Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,964,546	C→A	G271V (GGT→GTT)	fleQ ←	transcriptional regulator FleQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,964,546	0	C	A	100.0%	57.1 / NA	20	G271V (GGT→GTT)	fleQ	transcriptional regulator FleQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/10);  total (10/10)

CGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGT  >  NC_002947/4964405‑4964688                                                                                                                                              |                                                                                                                                               cGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCAc                                                                                                                                            <  5:70887/146‑1 (MQ=255)                    tAGGGAACACGTTCAGGCGGTAGTACTGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTg                                                                                                                       <  4:89326/148‑1 (MQ=255)                       ggAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTgca                                                                                                                     >  5:160959/1‑147 (MQ=255)                       ggAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTgca                                                                                                                     <  5:30932/147‑1 (MQ=255)                              gtccAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCacac                                                                                                               >  4:50690/4‑146 (MQ=255)                                      ggTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCtt                                                                                                     <  2:150089/148‑1 (MQ=255)                                      ggTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCt                                                                                                      >  8:5020/1‑147 (MQ=255)                                              aGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGc                                                                                              <  7:55293/147‑1 (MQ=255)                                              aGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCa                                                                                             >  6:103526/1‑148 (MQ=255)                                                   ttCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCgg                                                                                         >  3:83526/1‑147 (MQ=255)                                                      gcgGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCaa                                                                                      <  2:80933/147‑1 (MQ=255)                                                         gAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGc                                                                                  <  7:25344/148‑1 (MQ=255)                                                                         aTCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTtcgt                                                                  <  5:103526/148‑1 (MQ=255)                                                                         aTCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTtcg                                                                   <  4:83526/147‑1 (MQ=255)                                                                                      ggTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGCCCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGt                                                     >  8:89665/1‑148 (MQ=255)                                                                                         tCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTAcc                                                  >  7:110837/1‑148 (MQ=255)                                                                                                                 gcACGTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGccc                          <  2:162635/148‑1 (MQ=255)                                                                                                                     gTCGATGCTTTGGGTCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCa                      >  5:40798/1‑148 (MQ=255)                                                                                                                                   tCTTGTTGCTAACCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGCTGGc        >  4:12551/1‑148 (MQ=255)                                                                                                                                           cTAACCACGCGCTCGAACGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATCTCGCCGATTTCGTCCAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCCGCACGGCTGGAGATGGCCCCgga  >  2:183083/1‑145 (MQ=38)                                                                                                                                              |                                                                                                                                               CGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGT  >  NC_002947/4964405‑4964688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑