Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,875,5210TC23.8% 113.3 / 15.1 65A28A (GCT→GCCyidXputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base C (15/0);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 4.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAA  >  NC_000913/3875274‑3875808
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                               
gtaCAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGa                                                                                                                                                                                                                                            >  1:60306/1‑301 (MQ=255)
       cTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTTTCCCATACCGCAAAGGTTAGTTCAGGACAGCATCAATCGCAGTCCGACACCAt                                                                                                                                                                                                                                      >  1:271328/1‑300 (MQ=255)
       cTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTCCTTTACAGAAAAAGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCAt                                                                                                                                                                                                                                      >  1:976609/1‑300 (MQ=255)
             ttgttgTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTCTGCTTGACAGAAAAAAGTAGGTCAGGAAAGTATCAATCGCAGTCAGACACGATAACgggg                                                                                                                                                                                                                               >  1:523076/1‑301 (MQ=255)
               gttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGTTCTGCTATACAGCAAAGGGTAGTTCAGGAAAGTATCAATAGCAGTCAGACACATTCACTggg                                                                                                                                                                                                                               >  1:388590/1‑299 (MQ=255)
                  gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCCCTGGGCTAt                                                                                                                                                                                                                           >  1:194128/1‑300 (MQ=255)
                     ctacAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTGCCATACCGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGGCAGACACACTCACTGGGCTATGGCa                                                                                                                                                                                                                       >  1:189752/1‑301 (MQ=255)
                                    aaaaaagaaaaagGCAGAAAAAAATTACATGCGACGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAAAAGTAGGGAAAGAGGAATATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTaa                                                                                                                                                                                                        <  1:859224/300‑1 (MQ=255)
                                            aCAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTTTGCCATACCGCAAAAGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAATATAGTACTGGAACaa                                                                                                                                                                                                >  1:971920/1‑301 (MQ=255)
                                               aTGCATAAAAAAATGACATGCGTTAGGCAGAAGTCTGAAAAGGGATATGAGGCGCTAAAAATGAAGGAAAAAATTATATGCTTTTAACGGAATAGATACTCGATTAAAGAATTAATAATAAAGTTTAATTTTAAGGGATTTTTAACGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATAGAACAAAAg                                                                                                                                                                                             <  1:690854/301‑1 (MQ=255)
                                                 gCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAANGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTATAACACAAAGAGTAGTTCAGGAAAGTATCAATCGCATTCAGACACCATCACTGGGCTATCGCAGTCAAAAGATGGTAATTGAACAAACgg                                                                                                                                                                                            >  1:1016735/1‑300 (MQ=255)
                                                          aaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGtn                                                                                                                                                                                                                                                                     >  2:874473/1‑217 (MQ=255)
                                                           ntGACAGGCATCGGGCAGAAATCTGAAGAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCTCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTc                                                                                                                                                                                                                                                                    <  2:689714/217‑1 (MQ=255)
                                                             gACGTGCGGCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAAGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGt                                                                                                                                                                                <  1:827349/300‑1 (MQ=255)
                                                                  gCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTgg                                                                                                                                                                                                                                                                                             >  2:645753/1‑186 (MQ=255)
                                                                  gCGTCAGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGAAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTgg                                                                                                                                                                                                                                                                                             <  1:645776/186‑1 (MQ=255)
                                                                                  tggaataggatatcactttgtaaaaccgaggGAATGAAGTTCATGTTTTCAATTGCTTAACTATACTTTTAAAGTATTATTTATGAATTTAATTTTGAAAGCATATTTTAAGGCTGCCGATTTATTCCCACTGGCGCTGCTGCTTTCAGGCTGTATCTCGTATGCTCTAGTTTCCCGTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAAACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCtt                                                                                                                                                          <  1:101566/274‑1 (MQ=255)
                                                                                    aaaaGGGATATCAGGGGCGAAACAGGAGGGAAAGAAGAGTATGCGTTCAACGGCTTAGCTACTAGTTTAAAGGATTAATCATGAAGTTGAATTATAAGGGATATTTTAAGGCTGAAGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTAcc                                                                                                                                                            <  1:600774/297‑1 (MQ=255)
                                                                                        gagtagacAGGCGCTGAACGGGAGGGAACGGAGAGTATGCTTTCAACGGCTTAACTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCGGCCGGTTTATTCCCCCTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCACATACCGAAAAGGGTAGTTAAGGAAAGTATCAATCGCAGTCAGACACAATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAc                                                                                                                                                     <  1:450111/293‑1 (MQ=255)
                                                                                             atCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTGCCATACCGCAAAGCGTAGTTCAGGAAAGTATCAATCGCAGTCCGACACCATCCATGGCCTATAGCAGGAAACAGATAGTGATGGAACAAAAGGCTATGTTTTTGTCGGGGAGTCGCTTGAATCCCATATCACTGATgg                                                                                                                                               >  1:879692/1‑301 (MQ=255)
                                                                                                  gcgcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTGCTATACCGCAAAGGGTAGTTCAGGAAAGTATCAATc                                                                                                                                                                                                                                                     >  2:304956/1‑194 (MQ=255)
                                                                                                  gcgcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTggg                                                                                                                                                                                                                               >  2:450095/1‑216 (MQ=255)
                                                                                                  gcgcTAAACAAGAGGGAAATAAGAATATGCTGTCAACGGATTAGCTACGCGTTTAGAAGATTAATTATGGAGATGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGATTTCAGTCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATc                                                                                                                                                                                                                                                     <  1:304964/194‑1 (MQ=255)
                                                                                                              aggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTCGTCTCCTATAACGCAAAGGATAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACACACGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGttt                                                                                                                              >  1:44845/1‑300 (MQ=255)
                                                                                                                ggAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTCCCAGAAAGCAAAAGGTAGTTAAGGAAAGTATCAATCGAAGTTACACACCCTCACTGTGCTAACAGATGAAAAAGTTAGCAATGGCACAACAGGCCAAGTTTTTGCATGGGAATAACTGTCTTACCTTATTACTTATggatcagaacgcatagtttaa                                                                                                                            >  1:755078/1‑282 (MQ=255)
                                                                                                                 gAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGGAGTTCAGGAAAGTATCAATCGCAGTCAGACACAATAACTGGGCTATCGCAGGCAAAAgn                                                                                                                                                                                                              >  2:767969/1‑217 (MQ=255)
                                                                                                                 gAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACAGCAAAGGGTAGTTCAGGAAAGTATCAATCTCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATTGCTGGATTACCTTATCACTGATGGTGCCGATGAATTCGTTAAGa                                                                                                                           >  1:355613/1‑301 (MQ=255)
                                                                                                                 gAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTCTCCCATACCACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAACGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAATGATGGTGCCGATGACATCGTTAAGa                                                                                                                           >  1:431065/1‑301 (MQ=255)
                                                                                                                 gAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCATATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAGCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAgn                                                                                                                                                                                                              >  2:731884/1‑217 (MQ=255)
                                                                                                                  aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTCTGCCATACCGCAAAGGGTAGTTAAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGAAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTCGGGGAATCGCCGGATTACCTTATCACTGATGGTGGCGATGACATCGTTAAGAt                                                                                                                          >  1:725444/1‑301 (MQ=255)
                                                                                                                  aaaGAAGAGTATGCTTCCAACGGCGTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGAt                                                                                                                                                                                                             <  2:422975/218‑1 (MQ=255)
                                                                                                                         agTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGTTTTCCCATACAAANAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGCACAAAAGGCTATGTTTTTTTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGAACTGGTTAAGATGCTCa                                                                                                                     >  1:1011566/1‑299 (MQ=255)
                                                                                                                           tatacgttCAACTGTTGATCTACTCGAATAAAGGATTAATCAGGTAGTTGAATTTTAAGGGATTTTGTAAGGCTTCCAATATATTCCCACTGGCGCTAATACTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCAGACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGa                                                                                                                 <  1:400890/295‑1 (MQ=255)
                                                                                                                              gCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGaa                                                                                                                                                                                                   >  2:794191/1‑216 (MQ=255)
                                                                                                                                    naatgCTTAGATGTTCGATTAAAGGATTAATCGTGAAATTGAATTTGAAGGTATTTTTTAAGTCTGCCGGTTTATGCCCACTGGCGCTGATGCGTTCAGGCTGTAGCTCGTATGCTCTGGTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAATGATAGTGATTGAACAAAAggg                                                                                                                                                                                           <  2:1030907/214‑2 (MQ=255)
                                                                                                                                       ggCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCACAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTAt                                                                                                                                                                                        >  2:246974/1‑218 (MQ=255)
                                                                                                                                       ggCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTCTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTaa                                                                                                     >  1:559924/1‑301 (MQ=255)
                                                                                                                                         cTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCGTCTGCTTTACCGAAAAGGGTAGTTCACGAAAGTATTAATCGTAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATCGAACAAAAGGCCATTTTTTTGTAGGGGAATCGCTGGATTACCTTATTACTGATGCTGACGATTATATCGTTAATATGCTCAATGTTCCAGctacttcc                                                                                                    >  1:64674/1‑293 (MQ=255)
                                                                                                                                          ttAGCTAGTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtt                                                                                                                                                                                     <  2:125126/218‑1 (MQ=255)
                                                                                                                                              atACTCGTTTAAAGGATTAATCAAGAAGTTGAATTTGAAGGGATTTTTTAAGGCTGCCGGTTTATTACCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTAAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTg                                                                                                                                                                                 <  2:194124/217‑1 (MQ=255)
                                                                                                                                                   cGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAatat                                                                                         >  1:758252/1‑301 (MQ=255)
                                                                                                                                                   cGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAg                                                                                                                                                                                                           >  2:627901/1‑187 (MQ=255)
                                                                                                                                                   cGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAg                                                                                                                                                                                                           <  1:627924/187‑1 (MQ=255)
                                                                                                                                                   cGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTCTGCCATACCACTAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATAGTTAAGATGCTCAATGAACCAGCACTTAACAGGCACAatat                                                                                         >  1:768991/1‑301 (MQ=255)
                                                                                                                                                        aaaaGATGAATCATGAAGTTGAATTTTAAGGGATTGTTTAAGGCTGTCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGAAAAGGGTAGTTCAGAAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATTACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAgg                                                                                    <  1:146013/301‑1 (MQ=255)
                                                                                                                                                           ggATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGttt                                                                                 >  1:191150/1‑300 (MQ=255)
                                                                                                                                                            taTTAATCGAGAAATTGAAGTTTAAGGTATGTTATAAGGCGGCCGTTTTATTCCCACGAGCACTGATGCTTTCAGGCTATATCTAGTAAGCTCTGGTTTCCAATACCGCAAAGGGTAGTACAGAAAAGTATCAAACACAGACAGACACCATCAATGGGCTATCGCAGGAAAAAGATAGTAATGAAACAAAAGGCTATGTAGTTGTAGGGGAATCACTGGATTAACTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAgg                                                                                    <  1:972116/296‑1 (MQ=255)
                                                                                                                                                            gATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCACAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCgatn                                                                                                                                                                   >  2:972068/1‑215 (MQ=255)
                                                                                                                                                                         gTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTTTGCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACAGGCACACTATTCAGGTTTCCGATGACGCACGa                                                                   >  1:604507/1‑301 (MQ=255)
                                                                                                                                                                                           ttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTCTGCCTGACAAAAAAAGAAAGTTCAGGAAAATTACAATCGCACTCATACACCATCAACGTGCTATCGCAGGCAAAAGATAGTATTGGAAAAAAATGCTCTGTTTTTCTAGTGGAATCGCTGGATTACCTTATCACAGATGGTGCCGATGACATCGGCAAGATCCTCAATGTTCGAACACTTCACCGCTACAACCTTTAGTTTGCCAATGACACACGAtgacttttcatgaggtga                                                 >  1:729204/1‑284 (MQ=255)
                                                                                                                                                                                                      ccGGTGTATTCCCACTTGCGCTGATGCTTTCAGGCTGGATCTCGTATGCGCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGAAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGa                                                                                                                           <  2:971872/216‑1 (MQ=255)
                                                                                                                                                                                                        ggTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCTTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCACTGATCCACCACTTAACCGGCACAATATTAAGGTTGCAGATGACGCAAGATGTGTTTTAAATGCGGGTAAAAAGAACTTTa                                    >  1:251132/1‑301 (MQ=255)
                                                                                                                                                                                                                    cTGTCGCTGATGCTTTTAGGCTGTATCTTGTATGCTCTGGTTTCACATACCGCAAAGGGTAGTTCAGGAAAGTATCCATCGCAGCCAGACACCATTGGTGGGCGATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTGAACAGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAatat                        <  1:874518/301‑1 (MQ=255)
                                                                                                                                                                                                                       gcgcTGATGCTTGCAGGCTGTATCTCATATGCTCTGGTTACCCATAACGCAAAGGGTAGTTCAGGAAAGTATCAATCACAGTCAGACACCATCACGGGGCTATGGCAGGCAAAAGATAGTAAGGGAACAAAAGACTATATTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGGTCCAGCAc                                                                                                         <  2:60305/217‑1 (MQ=255)
                                                                                                                                                                                                                        tggtGATGCTGTCGTGCTTTATCTAGGATGCTGTGTTTTCGCATACCGCAAAGGGTATTTCATGAAAGTATCAATAGCAGTCAGATATCATCACGGGGCTATGGCAGGCAAAAGAGAGTAATGTAACAAAAGGATATATTGTTGGAGGGGAATCGCTGGATTACCTTATCACTGATTGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCat                                                                                                         <  2:1011504/213‑2 (MQ=255)
                                                                                                                                                                                                                             aTGATTTCAGGCTGGATATCGTATGCTCTGGATTACAATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGAAACCATCACTGGGCTAGCGCAGGCAAAAGATAGTAATGTAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAAc                                                                                                    <  2:1016673/216‑1 (MQ=255)
                                                                                                                                                                                                                             aTGATTTCAAGCGGAATCTCTGATGCTATGGTTTCCCATACCGCAAGGGGTAGTTCGGGAAAGTATCAATCGCATTCAGAGACCATCACTGGGCTATCATATGCAAAAGTTATTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAAc                                                                                                    <  2:1003847/216‑1 (MQ=255)
                                                                                                                                                                                                                                cTTTCAGGCTGTATCTCGTATGCCGTGGTTTGCCTTAACACAAAGGGTAGTTCAGGAAAGTATCAATCCCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAACAGGCTACTCTTTTGTTGGGGAATCGCTGGATTACCTTATCACTGATGTTGCCGATGACACCGTTAAGATGCTCAATGATTCATCACTTAACCGGTACAATATTTCAGTTGCCGATGACGCACGACTTGATTTAAATGCTGGGAAAAAGAACTCTATCTGCACAACACCCCTTtacta              >  1:281522/1‑299 (MQ=255)
                                                                                                                                                                                                                                cTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAACTGCGGGGAAAAAGAAATTTACCGGCACACTATCGCTTtacta              >  1:320723/1‑299 (MQ=255)
                                                                                                                                                                                                                                      ggCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACGGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAatan                                                                                         >  2:730845/1‑217 (MQ=255)
                                                                                                                                                                                                                                       gCTGTAGCTCGTATGCTCTTGTTTCCCATACCGCAAAGGGTATTTCAGGAAAGTATCAATCGGAGTCAGACACCATCTCTGTGCTAGCGCAGGCAAAGGAGAGTAATGTAACAAAAGGCTATGTTTTTGTAGGGGAATCGATGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTATCCGGCACAata                                                                                          <  2:431051/216‑1 (MQ=255)
                                                                                                                                                                                                                                        cTGTATCTCGTATTCTCTGGTTTCCCATACCGGAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGaa     <  1:730873/300‑1 (MQ=255)
                                                                                                                                                                                                                                          gTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAg                                                                                     >  2:547348/1‑218 (MQ=255)
                                                                                                                                                                                                                                           tATCTAGGATGCGCTGGGTTCCCATACCGCAAAGGGTAGTTAAGGAAAGTATGAATCGCAGTCAGACACCATCACGGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGGAGGGGAATCGCTGGATTACCTGATCACGGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATaa  <  1:820093/300‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGAt                                                                            >  2:217164/1‑218 (MQ=255)
                                                                                                                                                                                                                                                      ctctGGTTTCCCATACCGCAAAGGGTAGTTCAGGCAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAACAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGGTAAGATGCTCAATGATCCAGCACTTCACCGGCACGATATTCAGGTGGCCGATGa                                                                          >  2:84206/1‑217 (MQ=255)
                                                                                                                                                                                                                                                      ctctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGa                                                                          >  2:332736/1‑217 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                               
GTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAA  >  NC_000913/3875274‑3875808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: