Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,941,574	C→T	A269V (GCG→GTG)	PP_2574 →	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,941,574	0	C	T	100.0%	59.8 / NA	19	A269V (GCG→GTG)	PP_2574	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

TCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATG  >  NC_002947/2941433‑2941690                                                                                                                                               |                                                                                                                     tCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTc                                                                                                                >  5:169320/1‑148 (MQ=255)   ttCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCgg                                                                                                              >  7:119846/1‑148 (MQ=255)       gTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGggct                                                                                                          <  4:93698/148‑1 (MQ=255)                   tcgaGATGGTGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTg                                                                                              >  4:67280/1‑148 (MQ=255)                       gaTGGTGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGtt                                                                                           >  6:137713/1‑147 (MQ=255)                            tGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGc                                                                                      <  8:119846/147‑1 (MQ=255)                                        tcggtcAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGa                                                                          <  7:121634/147‑1 (MQ=255)                                              aGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGATGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAAcc                                                                   >  8:148493/1‑148 (MQ=255)                                                    tCGGTGTTTCGTTGCTGGGAACCCGGCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGGCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCa                                                             <  5:10519/148‑1 (MQ=255)                                                                tGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCGGGTGGTGCTGTCGGGTGTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGa                                                 <  6:169320/148‑1 (MQ=255)                                                                tGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGa                                                 <  3:73546/148‑1 (MQ=255)                                                                             tCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCGGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGaa                                    <  5:114197/148‑1 (MQ=255)                                                                                gCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAaga                                  <  8:27515/147‑1 (MQ=255)                                                                                    tCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTgg                             >  5:147551/1‑148 (MQ=255)                                                                                        aaTACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGaa                         >  2:61286/1‑148 (MQ=255)                                                                                                tATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCt                   >  2:137260/1‑146 (MQ=255)                                                                                                   gACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGGCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTGGTCGGGAAGAGCTGGCGAAGTTCCTGAAGt              <  2:141392/148‑1 (MQ=255)                                                                                                      ggCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCACACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGCGCCGGCGAAGTTCCTGAAGTGa            >  7:6202/1‑147 (MQ=255)                                                                                                               tcggCAATG‑TCGAGCATTGCCGAGCCGGTGGTGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATg  >  1:63383/3‑148 (MQ=255)                                                                                                                                               |                                                                                                                     TCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAGGGTTTCGGTTTTTCGTTGCTGGTAACCCGTCCGCACTCGGAATACACCTATGACGGCCAACGCCTGGCAATGCTCGA‑CATTGCCGAGCCGGTGGCGCTGTCGGGGCTGGCTGCGGCGTGGTTGAAGCGAGTCCAGCTGACCAAACCGGCGCAGTTGTTCGTGGAGTTTTGTCGGGAAGAGCTGGCGAAGTTCCTGAAGTGAGCACGCCATG  >  NC_002947/2941433‑2941690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑