Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 499,203 A→G intergenic (+47/‑249) uhpA → / → PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947499,2030AG98.0% 169.2 / NA 51intergenic (+47/‑249)uhpA/PP_0411two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/29);  minor base T (0/1);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC‑‑GCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCC  >  NC_002947/499057‑499337
                                                                                                                                                   |                                                                                                                                        
cAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑ggg                                                                                                                                        >  8:14643/1‑149 (MQ=255)
 aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑ggg                                                                                                                                        <  4:172686/148‑1 (MQ=255)
 aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑ggg                                                                                                                                        >  3:172686/1‑148 (MQ=255)
  agtccATCTGCACCCTCTAGGCACGGCTGTTGCAGAAGTTGATAGTGATCTCGCTGGCGGATCTGGTGTTGTACGGCATGGTGCACAAGCTGATCTGATTGCGAGATTCGGGTGCCGGAAACTTGCCGAAGCGTATGTGGGAGC‑TGGc                                                                                                                                       <  8:81459/145‑1 (MQ=255)
  aGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCTTGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCa                                                                                                                                      <  1:181971/149‑1 (MQ=255)
  aGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGc                                                                                                                                       <  5:385015/148‑1 (MQ=255)
      cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGcc                                                                                                                                  >  4:32196/1‑149 (MQ=255)
        tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCATGccg                                                                                                                                 >  7:139923/1‑148 (MQ=255)
        tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAt                                                                                                                                     <  8:306078/144‑1 (MQ=255)
        tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAt                                                                                                                                     >  7:306078/1‑144 (MQ=255)
            cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGAGAGC‑GGGCATGCCCGCGa                                                                                                                             >  4:143099/1‑148 (MQ=255)
            cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGAGAGC‑GGGCATGCCCGCGa                                                                                                                             <  3:143099/148‑1 (MQ=255)
             aCCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAAc                                                                                                                           >  7:60639/1‑149 (MQ=255)
               ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACgg                                                                                                                         >  8:307496/1‑149 (MQ=255)
                cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACggg                                                                                                                        >  4:93370/1‑149 (MQ=255)
                cacaAAGCACGCCGGATGGAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATCGAGCACAAGCTGATCTGATTGCGCCATACCGGTGCCCGAAACTTGCCGAAGCCGATGTGTGAGC‑GGGCAATCCCGCGCACgg                                                                                                                         >  6:33851/1‑148 (MQ=255)
                     ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctg                                                                                                                    <  6:99370/148‑1 (MQ=255)
                      gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctggc                                                                                                                  <  8:60639/149‑1 (MQ=255)
                      gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctggc                                                                                                                  <  3:32196/149‑1 (MQ=255)
                       cACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACgggccgggcc                                                                                                                 >  8:180180/1‑149 (MQ=255)
                       cACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctggc                                                                                                                  <  6:149589/148‑1 (MQ=255)
                        aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCCTGCCCCCGCACCggccatggct                                                                                                                >  1:208431/1‑143 (MQ=255)
                        aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGgacctggcct                                                                                                                >  1:334113/1‑149 (MQ=255)
                         cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGgcctggcctg                                                                                                               <  1:309834/149‑1 (MQ=255)
                         cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGgcctggcctg                                                                                                               <  7:329927/149‑1 (MQ=255)
                             tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCctgctg                                                                                                            <  5:20478/148‑1 (MQ=255)
                               aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGAt                                                                                                          <  2:226581/148‑1 (MQ=255)
                                 gCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGGACGGGCCTGGCCGGCTGAtcgc                                                                                                       >  3:315896/1‑147 (MQ=255)
                                   aGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCGATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTa                                                                                                     <  7:38601/149‑1 (MQ=255)
                                    gAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAt                                                                                                    <  3:93370/149‑1 (MQ=255)
                                    gAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAt                                                                                                    <  7:14643/149‑1 (MQ=255)
                                        ttGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCt                                                                                                >  7:358561/1‑149 (MQ=255)
                                               tGAAGTCGCTGGCGTAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAAGTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg                                                                                          <  5:33851/148‑1 (MQ=255)
                                                  aCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                        <  5:390795/147‑1 (MQ=255)
                                                      gCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTc                                                                                  >  1:60497/1‑149 (MQ=255)
                                                        tGGCGGTTCTGGTGCAGTACGCCATGGAGCACAAGCTGTTCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgc                                                                                <  8:358561/149‑1 (MQ=255)
                                                          gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgcg                                                                               <  3:150238/148‑1 (MQ=255)
                                                          gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgc                                                                                <  1:144294/147‑1 (MQ=255)
                                                           cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCggg                                                                             <  2:334113/149‑1 (MQ=255)
                                                                      aaGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAATCCCGCGAAAGGGCCTGGCCTGCGGAACTATATGGCTGAGCCGGCCACTTAGCCGGAATGCCC‑‑GCTc                                                                >  1:12307/1‑149 (MQ=255)
                                                                       aGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGAATGCCC‑‑GCTcc                                                               <  2:60497/149‑1 (MQ=255)
                                                                        gTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC‑‑Gcgaag                                                              <  2:298081/149‑5 (MQ=255)
                                                                         tACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGc                                                                                                 >  2:156247/1‑115 (MQ=255)
                                                                         tACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGc                                                                                                 <  1:156247/115‑1 (MQ=255)
                                                                              cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC‑‑GCTCCCACAGGt                                                        >  2:61111/1‑149 (MQ=255)
                                                                                       caAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg                                                                                          >  8:333537/1‑108 (MQ=255)
                                                                                       caAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCCCTATGGCTGAGCCg                                                                                          <  7:333537/108‑1 (MQ=255)
                                                                                                ctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATTCCCGCGCACCGGCCGGGGCGGGGGATGAGTATTGCGGGGCCGGCCCCTTAGCGGGCAAGCCC‑‑CCGCCCACAGGGTTAGTTGTTgggtagc                                        >  2:113029/1‑142 (MQ=255)
                                                                                                           atccGGGGGCCCGAAACGTGCCGAAGCCTATGGGGGAGC‑GGGCAGGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC‑‑GCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCaa                           <  7:86218/146‑1 (MQ=38)
                                                                                                                ggTGCCCGAACTTTGCCTACCCTTTTGTGGGTGC‑GGGCTTGCCCGTTAAGGGGCCTGGCTTGTTTATCTCTTTGGCTGTGCGGGCGTCTTCGGGGGATGGCCC‑‑GCTCCAACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg                      <  2:12307/149‑1 (MQ=255)
                                                                                                                      cGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAATCCCCCGAACGGGCCAGGCCTGCAGAAATCAATGGCTGAGCCGGCCACATAGCGGGCCAGCCC‑‑CCTCCCACACGTTTAGTGGTTGGCGACCAACGGCATTACAATACCCCCaac                 >  2:302222/1‑146 (MQ=255)
                                                                                                                              gCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC‑‑GCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAg          <  7:307496/147‑1 (MQ=255)
                                                                                                                                ctacgCCTATGTGGCAGC‑GGCCAGGCCCGCTACGGGGCCGGGCCTGCTGATCTCTATGGCTGAGCCGGCCCCTGCGCGGGCAGGCCCCAGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAggg        <  1:113029/145‑1 (MQ=255)
                                                                                                                                      cTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC‑‑GCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATcc  >  5:289674/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC‑‑GCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCC  >  NC_002947/499057‑499337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑