Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,183,274 A→G N677S (AAC→AGC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,183,2740AG98.7% 766.7 / NA 224N677S (AAC→AGC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (85/136);  minor base T (2/1);  total (87/137)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

CGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACC  >  NC_000913/4182978‑4183571
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         
cGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCAAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGACCACGATGACGCCAGCCGt                                                                                                                                                                                                                                                                                                       >  2:1341179/1‑301 (MQ=255)
      gACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCAt                                                                                                                                                                                                                                                                                                   <  2:1121103/299‑1 (MQ=255)
      gACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTg                                                                                                                                                                                                                                                                                                 >  2:59937/1‑301 (MQ=255)
       aCTAACGAATACGGCTTCCTTGAGACTCCGTAGCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCAt                                                                                                                                                                                                                                                                                                   <  2:53548/298‑1 (MQ=255)
        cTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTAAATGGACGTATCCACCAAGCAGGTGGTATCCGTCGGTGCGTCCCTGCTCCCGTTCATGGAACACGATGAACCCAGCCGTGCATTGag                                                                                                                                                                                                                                                                                               >  2:836933/1‑300 (MQ=255)
          aaCGAATACTGCTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCATGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGTAGACCTGGTAACGTGTCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATggg                                                                                                                                                                                                                                                                                            <  2:1152708/301‑1 (MQ=255)
                    gCTTCCTGGAGACTCCGTATCGTAAAGTTACCGACTGTTGTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAACCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACa                                                                                                                                                                                                                                                                                    <  2:1050753/300‑1 (MQ=255)
                        ccTTGAGACCCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCaa                                                                                                                                                                                                                                                                               >  2:961823/1‑301 (MQ=255)
                           tGATACTCCGTTTCGTAAAGTTGCCGACGGTGTTGTAAGTGACGAAATTCACTACCTGTCTGCTATCGAAGACGGCAACTACGTTATCGCCCAGGCTGACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCGTGTTCAGCCGCGACCGTGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGTTCCCTTTCCTGGAACACGATGCCGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGt                                                                                                                                                                                                                                                                            <  2:25117/301‑1 (MQ=255)
                             agaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTc                                                                                                                                                                                                                                                                           <  2:287418/300‑1 (MQ=255)
                             agaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTc                                                                                                                                                                                                                                                                           <  2:1002669/300‑1 (MQ=255)
                                cgccGCATCGTAAAGTGACCGACTGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTAGATGAAGAAGGCCACTTCGTAGAAGGCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                                                                                                                                                                                                                                       <  2:43687/299‑1 (MQ=255)
                                      aTCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGtta                                                                                                                                                                                                                                                                  >  2:840305/1‑299 (MQ=255)
                                            aaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATg                                                                                                                                                                                                                                                                                              >  2:569549/1‑266 (MQ=255)
                                                        gtgtTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACTCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCTGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGTTGCGAACATGCAACGCCAGGCCGTTCCGACTCTGCGCTCTGATat                                                                                                                                                                                                                                               >  2:1141489/1‑300 (MQ=255)
                                                           ttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGcc                                                                                                                                                                                                                                            <  2:232184/301‑1 (MQ=255)
                                                                 cTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                                                                                                                                                                                                                      <  2:135335/301‑1 (MQ=255)
                                                                            cccaacccgtctgcaagcgcaacagcaaaCCACTTTACCGCCCAAGCGAACTCCAAACTGCAGGAAGAAGGCCACTTCGGAGACGAACTGTTCACTTGCCGCATCAAAGGCGACTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGGGCGTCCCTGATCCCGTTCCTGTAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTa                                                                                                                                                                                                                           <  2:365155/274‑1 (MQ=255)
                                                                            cACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCTAACTCCAACTTGGATGAAGAAGGCCCCTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTa                                                                                                                                                                                                                           <  2:80711/301‑1 (MQ=255)
                                                                            cACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACTTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTgggg                                                                                                                                                                                                                           >  2:477177/1‑299 (MQ=255)
                                                                            cACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGAGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGCTGGGTGCGAACATGCCACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAATGGTa                                                                                                                                                                                                                           >  2:730408/1‑301 (MQ=255)
                                                                                 cttttCTGCTATCGATGAAGGCCACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCTCTTCGTAGAAGACCTCGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCATCCGCGACCAGGTTGACTACATGGACGTATCCACTCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGaa                                                                                                                                                                                                                      <  2:1241163/297‑1 (MQ=255)
                                                                                 ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACNCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGaa                                                                                                                                                                                                                      <  2:1408780/301‑1 (MQ=255)
                                                                                  cTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAAc                                                                                                                                                                                                                     <  2:900292/301‑1 (MQ=255)
                                                                                       tGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCAt                                                                                                                                                                                                                                                                                                   <  1:28768/218‑1 (MQ=255)
                                                                                         cTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTg                                                                                                                                                                                                                                                                                                 >  1:754325/1‑218 (MQ=255)
                                                                                         cTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc                                                                                                                                                                                                                                <  2:754339/283‑1 (MQ=255)
                                                                                              naagaatGCACCTAAGTTATCGCCCAGGCGAACTACATCTTTGATGAAGAAGGCCATTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGCCTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATggg                                                                                                                                                                                                                                                                                            <  1:1364129/217‑1 (MQ=255)
                                                                                                   aggctgcTACGTTCTTGCCCAGGCGAACTCCAACTTGGATGAAGATCGTCACTTCGTAGAAGACCTGGTACCTCGCCGTAGCAAACGCCAATCCAGCTTGTTCAGCCGTGACCAGGTTGACTACATGGACGTACCCACTCAGCAGGTGGTATCCGTCGCTGCGTCCCTTATCCCCTTCCTCGAACCCGATGACCCAATCCGTCCATTCATTGGTGCGa                                                                                                                                                                                                                                                                                       >  1:171995/7‑218 (MQ=255)
                                                                                                    ggCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGTAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGACCCCGTTCCTGGAACACGATTACGCCATCCGTGCATTGATGGGTGCGAACATGCATCGTCAGGCCGTTCCGACTCTGCCCGCTGATATGCCGCTGGCTGGTTCTGTTTTGGAACGTTCTGTTGCCGTTgttt                                                                                                                                                                                                   >  2:612065/1‑298 (MQ=255)
                                                                                                         cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGc                                                                                                                                                                                                                                                                                 <  1:23353/218‑1 (MQ=255)
                                                                                                         cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCggg                                                                                                                                                                                              >  2:777454/1‑300 (MQ=255)
                                                                                                           aCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGgtgt                                                                                                                                                                                            <  2:553750/301‑1 (MQ=255)
                                                                                                             gTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACAGGCacan                                                                                                                                                                                                                                                                             >  1:1339727/1‑215 (MQ=255)
                                                                                                                   gCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCgg                                                                                                                                                                                    >  2:1281841/1‑301 (MQ=255)
                                                                                                                    cccAGGCGAACTCCAACTTTGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCTAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGt                                                                                                                                                                                   <  2:150429/301‑1 (MQ=255)
                                                                                                                            naCTCCAACTTGGATGGAGAAGGCCACTTCGTAGAAGACCGGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGAGCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAc                                                                                                                                                                                                                                                              <  1:1283720/217‑1 (MQ=255)
                                                                                                                                          aaagaaagGCCATTCGTAGAAGACCTGGTAAACTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTc                                                                                                                                                            <  2:500134/296‑1 (MQ=255)
                                                                                                                                            aagaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTc                                                                                                                                                            <  2:1212711/300‑1 (MQ=255)
                                                                                                                                               aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGc                                                                                                                                                                                                                                             <  1:533415/216‑1 (MQ=255)
                                                                                                                                                      cTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAAATGCGGTAGCTAAACGTGGTGGTGTCGGTCAGTACGTGGAt                                                                                                                                                 >  2:264896/1‑301 (MQ=255)
                                                                                                                                                        tCGTAGAAGATCTGGTAACTTGCCGTAGCAAAGGCGAATCCATCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTTCTGGAACACGATGACGCCAGCCGTGCATTGATCGTTGCGAACATGCATCGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                                                                                                                                                                                                                  >  1:436539/1‑218 (MQ=255)
                                                                                                                                                        tCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGCTGACGCCCGCCGTGCATTGATGGGTGCGATCCTGCAACGTCAGGCCGTTCCTACTCTGCTCGCTGATAAGCCGCtggctggt                                                                                                                                                                                                                                  >  1:836563/1‑218 (MQ=255)
                                                                                                                                                        tCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGa                                                                                                                                                  >  2:917148/1‑298 (MQ=255)
                                                                                                                                                        tCGGAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGc                                                                                                                                               <  2:793746/301‑1 (MQ=255)
                                                                                                                                                          ntAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc                                                                                                                                                                                                                                <  1:1183625/217‑1 (MQ=255)
                                                                                                                                                          gTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATACAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGCCGTATCCACCCAGCAGGTGGTATACGTCGGTGCGTCCCTGATCCCGGTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTan                                                                                                                                                                                                                                >  1:1129433/1‑217 (MQ=255)
                                                                                                                                                               ntaCCTGGTAAACTGCCGCAGCAAAGGCTAATCCAGCTTGTGCAGCCGCGACCAGGTTGCCGACATGGACGTCTCCACCCAGCAGGTGGTATCCGTCTGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGACCTTGCAACGTCAGGCCGTTCCGTCTCTGCGCGCTGATAAGCCGCTGGTTGGTTCTGGTa                                                                                                                                                                                                                           <  1:1237784/216‑1 (MQ=255)
                                                                                                                                                               aaaCCGGGTAACTTGCCGTAGCAAAGGCGAAGCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCGGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTa                                                                                                                                                                                                                           <  1:473790/216‑1 (MQ=255)
                                                                                                                                                                 gccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGCCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTa                                                                                                                                                                                                                           <  1:1049574/215‑1 (MQ=255)
                                                                                                                                                                  ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTCACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGGTGCTTCCCGTAt                                                                                                                                      >  2:324288/1‑300 (MQ=255)
                                                                                                                                                                   cTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCg                                                                                                                                    <  2:852437/301‑1 (MQ=255)
                                                                                                                                                                     ggTAACTTGTCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTa                                                                                                                                                                              >  2:971338/1‑257 (MQ=255)
                                                                                                                                                                      gTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGaa                                                                                                                                                                                                                      <  1:1001867/216‑1 (MQ=255)
                                                                                                                                                                            tGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGaa                                                                                                                                                                                                                      <  2:141782/210‑1 (MQ=255)
                                                                                                                                                                             gCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAg                                                                                                                           <  2:623874/300‑1 (MQ=255)
                                                                                                                                                                                 tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCg                                                                                                                                                                                                         >  1:87637/1‑218 (MQ=255)
                                                                                                                                                                                        ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGTTGCTTCCCGTATCGTTATCAAAGTTAACGCAGACg                                                                                                               >  2:672709/1‑301 (MQ=255)
                                                                                                                                                                                         gCGAATACAGCTGGTCAAGCCGGGACCAGGTTAACTCAATTGACGTATACGCCAAGCAGGTGGTATCCGTCGGTGAGTCCATGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACt                                                                                                                                                                                                   <  1:924805/216‑1 (MQ=255)
                                                                                                                                                                                            aaTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGATCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGCACACGCAACGGCAGGCCGTTCCGCCTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGCACGTGCTGTTGTCGTCGACTCCGGTGTAACTGAGGTAGCGAAACGTGGTGGTGTCGTTCCGTACGTGGCTGCTCCCCGTATCGTTATGAAAGTTCACGAAGACgcgag                                                                                                           >  2:405394/1‑300 (MQ=255)
                                                                                                                                                                                                               cgACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTaaa                                                                                                                                                                            >  1:619783/1‑217 (MQ=255)
                                                                                                                                                                                                                 aCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTa                                                                                       <  2:296909/300‑1 (MQ=255)
                                                                                                                                                                                                                   cAGGTTGACTACATGGGCGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTTCAACTTGATGACGCCAGCCGTGCATTCATGGGTGCGACCATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACg                                                                                                                                                                          <  1:518544/215‑1 (MQ=255)
                                                                                                                                                                                                                    aGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCTGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTTCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTCCTGGTATGGAACGTGCTGTTGCCGTTGACCCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAATGTTAACGAAGCCGAGATGTATCCGGGTGAAGCAGGTAt                                                                                      >  2:651293/1‑298 (MQ=255)
                                                                                                                                                                                                                         gACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTTATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGgtgt                                                                                                                                                                 >  1:943253/1‑218 (MQ=255)
                                                                                                                                                                                                                         aaCTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTCTCGACAt                                                                                >  2:165704/2‑299 (MQ=255)
                                                                                                                                                                                                                             aCATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACaa                                                                          >  2:150686/1‑301 (MQ=255)
                                                                                                                                                                                                                             aCATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAAAGAAAACGAGATGTAGCGGGGTGAAGCAGGTATCGAAATATACaa                                                                          >  2:808777/1‑301 (MQ=255)
                                                                                                                                                                                                                             aCATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGCGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACaa                                                                          >  2:1009946/1‑301 (MQ=255)
                                                                                                                                                                                                                                tggtgttctcctccCAGCACGTGGTATCCGTCGGTGCGTCCCTTATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAg                                                                                                                                                          <  1:202164/210‑1 (MQ=255)
                                                                                                                                                                                                                                  gaagTATCCACCCAACAGGTGGGATCCGGCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCGGTGCAGTGATGGGTGAGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGAATCCGGTGTAACTGCAGTAGCTAAACGTGGTGGTGTCGTTCAg                                                                                                                                                          <  1:966437/213‑1 (MQ=255)
                                                                                                                                                                                                                                   ncGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTAc                                                                                                                                                       <  1:961799/217‑1 (MQ=255)
                                                                                                                                                                                                                                   aCGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTAc                                                                                                                                                       <  1:150486/218‑1 (MQ=255)
                                                                                                                                                                                                                                      tATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGtn                                                                                                                                                    >  1:1403247/1‑217 (MQ=255)
                                                                                                                                                                                                                                        tCCACCCAGCAGGTGGTATCCGGCTGTGCGTCCCTGATCCCGTTCCTTGAACACGATGACGCCAGACGTGCATTGATGGGTGCTAACATGCAACGTCAGGCTGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGa                                                                                                                                                  <  1:629466/218‑1 (MQ=255)
                                                                                                                                                                                                                                          cACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATg                                                                                                                                                <  1:14078/218‑1 (MQ=255)
                                                                                                                                                                                                                                          cACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATa                                                              >  2:1311573/1‑300 (MQ=255)
                                                                                                                                                                                                                                            nccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCt                                                                                                                                              <  1:1109485/217‑1 (MQ=255)
                                                                                                                                                                                                                                            cccAGCAGGTGGTATCTGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGACAGCCGTGCATTGATGGGGGCGAACAGGCAACGTCAGGACGTTCCGAATCTGCGCTCTGATAAGCGGCTGGTTGGTACTTGTATGGNCCGTACTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGTGAAACGTGGTAGTGTCGTTCAGTACGTGGTGGCTTCCCGCACCGTTATCAAATGTCACGAATACGCAAGATATACGTGTGAAGCAGGTATGTCCTGCTACAACGTGAACAAATacaa                                                           >  2:1399717/1‑300 (MQ=255)
                                                                                                                                                                                                                                                gcagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg                                                        <  2:465036/300‑1 (MQ=255)
                                                                                                                                                                                                                                                gaagGTGGTACCCGACGGTGCGGCCATGAGCGCGTTCCTGAAACACGATGACGGCAGCCGTGCATGGATGGCTGGGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACAcc                                                          <  2:310498/296‑1 (MQ=255)
                                                                                                                                                                                                                                                    gtggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTAt                                                                                                                                      >  1:299523/1‑218 (MQ=255)
                                                                                                                                                                                                                                                       ccttCCGTCGGTCCGTCCTGTATCCCGTTCCTGGAACACGAGGAGGCCCTCCTTGCATTGATGGGTGTGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGTTTAGCCGCTGGTTGGTACTGGTATGGACCGGGCTGTTGCCGTTGATTCCGGTGTAAATGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCg                                                                                                                                    <  1:568286/214‑1 (MQ=255)
                                                                                                                                                                                                                                                           ccGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACAAAATACACCCGTTCTAACCAGaa                                            >  2:212443/1‑301 (MQ=255)
                                                                                                                                                                                                                                                              tcggtcggTCCCTGATCCCGTTCCTTGAACACGATGACGCCAGCCGTTCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAacac                                         <  2:680428/301‑1 (MQ=255)
                                                                                                                                                                                                                                                               cGGTGCGTCCCTGATCCCGTTCATGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAg                                                                                                                           <  1:399563/218‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cttCCCTGCTCCCGTGCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTAt                                   <  2:1338575/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTAt                                   <  2:386909/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cccTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATCCACCAGTTCTAACCAGAACGCCTGTATCaa                                >  2:528377/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                         cTGTTCCCGTTCCTTGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTTCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc                              <  2:943277/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cTGATCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc                              <  2:448200/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cTGATCCCGTTCCTGGAACACGAGGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc                              <  2:1042572/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cTGATCCCGTGCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTACGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc                              <  2:1199310/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aTCCCGTTCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTNCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTAc                                                                            >  2:1403968/1‑252 (MQ=255)
                                                                                                                                                                                                                                                                                  ttCCTAGAACACGGTGACGCCAGCCGTGCATTAGTGGGTGCGAACATGCAACGTCAGGCCGTTCCGGCTCTGCGCGCTGGTAGTCCGCTGGTTGGTACTGTTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAATACGAGATGTa                                                                                                        <  1:368127/218‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   tCCTGGAACACGATTACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg                    <  2:1144223/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   tCCTGGAACACGATGACGCCAGCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc                                                >  2:118920/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                                    ccTGGAACACGATGACGCCAGCCGTGCATTGGTGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATc                                                                                                      <  1:575024/218‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           cacGATGAGGCTAGGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCGGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTGCTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctt            <  2:171995/301‑2 (MQ=255)
                                                                                                                                                                                                                                                                                                     ccAGCCTTGCTTTGATGCGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCACAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAcc  <  2:512700/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         
CGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACC  >  NC_000913/4182978‑4183571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: