Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,635,368	C→A	A130S (GCC→TCC)	uvrY ←	BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,635,368	0	C	A	100.0%	95.4 / NA	29	A130S (GCC→TCC)	uvrY	BarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/17);  total (12/17)

GGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGG  >  NC_002947/4635228‑4635495                                                                                                                                             |                                                                                                                                gggCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGc                                                                                                                          >  7:116158/1‑148 (MQ=255)  ggCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCt                                                                                                                            <  4:55783/145‑1 (MQ=255)        agGCACAACTTGTCGGTGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCCGa                                                                                                                   <  4:43442/148‑1 (MQ=255)         ggCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGat                                                                                                                  <  7:29034/148‑1 (MQ=255)             caACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAg                                                                                                              >  1:127842/1‑148 (MQ=255)                      ggAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCg                                                                                                     >  2:45526/1‑148 (MQ=255)                            gatCTGCATTTTGTGGCAGCCGTCTATCATGTGGGCTTTCTGGATTTCCGGTTCGTTCAACGGGTGTAACGGAGACCCGTGCGGCTGGAACGGTTTCAGCGCGAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCaaa                                                                                                <  4:137561/147‑1 (MQ=255)                                 gCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTAGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAg                                                                                          >  3:100235/1‑148 (MQ=255)                                                      tcatcaGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTc                                                                     <  4:50794/148‑1 (MQ=255)                                                        atcaGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGt                                                                   >  3:23476/1‑148 (MQ=255)                                                            gggCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAg                                                               <  2:118742/148‑1 (MQ=255)                                                              gCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGc                                                             >  1:154686/1‑148 (MQ=255)                                                                       aTTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCAc                                                       <  2:127842/145‑1 (MQ=255)                                                                       aTTTCCCGTGCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAATGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCt                                                    <  4:117611/148‑1 (MQ=255)                                                                        tttCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCtt                                                   >  1:57492/1‑148 (MQ=255)                                                                        tttCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCtt                                                   <  1:56874/148‑1 (MQ=255)                                                                                 cGGACAACGCGGCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTa                                          <  3:134079/148‑1 (MQ=255)                                                                                   gACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGc                                        >  3:121708/1‑148 (MQ=255)                                                                                        ttgtccGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCa                                   <  2:41875/143‑1 (MQ=255)                                                                                           gTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAgcg                                >  3:138637/1‑148 (MQ=255)                                                                                                               gCTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCGGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCgtgt            <  3:26211/148‑1 (MQ=255)                                                                                                                cTGGAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGtgtg           <  3:157211/148‑1 (MQ=255)                                                                                                                cTGGAACGGTTTCAGCGCCAACTGCTGGGAGAGCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGtgtg           <  4:136541/148‑1 (MQ=255)                                                                                                                   gAACGGTTTCAGCGCCAGCTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTggg         >  3:113039/1‑147 (MQ=255)                                                                                                                   gAACGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCaa                                                                >  8:94029/1‑92 (MQ=255)                                                                                                                    aaCGGTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAg                                                               <  7:94029/92‑1 (MQ=255)                                                                                                                       ggTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACg    <  8:93620/148‑1 (MQ=255)                                                                                                                        gTTTCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACgg   <  8:116158/148‑1 (MQ=255)                                                                                                                         tttCAGCGCCAACTGCTGGGAGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACggg  >  2:91357/1‑148 (MQ=255)                                                                                                                                             |                                                                                                                                GGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGG  >  NC_002947/4635228‑4635495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑