Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,233,804 1557 bp→GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT coding (47‑1603/1650 nt) pgi → glucosephosphate isomerase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4233804 4235360 1557 79 [0] [2] 76 pgi glucosephosphate isomerase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 42338030 (0.000)45 (0.620)
+GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT
37/326 0.6 98.6% coding (46/1650 nt) pgi glucosephosphate isomerase
?NC_000913 4235361 = 2 (0.020)coding (1604/1650 nt) pgi glucosephosphate isomerase

ATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCG  >  NC_000913/4235086‑4235641
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                        
gtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCg                                                                                                                                                                                                                                                                  <  1:79673‑M2/25‑1 (MQ=255)
        ttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAg                                                                                                                                                                                                                                                          <  1:957170‑M2/33‑1 (MQ=255)
        ttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGc                                                                                                                                                                                                                                                         <  1:820393‑M2/34‑1 (MQ=255)
                  aaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagtccctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGt                                                                                                                                                                                                                                               >  1:849647‑M2/258‑301 (MQ=255)
                    atgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGAGCGGTTa                                                                                                                                                                                                                                             >  1:615201‑M2/256‑301 (MQ=255)
                      gcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAat                                                                                                                                                                                                                                           >  1:973311‑M2/254‑301 (MQ=255)
                         tcactgcgctaatggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatcat                                                                                                                                                                                                                                        <  1:704740‑M2/51‑1 (MQ=255)
                              gcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCAtcgtcg                                                                                                                                                                                                                                   >  1:744404‑M2/246‑301 (MQ=255)
                              gcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcaaaattctcaaaatcagaagagtattgctaatgaaaaaaatcaatcaaacacagaccgctgcctggcaggcactactgcgtaggctggagctgcttcgaagatcctatactttctagaaaataggaacttcgaaatgcaggtagacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCGATAACACGTGGCGCGGTTAATCAacgtc                                                                                                                                                                                                                                    >  1:288977‑M2/246‑300 (MQ=255)
                               cgctaaaggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGc                                                                                                                                                                                                                                                         <  1:543620‑M2/34‑1 (MQ=255)
                                   aagggtttacactcaacattacgctaacgtcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcataccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttctaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATg                                                                                                                                                                                                                              <  1:774191‑M2/61‑1 (MQ=255)
                                     gggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatctatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAACGCGTGGCGCGGTTAATCATCGTCGTTATGTa                                                                                                                                                                                                                            >  1:365157‑M2/239‑301 (MQ=255)
                                      ggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAg                                                                                                                                                                                                                           >  1:554821‑M2/238‑301 (MQ=255)
                                        tttacactcaacattacgctaacggcactaaaaccatcacatttgtctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAgg                                                                                                                                                                                                                          <  1:590711‑M2/65‑1 (MQ=255)
                                                 aacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAgg                                                                                                                                                                                                                <  1:664321‑M2/75‑1 (MQ=255)
                                                       acgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCa                                                                                                                                                                                                          >  1:1027395‑M2/221‑301 (MQ=255)
                                                        cgctaacgacactaaaaccatcacatttttctgtaacttgagctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAc                                                                                                                                                                                                         <  1:384766‑M2/82‑1 (MQ=255)
                                                                gcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctattctctagaaagtataggaacttcgaactgcaggtcgacggatccccggaatGCTCGa                                                                                                                                                                                                                                                                                     >  2:354355‑M2/212‑217 (MQ=255)
                                                                       aaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAACCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                                                                                                           >  1:561157‑M2/205‑300 (MQ=255)
                                                                         ccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCg                                                                                                                                                                                        <  1:1043844‑M2/99‑1 (MQ=255)
                                                                               tgcttcATCCTGCTGCGGAAAATCACTCCGTTCCGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGTGCGTGAGCCTGAACATCTTCACCTTCGACCAGTAGGGCGTAGAACTTGGAAAACAACTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGTTAAAGAAATCAGCAGCCACGATAGCTCAGCCAATGGTCTGATTAACCGCTATAAAGCGTGGCACAGTTAATCATCGTCGATATGTAGGCCAGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                                                                                                                                   <  1:392722/295‑1 (MQ=255)
                                                                               attttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                                                                                                                    >  1:650709‑M2/197‑299 (MQ=255)
                                                                                aCTTCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACTAGTTGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGACATTAGCAGCTACGATAGCTCGAACAATGGTATGATTaa                                                                                                                                                                                                                                                                     >  2:392709/1‑217 (MQ=255)
                                                                                  ntttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCg                                                                                                                                                                                                                                                                  <  2:809764‑M2/25‑1 (MQ=255)
                                                                                   tttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                                                                                                                                                              >  1:250197‑M2/193‑301 (MQ=255)
                                                                                         tgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATaa                                                                                                                                                                                                                                                            <  2:433591‑M2/31‑1 (MQ=255)
                                                                                          nactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAg                                                                                                                                                                                                                                                          <  2:952005‑M2/33‑1 (MQ=255)
                                                                                                  ctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                                                                                                                <  1:502742‑M2/123‑1 (MQ=255)
                                                                                                      natcttccaaagtcacaattctcaaaatcagaagaggattgctaatgaaaaaaatcaatccgacgcagaccgctgcctggcaggcactacggtgtaggctggcgctgctgcgaagttcctatactttctagagaatagtaacttcgaactgcaggtcgacggagccccggaatGCGCGACCAATGGTCTGATTAACAGCTATAAAGCGTGGCGCGgtg                                                                                                                                                                                                                                              <  2:1030940‑M2/45‑2 (MQ=255)
                                                                                                          ntccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatc                                                                                                                                                                                                                                          <  2:727395‑M2/49‑1 (MQ=255)
                                                                                                            ccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgcttcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatcat                                                                                                                                                                                                                                        <  2:441170‑M2/51‑1 (MQ=255)
                                                                                                            ccaaagtcacaattctcaaaagcaaatgagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcattacgttgtaggcttgagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATTGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                                                                                                                     <  1:975360‑M2/134‑1 (MQ=255)
                                                                                                                         tatcaaaagcagaacagtattgctaatgaaaaacaccataccaaccaagtccgctgccgggcaggcacgacggtgtaggctggagctccttcgaagttcctatactttctcgagaatagtaccttcgaactgcaggtcgacgtatccccgtaatGCTCGACAAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCCACCGATGCCTGCTGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                                                                                                        <  1:608808‑M2/147‑1 (MQ=255)
                                                                                                                              aaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggctggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                                                                                                                   <  1:744822‑M2/152‑1 (MQ=255)
                                                                                                                                  cagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATCTGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                                                                                                                      >  1:266053‑M2/146‑278 (MQ=255)
                                                                                                                                     aaaagtattgcgaatgaaaaacatcaatccaacgcagaccgctgcctggagggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                              <  1:229164‑M2/157‑1 (MQ=255)
                                                                                                                                                  atgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                                                                                                                  >  2:415736‑M2/130‑218 (MQ=255)
                                                                                                                                                        aacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctattctctagaaagtataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                         <  1:354367‑M2/178‑1 (MQ=255)
                                                                                                                                                          catcgatccaacgcagaccgctgcctggcaggcacgacggtgtaggctggagctgcttcgaagttccgatactttctagagaataggaacttagaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAAGCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                       <  1:415750‑M2/180‑1 (MQ=255)
                                                                                                                                                               atccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa                                                                                                   >  1:38568‑M2/117‑300 (MQ=255)
                                                                                                                                                                          accgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                                                                                                           <  2:250191‑M2/112‑1 (MQ=255)
                                                                                                                                                                                         gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTATGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTtag                                                                                                                                                           >  2:544917‑M2/91‑217 (MQ=255)
                                                                                                                                                                                         gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                                                                                                                                                              <  2:799387‑M2/125‑1 (MQ=255)
                                                                                                                                                                                         gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                                                                                                                <  1:544935‑M2/139‑1 (MQ=255)
                                                                                                                                                                                          cactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                          <  2:437436‑M2/129‑1 (MQ=255)
                                                                                                                                                                                          cactacggtgtaggctggagctgcttcgaaggtcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATGAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                          <  2:330798‑M2/129‑1 (MQ=255)
                                                                                                                                                                                              acggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgagctgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                                                                                                                      <  2:266046‑M2/133‑1 (MQ=255)
                                                                                                                                                                                                 gtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                                                                                                                   >  2:306838‑M2/83‑218 (MQ=255)
                                                                                                                                                                                                 gtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACg                                                                >  1:1032584‑M2/83‑301 (MQ=255)
                                                                                                                                                                                                    taggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                                                                                                                <  2:365145‑M2/139‑1 (MQ=255)
                                                                                                                                                                                                              ctgcttcgaagttcctatactttctagagaataggaacttcgaactgcagggcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                                                                                                        <  2:841011‑M2/147‑1 (MQ=255)
                                                                                                                                                                                                                      aagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTAt                                            <  1:415552‑M2/239‑1 (MQ=255)
                                                                                                                                                                                                                      aagttcctatacgttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                              <  2:615179‑M2/157‑1 (MQ=255)
                                                                                                                                                                                                                        nttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                            <  2:796262‑M2/159‑1 (MQ=255)
                                                                                                                                                                                                                         ttcctatactttctagagaataggaacttcgaacggcaggtcgacggatccccggaatGCTCGACCAAGGGTCTGATTAACCGCTATAACGCGGGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                            <  2:452494‑M2/159‑1 (MQ=255)
                                                                                                                                                                                                                                          gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGc                                                                                                                                                                                                                                                         >  2:106589‑M2/42‑75 (MQ=255)
                                                                                                                                                                                                                                          gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGc                                                                                                                                                                                                                                                         <  1:106593‑M2/34‑1 (MQ=255)
                                                                                                                                                                                                                                          gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa                                                                                                          >  2:7424‑M2/42‑218 (MQ=255)
                                                                                                                                                                                                                                          gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATcc                                 <  1:7424‑M2/250‑1 (MQ=255)
                                                                                                                                                                                                                                           nataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACAAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                         <  2:973263‑M2/178‑1 (MQ=255)
                                                                                                                                                                                                                                           aataggactgtcgcactgcaggtcgacggatactggggatGCTCGACCACTTGTCTGATTAACCGCTATAACGCGTGGCGCGGTTAATCATGGTCGTTATGTATGCCGGATAAGGCGTTCACGCCGCATCCGGTAACCGATGCCTGATGCGACGCGGTCTCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                         <  2:606603‑M2/178‑1 (MQ=255)
                                                                                                                                                                                                                                             taggaacttcgaactgcagggcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                       <  2:650686‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                                                             aaggaacttcgaactgcaggtcgacggatccccggaatGCTCGACAAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAAt                    <  1:848101‑M2/263‑1 (MQ=255)
                                                                                                                                                                                                                                                gaccttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCGGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg                                                                                                     <  2:288970‑M2/182‑1 (MQ=255)
                                                                                                                                                                                                                                                 nacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa                                                                                                   <  2:744376‑M2/184‑1 (MQ=255)
                                                                                                                                                                                                                                                  ncttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAc                                                                                                  <  2:1027333‑M2/185‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAAc                                                                                                                                                                                                                                                                    >  2:628076‑M2/32‑54 (MQ=255)
                                                                                                                                                                                                                                                    ttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAAc                                                                                                                                                                                                                                                                    <  1:628099‑M2/23‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACga                                                                                                <  2:38568‑M2/187‑1 (MQ=255)
                                                                                                                                                                                                                                                     tcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat                                                                                               <  2:554803‑M2/188‑1 (MQ=255)
                                                                                                                                                                                                                                                       naactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattg                                                                                             <  2:849606‑M2/190‑1 (MQ=255)
                                                                                                                                                                                                                                                          ctgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAcgattgatn                                                                                          >  2:869385‑M2/26‑217 (MQ=255)
                                                                                                                                                                                                                                                             caggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGCTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGCTGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATtaan                                                                                       >  2:743309‑M2/23‑217 (MQ=255)
                                                                                                                                                                                                                                                             aaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATAc    <  1:306846‑M2/279‑1 (MQ=255)
                                                                                                                                                                                                                                                                gtcgacggatgcccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAAt                                                                                      <  2:775301‑M2/197‑1 (MQ=255)
                                                                                                                                                                                                                                                                gtcgacggagccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGAGGCGGTTAATCATCGTCGATAAGTAGGCCGGATAAGGCGTTCACGCCGCAGCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCg  <  1:743337‑M2/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                        
ATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCG  >  NC_000913/4235086‑4235641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: