breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 195,561 T→G L220R (CTC→CGC)  ispU → undecaprenyl pyrophosphate synthase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 668,742 C→G E99Q (GAA→CAA)  rsfS ← ribosomal silencing factor
JC 1,675,679 (AGCCGC)2→3 coding (682/1389 nt) pntB ← pyridine nucleotide transhydrogenase, beta subunit
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,513,351 C→A L104M (CTG→ATG)  nupC → nucleoside (except guanosine) transporter
JC JC 2,536,312 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (479‑484/510 nt) crr → glucose‑specific enzyme IIA component of PTS
RA 3,440,225 A→G C269R (TGC→CGC)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC 4,016,106 (GGGTCTG)1→2 coding (64/816 nt) ysgA ← putative carboxymethylenebutenolidase
MC JC 4,233,804 1557 bp→83 bp coding (47‑1603/1650 nt) pgi → glucosephosphate isomerase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978319 1979743 1425 65 [59] [60] 62 insB1–insA insB1, insA
* * ÷ NC_000913 2468994 2470731 1738 61 [59] [59] 72 [gtrB]–gtrS [gtrB], gtrS
* * ÷ NC_000913 3423783–3424539 3424539 1–757 61 [60] [60] 61 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3769394 3770253 860 64 [55] [58] 61 [yibV]–[yibH] [yibV], yibV, yibU, yibU, [yibH]
* * ÷ NC_000913 3796848 3800363 3516 61 [60] [60] 62 [waaC]–[waaY] [waaC], waaL, waaU, waaZ, [waaY]
* * ÷ NC_000913 3804080 3805242 1163 61 [60] [57] 63 [waaB]–[waaP] [waaB], waaS, [waaP]
* * ÷ NC_000913 4475703 4477235 1533 61 [57] [55] 61 yjgL SopA‑central‑domain‑like hexapeptide repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =5 (0.040)102 (0.880) 66/468 0.4 95.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 5 (0.040)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078055 (0.040)125 (1.080) 74/468 0.2 96.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 5 (0.040)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related