Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,298	C→T	intergenic (+535/+218)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,298	0	C	T	100.0%	89.5 / NA	27	intergenic (+535/+218)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

ACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTC‑C‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGT  >  NC_002947/4741160‑4741409                                                                                                                                               |                                                                                                                aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAgcg                                                                                                        <  2:199558/149‑1 (MQ=255)   gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTAGCCAgcggc                                                                                                      >  3:233228/1‑149 (MQ=255)             cGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                                            <  2:247860/149‑1 (MQ=255)                    ggCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCGTCGTGCTTGAGCAGCTTCTGCGAGTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGAGCCTTGCTCACgggc                                                                                       >  6:292347/1‑145 (MQ=255)                     gCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                                                    <  1:48740/149‑1 (MQ=255)                      cGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                                                    >  6:108146/1‑148 (MQ=255)                              aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                           <  3:22948/149‑1 (MQ=255)                              aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑CCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                                                             <  5:207800/147‑1 (MQ=255)                               cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                           <  5:385340/148‑1 (MQ=255)                                  ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCCCC‑T‑GCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                                       >  1:258559/1‑149 (MQ=255)                                   gTATGGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACCCCGCGATAGGGCtg                                                                      >  4:256994/1‑148 (MQ=255)                                   gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                                                        <  5:197624/147‑1 (MQ=255)                                     ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCCCCTTGATCACGGTCACACCGCGATAGGCCTTg                                                                     >  7:75561/1‑148 (MQ=255)                                       aGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTAc                                                                  >  4:384857/1‑149 (MQ=255)                                        ggTCCAGCTCTGGTCACCTTCGAAATTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCAT‑GCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACt                                                                 >  7:59415/1‑149 (MQ=255)                                         gTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                >  4:4606/1‑149 (MQ=255)                                         gTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                >  1:308482/1‑149 (MQ=255)                                          tCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                               <  7:260739/149‑1 (MQ=255)                                                    gTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGcc                                                      <  3:4606/148‑1 (MQ=255)                                                                                 gacttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc                         <  3:256994/142‑1 (MQ=255)                                                                                  acttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc                        <  8:59415/143‑1 (MQ=255)                                                                                     tgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcg                     >  2:131311/4‑149 (MQ=255)                                                                                      gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                      <  2:308482/145‑1 (MQ=255)                                                                                       caTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcg                     <  5:108146/146‑1 (MQ=255)                                                                                        aTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtcc                  >  4:241096/1‑149 (MQ=255)                                                                                          tCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCtt                >  1:106935/1‑149 (MQ=255)                                                                                                         acaGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGt  >  2:363213/3‑149 (MQ=255)                                                                                                                                               |                                                                                                                ACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTC‑C‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGT  >  NC_002947/4741160‑4741409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑