                                                                                   aGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCAt                                      <  8:205079/149‑1 (MQ=255)
                                                                                                       ccTGCACGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACa                                  <  6:321376/149‑1 (MQ=255)
                                                                                                             cGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTCAATGACAGCGTgg                            >  4:265340/1‑149 (MQ=255)
                                                                                                              ggCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGc                           >  5:146625/1‑149 (MQ=255)
                                                                                                               gCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGcc                          <  2:311031/149‑1 (MQ=255)
                                                                                                                ctactaCTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGcc                          >  2:131762/1‑148 (MQ=255)
                                                                                                                   ctactTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGGAAGTGCACTGGTGCTGCATGACAGCGTGGCCGaa                       <  4:147851/148‑1 (MQ=255)
                                                                                                                     actTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGcc                    >  7:221927/1‑149 (MQ=255)
                                                                                                                      ctTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCcg                   >  6:5169/1‑149 (MQ=255)
                                                                                                                                 aCGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGc        <  4:173682/149‑1 (MQ=255)
                                                                                                                                       cccGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCAGACGCCGCGGGGGGCGGCTccc    >  8:291989/1‑147 (MQ=255)
                                                                                                                                       cccGGCGCGAGGCCGACGGCGACTCCTGGCTCACCGGGCGCATCGACGAGGTGATCAACGTGTCCGGGCACCGCAGCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTAcc    <  7:291989/147‑1 (MQ=255)
                                                                                                                                        ccGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCAt                                                                           >  2:239263/1‑75 (MQ=255)
                                                                                                                                        ccGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTAcccc  >  1:252519/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                             
AAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCC  >  NC_002947/5345960‑5346243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑