Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,816	T→G	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	57.9 / NA	19	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/10);  total (9/10)

GTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT  >  NC_002947/4740685‑4740946                                                                                                                                    |                                                                                                                                   gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCtt                                                                                                                   >  5:33390/1‑148 (MQ=255)       gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGc                                                                                                             >  5:34491/1‑148 (MQ=255)        aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                            <  1:166537/148‑1 (MQ=255)                    cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                               <  7:116609/148‑1 (MQ=38)                     gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                               <  4:51812/148‑1 (MQ=255)                       cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAACGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAgg                                                                                             <  1:105321/148‑1 (MQ=255)                                acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCCCGAAGAGGCCGGCCCAg                                                                                    >  5:59891/1‑148 (MQ=255)                                                gcgACATCGTTGCCCTGAAACACCGCAAGTACGCTGCAGTACCCGAACGCAACACGGCTGCCCTCGGCCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTCCGCGGCCTTGCCCGCCAATAGGCCGGCACAGACACCACAAAACCTCa                                                                    <  6:59100/148‑1 (MQ=255)                                                gccaCATCGTTGCCCTGAAACACCGCAAGTACGCTCCCGACCCCCAACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTCGCGGCCTTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCa                                                                    <  6:56273/145‑1 (MQ=255)                                                ccgACAGCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGACCGCACGAAGGCTGCCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGCCCTCTGCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCa                                                                    <  4:76092/147‑1 (MQ=255)                                                               tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccg                                                     >  4:31387/1‑148 (MQ=255)                                                                 aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCACAGCCACCACAAAACCTCAGGTTTTCTccgcccgc                                                    >  3:137591/1‑147 (MQ=255)                                                                                gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTc                                     <  6:33390/147‑1 (MQ=255)                                                                                      gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                              <  4:65421/148‑1 (MQ=255)                                                                                                  aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTAGCGGCCTTGCCCCCGAAGAGGGCCGCACAGACACCACAAAACCACAGTTTTTATACCCCCCCACACCCCCCCCTTTAAAGGTATTAAAAGgcgcct                  >  4:3944/1‑146 (MQ=255)                                                                                                  aagaagGCTGCCCTCGGGCAGCC‑TTGTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGGCCGCGGCGAGGGCGGGCCCGCCCCCACAACACCGCAGGTTTTATCCGCCCGCTCACGCCGCCCGTGGCGGGGATTGAACGgggccg                  >  8:33049/1‑146 (MQ=255)                                                                                                        gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca            >  4:54393/1‑148 (MQ=255)                                                                                                        gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCCCGGCCTTTCCCGCGAAGAGGCCCGCACACACACCCCACAACATCAGGTTTTATCCGCCCCCTCCCGCAGCCCTGTCAGGGTATTGAACTGCGCATccacca            >  6:57819/1‑148 (MQ=255)                                                                                                                  ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt  <  3:31387/148‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   GTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT  >  NC_002947/4740685‑4740946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑