Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,921,460	G→A	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	100.0%	126.8 / NA	41	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/20);  total (21/20)

CCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCAT  >  NC_002947/2921332‑2921599                                                                                                                                 |                                                                                                                                            ccGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGCCACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGCCTTc                                                                                                                         >  7:190946/1‑149 (MQ=255)        cGACCACCTGAACGGGCGCGCTGGCGCTGCCAGCCTGGATGGCGGCAACGGCAATGACCCCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACg                                                                                                                  <  2:271924/149‑1 (MQ=255)        cGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACg                                                                                                                  <  7:182736/149‑1 (MQ=255)         gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggg                                                                                                                                         >  7:209229/1‑125 (MQ=255)         gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACg                                                                                                                  >  5:62374/1‑148 (MQ=255)         gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACAACCTGCAGGGCGGCGATGGTAACTACACAATGTTCGTCTACGTGTGTACAGACACACTGATCAGGGGGGACGGCAACGACATCCTCAActg                                                                                                                 >  8:273815/1‑147 (MQ=255)              ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                            >  3:123684/1‑149 (MQ=255)                   tcGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGt                                                                                                       <  3:188864/148‑1 (MQ=255)                   aCGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg                                                                                                         <  6:62374/147‑1 (MQ=255)                     gggggCGCTGGCGATGTCATGCTGGATGGCGGCAACGGCAATGACACCGTTCAGGGCGGCGATGGTAACGACACATTGTTCTGCGACGTTGTTACCTACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTaa                                                                                                     <  7:218976/149‑1 (MQ=255)                           gcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAc                                                                                               >  3:146152/1‑149 (MQ=255)                           gcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAc                                                                                               <  4:123684/149‑1 (MQ=255)                               gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg                                                                                           >  3:385718/1‑149 (MQ=255)                               gCGATGACAGCATGGATGGCGCCAACGGCAAAGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACTATACggtg                                                                                           >  2:2869/1‑149 (MQ=255)                                 gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCGTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCGGGTAACGATACggtgg                                                                                          >  5:117936/1‑148 (MQ=255)                                 gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtggt                                                                                         >  8:57837/1‑149 (MQ=255)                                       aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGCGGGCCGGCAACGACTTACTCAACGGTGCGGCAGGTAACGATACGGTGgtgggtgg                                                                                   >  1:283415/1‑149 (MQ=255)                                       aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACAGTGTTCGCCGACGTGGGTACCGCCACCCTCATCAGGGGGGCCGGCAAAGACTGCCTGAACGGTGCGGCACGTAACGATACGGTggtgggtg                                                                                    >  5:272174/1‑148 (MQ=255)                                        gCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGgtgggtgg                                                                                   >  5:75888/1‑148 (MQ=255)                                         ccTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACAGCCTGAGCAGGGGGGCCGGCAACGACTTCCTGAACGGTGGGGCAGGTAACGATACGGTGGTGGGTgggg                                                                                 <  1:2869/149‑1 (MQ=255)                                              aTGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg                                                                             >  4:66811/1‑148 (MQ=255)                                                  cggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACg                                                                                              <  1:362267/127‑1 (MQ=255)                                                  cggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACg                                                                                              >  2:362267/1‑127 (MQ=255)                                                   ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGAAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGCCCCGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGCTCGTGGGTGGGGCCGGTAACGa                                                                       >  2:143903/1‑149 (MQ=255)                                                     caacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCGTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCGGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGac                                                                      <  6:117936/148‑1 (MQ=255)                                                      aacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGaca                                                                     <  6:75888/148‑1 (MQ=255)                                                          gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                 >  8:216597/1‑148 (MQ=255)                                                             aTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                             <  4:385718/149‑1 (MQ=255)                                                              tGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCg                                                            <  3:66811/149‑1 (MQ=255)                                                                    ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCCACACCCTCATCAGCGCCGCCGGAAACGACTACCTCAACCGTGAGCCAGGTAACGATACGCTGCTGGGTGGGGCCGGTAACGACACCAAGAACGCCAcc                                                       >  4:125289/1‑148 (MQ=255)                                                                        ctgggCTGCTATGTTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCATGGGGGCCGGCAAAGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                    <  2:283415/145‑1 (MQ=255)                                                                             cggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAATGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAAc                                              >  8:200281/1‑148 (MQ=255)                                                                               gcgATGGTAACGACACATTGTTCGGCGAGGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAAAGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                           <  1:143903/149‑1 (MQ=255)                                                                                      tAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                           >  8:260376/1‑142 (MQ=255)                                                                                      tAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                           <  7:260376/142‑1 (MQ=255)                                                                                      tAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTGCCTCAACGCAGCGGCAGGTAACGATACGTTGGTGGGTGGGGCCGGTAACCACACCATGATACCCACCGATGGAAACGAtctg                                        >  5:377512/1‑145 (MQ=255)                                                                                       taCGACACCTTGTTCGGCGACGTGGGTACCGACACCCTGCTCAGGGGGGCCTGCAACGACTTCCTCATCGGTGAGGCTGGTAACGATACGGTGGTGTGGGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATgtgt                                       <  6:377512/144‑1 (MQ=255)                                                                                        aCGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa                                   <  7:216597/148‑1 (MQ=255)                                                                                          gACACATTGTTCGGCTACGTGGGTACCGAAACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCATGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGtt                                <  7:273815/149‑1 (MQ=255)                                                                                                        cGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg                  <  7:57837/149‑1 (MQ=255)                                                                                                                   ccGACACCCTGATCAGGGGGGCCGGCAATGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTg       <  7:200281/149‑1 (MQ=255)                                                                                                                          ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat  >  6:250011/1‑147 (MQ=255)                                                                                                                                 |                                                                                                                                            CCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCAT  >  NC_002947/2921332‑2921599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑