Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,751	+G	intergenic (+113/+27)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,750	1	.	G	100.0%	102.6 / NA	30	intergenic (+112/+28)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/19);  total (11/19)

AGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674607‑5674888                                                                                                                                                 |                                                                                                                                           aGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggcc                                                                                                                                        >  1:155308/1‑145 (MQ=255)    gcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggccccc                                                                                                                                     >  5:124056/1‑142 (MQ=255)          cgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGtt                                                                                                                                <  1:31232/148‑1 (MQ=255)                tgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCa                                                                                                                            <  7:84746/146‑1 (MQ=255)                     cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCa                                                                                                                     <  2:127622/148‑1 (MQ=255)                         aGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGaaa                                                                                                                 <  3:11299/148‑1 (MQ=255)                         aGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGaaa                                                                                                                 <  6:124056/148‑1 (MQ=255)                          gggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAAt                                                                                                                <  5:110321/148‑1 (MQ=255)                           ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCGATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAAtt                                                                                                               <  5:119466/148‑1 (MQ=255)                              tGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACt                                                                                                            >  7:45923/1‑148 (MQ=255)                                   gAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                       >  2:85590/1‑148 (MQ=255)                                           gAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                               >  1:168140/1‑148 (MQ=255)                                             tagggCTGCGAAGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                             >  3:37991/3‑148 (MQ=37)                                                ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                          <  3:86453/148‑1 (MQ=255)                                                      ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                     >  8:6080/1‑147 (MQ=255)                                                                tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtc                                                                          >  1:119233/1‑148 (MQ=255)                                                                  tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        >  7:57468/1‑148 (MQ=255)                                                                               ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt                                                           <  6:58512/148‑1 (MQ=255)                                                                               ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAT‑CAGGTCCAGGGGCTGCGAAGTa                                                          <  6:32663/148‑1 (MQ=38)                                                                                                           tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCATTTGATGCCGAAGTCg                               >  6:2553/1‑148 (MQ=255)                                                                                                            aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  6:130804/143‑1 (MQ=255)                                                                                                            aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                <  3:129759/146‑1 (MQ=255)                                                                                                                gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                          >  8:59192/1‑148 (MQ=255)                                                                                                                gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                          >  6:23289/1‑148 (MQ=255)                                                                                                                   cGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                       <  5:23289/148‑1 (MQ=255)                                                                                                                   cGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                       <  7:59192/148‑1 (MQ=255)                                                                                                                            gtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt              <  4:136967/148‑1 (MQ=255)                                                                                                                                tGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          <  7:6080/148‑1 (MQ=255)                                                                                                                                 gATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg         >  7:34062/1‑148 (MQ=255)                                                                                                                                        ccGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  4:126386/148‑1 (MQ=255)                                                                                                                                        ccGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  4:126389/148‑1 (MQ=255)                                                                                                                                        ccGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGGCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  8:128699/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                           AGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674607‑5674888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑