| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 96.9% | 102.5 / ‑3.3 | 32 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (13/18); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTT > NC_002947/1932496‑1932772 | gcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTG‑cccgcg < 3:322736/149‑5 (MQ=255) tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAg < 8:339836/149‑1 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAggg > 3:99659/1‑149 (MQ=255) ttCGAAGAGGATCTGGACATTAGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAggg > 4:53263/1‑149 (MQ=255) aTCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGCCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGc < 5:112682/148‑1 (MQ=255) tCTGGACTTTCTAACGCGGCTTTGCTGGTCTCACTAAAATCGACTTAAAGGGGGGGGCCGTTTTGCGGCCGTTGGCGGGAAAGCCCGCTCATACAAAGTATTTGCGTCCCCTTTTGGTGG‑GGGCTTGCCCGCGAAGGGCTGCAAGGCa < 4:99659/148‑1 (MQ=255) ggACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGC‑GGGCTTGCCCGCGAATGGCTGGAAGGCAGccc > 4:77372/1‑149 (MQ=38) gACAGCGGCATTCCAGGTCCCACCAAAATAGCCGTAACCCCTGGGGCCGCTTTGCGGCCCTTCGCGGCCACGCCCGCTCCTACAAAGCATATGCGCCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGt < 3:259258/149‑1 (MQ=37) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCCCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTa > 3:227293/1‑149 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCAGCCCGCGGCGAGAAAGGGTATGGGGGGCCGGTAGGGGCGGGGGTGG‑CCGCGACGGGCGGGAAGGGGGCCGCAAATTTGGGGCAACGCGGGTCAg > 8:138019/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 2:63480/1‑149 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATcc > 5:322161/1‑148 (MQ=255) tAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 7:32853/149‑1 (MQ=255) cAGTAACCGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTg < 2:28251/149‑1 (MQ=255) cTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGc < 3:53263/149‑1 (MQ=255) cTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGGCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTg < 6:322161/148‑1 (MQ=255) cgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGagc > 5:328683/1‑138 (MQ=255) cgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTgg < 3:77372/149‑1 (MQ=255) gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGagca < 6:328683/138‑1 (MQ=255) gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGt > 7:98955/1‑149 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATc < 4:119023/129‑1 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 8:178711/1‑149 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGtt > 2:353511/1‑104 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGtt < 1:353511/104‑1 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc > 1:375279/1‑148 (MQ=255) gATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAACGGGCAGGGTGAGGGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca > 1:326893/1‑148 (MQ=38) tGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 7:178711/148‑1 (MQ=255) gTCCCGTGTAGGAGG‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg < 7:138019/149‑1 (MQ=255) tCCCCTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 4:227293/149‑1 (MQ=255) ccTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 5:329645/1‑134 (MQ=255) cTGTAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac < 6:329645/134‑1 (MQ=255) tccgAGC‑GGGCTTGCCCTCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAAGCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 8:104107/146‑1 (MQ=255) tAGGAGC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt < 2:375279/148‑1 (MQ=255) | GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGA‑TGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTT > NC_002947/1932496‑1932772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |