Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,647,927	G→A	Y265Y (TAC→TAT)	oprB‑II ←	carbohydrate‑selective porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,647,927	0	G	A	100.0%	114.6 / NA	34	Y265Y (TAC→TAT)	oprB‑II	carbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/15);  total (19/15)

TCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTC  >  NC_002947/1647784‑1648067                                                                                                                                                |                                                                                                                                             tCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTgtaata                                                                                                                                            <  1:107287/146‑1 (MQ=255)          aGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCAc                                                                                                                                 <  6:49072/148‑1 (MQ=255)                   aCCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGGTGCCGGCTTACCGTTGCTGTCCTTGATAACATCTTGTGCCTTGGCATTACTGTAATAGTACCCGGCACGATATTccc                                                                                                                        <  2:12849/148‑1 (MQ=255)                     ctgctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCt                                                                                                                      >  7:153499/1‑148 (MQ=255)                         tgctgGGCGCCAATCAACAAGCCGTGCTTGCTCGAACTGATGCGGTAGGCGGCGCCGCTGAGCGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGAACGATATTCCCAttt                                                                                                                    >  6:15030/1‑146 (MQ=255)                               gcgcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                            <  7:81617/148‑1 (MQ=255)                               gcgcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                            >  7:39153/1‑148 (MQ=255)                                      tCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACg                                                                                                     >  6:159571/1‑148 (MQ=255)                                        cacaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTg                                                                                                   >  8:93474/1‑148 (MQ=255)                                         acaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGCCCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTgg                                                                                                  >  4:10959/1‑148 (MQ=255)                                         acaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTgg                                                                                                  >  8:139803/1‑148 (MQ=255)                                                  gcttgctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATAc                                                                                         <  6:94626/148‑1 (MQ=255)                                                           aaCTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGaa                                                                                <  1:161069/148‑1 (MQ=255)                                                                 tgcGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCa                                                                           <  5:159571/147‑1 (MQ=255)                                                                           ggcgCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCAccc                                                                >  3:157094/1‑148 (MQ=255)                                                                                           gccggcTGACCGGTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGtgccgctgccgc                                                <  7:10961/148‑1 (MQ=255)                                                                                                 tGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCACCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCt                                          >  7:107607/1‑148 (MQ=255)                                                                                                    ccGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCtt                                         <  8:113857/146‑1 (MQ=255)                                                                                                     cGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGaa                                      >  1:33737/1‑148 (MQ=255)                                                                                                              ccTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGc                              <  4:8102/147‑1 (MQ=255)                                                                                                               cTTGAGAACATATTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCAATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGcc                             >  2:180144/1‑147 (MQ=255)                                                                                                                ttGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCgg                           >  6:110295/1‑148 (MQ=255)                                                                                                                    gaACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGAtt                        >  4:105678/1‑147 (MQ=255)                                                                                                                     aaCATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGACTTGGATACGTGGGGTCAATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTTCCGGAtt                        >  3:156584/1‑146 (MQ=255)                                                                                                                       cATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAg                    <  8:39153/148‑1 (MQ=255)                                                                                                                        aTCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAgg                   <  5:110295/148‑1 (MQ=255)                                                                                                                        aTCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCACTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAgg                   >  1:121401/1‑148 (MQ=255)                                                                                                                           ttGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGtt                 >  7:124598/1‑147 (MQ=255)                                                                                                                               gCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGaa             <  7:144254/147‑1 (MQ=255)                                                                                                                               gCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGaa             <  7:139803/147‑1 (MQ=255)                                                                                                                                  ttGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGt         >  5:51250/1‑148 (MQ=255)                                                                                                                                     gCATTACTGTAATAGTCGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTc       <  8:75369/147‑1 (MQ=255)                                                                                                                                        ttACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCCTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCt   <  8:153499/148‑1 (MQ=255)                                                                                                                                            tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTc  >  4:72089/1‑145 (MQ=255)                                                                                                                                            tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTc  >  4:132314/1‑145 (MQ=255)                                                                                                                                                |                                                                                                                                             TCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTC  >  NC_002947/1647784‑1648067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑