mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ALE-45_S45_L001_R1_001.good.fq | 398,065 | 57,383,283 | 100.0% | 144.2 bases | 149 bases | 94.3% |
| errors | ALE-45_S45_L001_R2_001.good.fq | 398,065 | 57,211,236 | 100.0% | 143.7 bases | 149 bases | 94.4% |
| errors | ALE-45_S45_L002_R1_001.good.fq | 389,079 | 56,020,107 | 100.0% | 144.0 bases | 149 bases | 95.6% |
| errors | ALE-45_S45_L002_R2_001.good.fq | 389,079 | 55,888,887 | 100.0% | 143.6 bases | 149 bases | 94.8% |
| errors | ALE-45_S45_L003_R1_001.good.fq | 398,263 | 56,899,171 | 100.0% | 142.9 bases | 148 bases | 95.5% |
| errors | ALE-45_S45_L003_R2_001.good.fq | 398,263 | 56,748,181 | 100.0% | 142.5 bases | 148 bases | 95.5% |
| errors | ALE-45_S45_L004_R1_001.good.fq | 372,611 | 53,628,326 | 100.0% | 143.9 bases | 149 bases | 95.4% |
| errors | ALE-45_S45_L004_R2_001.good.fq | 372,611 | 53,508,298 | 100.0% | 143.6 bases | 149 bases | 94.5% |
| total | 3,116,036 | 447,287,489 | 100.0% | 143.5 bases | 149 bases | 95.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 69.5 | 2.7 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 57860 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 747 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.037 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.80035 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 06:19:37 18 Feb 2020 | 06:20:23 18 Feb 2020 | 46 seconds |
| Read alignment to reference genome | 06:20:24 18 Feb 2020 | 06:27:16 18 Feb 2020 | 6 minutes 52 seconds |
| Preprocessing alignments for candidate junction identification | 06:27:16 18 Feb 2020 | 06:28:01 18 Feb 2020 | 45 seconds |
| Preliminary analysis of coverage distribution | 06:28:01 18 Feb 2020 | 06:29:48 18 Feb 2020 | 1 minute 47 seconds |
| Identifying junction candidates | 06:29:48 18 Feb 2020 | 06:36:34 18 Feb 2020 | 6 minutes 46 seconds |
| Re-alignment to junction candidates | 06:36:34 18 Feb 2020 | 06:38:52 18 Feb 2020 | 2 minutes 18 seconds |
| Resolving best read alignments | 06:38:52 18 Feb 2020 | 06:40:01 18 Feb 2020 | 1 minute 9 seconds |
| Creating BAM files | 06:40:01 18 Feb 2020 | 06:41:41 18 Feb 2020 | 1 minute 40 seconds |
| Tabulating error counts | 06:41:41 18 Feb 2020 | 06:42:14 18 Feb 2020 | 33 seconds |
| Re-calibrating base error rates | 06:42:14 18 Feb 2020 | 06:42:17 18 Feb 2020 | 3 seconds |
| Examining read alignment evidence | 06:42:17 18 Feb 2020 | 06:48:45 18 Feb 2020 | 6 minutes 28 seconds |
| Polymorphism statistics | 06:48:45 18 Feb 2020 | 06:48:45 18 Feb 2020 | 0 seconds |
| Output | 06:48:45 18 Feb 2020 | 06:49:33 18 Feb 2020 | 48 seconds |
| Total | 29 minutes 55 seconds | ||
