Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,233,804 1557 bp→GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT coding (47‑1603/1650 nt) pgi → glucosephosphate isomerase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4233804 4235360 1557 73 [0] [0] 65 pgi glucosephosphate isomerase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 42338030 (0.000)40 (0.600)
+GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT
30/332 1.0 100% coding (46/1650 nt) pgi glucosephosphate isomerase
?NC_000913 4235361 = 0 (0.000)coding (1604/1650 nt) pgi glucosephosphate isomerase

GGTTGAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGT  >  NC_000913/4235074‑4235647
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                              
ccactcctggcggtaagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacaatacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGt                                                                                                                                                                                                                                                                                   <  2:103608‑M2/14‑1 (MQ=255)
                     tcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCg                                                                                                                                                                                                                                                              <  2:589528‑M2/35‑1 (MQ=255)
                           aataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAAtggtctgattaaccgcgccacgctttatagc                                                                                                                                                                                                                                                        <  2:334404‑M2/41‑16 (MQ=255)
                                   cttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCACGGTTAatc                                                                                                                                                                                                                                                <  2:623171‑M2/49‑1 (MQ=255)
                                                  ggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgtcggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGGTATGTAg                                                                                                                                                                                                                                 >  2:459284‑M2/238‑301 (MQ=255)
                                                  ggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagacagctgcctggcaggcactacggtgtaggctggagctgctccgaagttcctatactttctagaaaataggaacttcgaactgcaggtagacggatccccggaatGCACGACCAATGGTCTGATTAACCGATATAAAGCGTGGCGCCGTTAATCATCTTCGTTATGtaa                                                                                                                                                                                                                                 >  2:432159‑M2/238‑300 (MQ=255)
                                                          cgcaacattacgctaacggcactaaaaccaacacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaagcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCAGGATa                                                                                                                                                                                                                         <  2:724035‑M2/72‑1 (MQ=255)
                                                              acattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgcactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATCTGTAGGCCGGATAAgg                                                                                                                                                                                                                      >  2:929177‑M2/226‑300 (MQ=255)
                                                                               ctaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                                                                                    >  2:206362‑M2/209‑301 (MQ=255)
                                                                                       atcacattattctgtgactggcgctacaatcgtccaaagtcacaattctcaaaatcagaatagtattgctaatgaaaaacatcaatccaacgctgcccgctgcctggcaggcaatactgtgtaggcaggagctgcttcgaagttcctatactttctagagaataggaacttcgaactggaggtcgacggatccccggaatGCTCGACCAATGGTCTGa                                                                                                                                                                                                                                                                               <  1:429501‑M2/18‑1 (MQ=255)
                                                                                                           gcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTgg                                                                                                                                                                                                                                                           >  1:191543‑M2/181‑218 (MQ=255)
                                                                                                                   accttccaaagtcacaattctcaaaatcagaagagtattgctaatgacaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                <  2:336393‑M2/129‑1 (MQ=255)
                                                                                                                       cacacagtcacaattctcaaaatcagacgagtatttctatgtataaccatcactccaacgcgaaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatgctttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATTGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                                                                                                                            <  2:209323‑M2/133‑1 (MQ=255)
                                                                                                                             gtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATTTAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCAACCTATGCCTTATGCCACGCGGTCGCGTCTTCTCAGGCCTACAg                                                                                                                                                      >  2:217639‑M2/163‑301 (MQ=255)
                                                                                                                                   nttctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAGGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                   <  1:926972‑M2/62‑1 (MQ=255)
                                                                                                                                   attctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                   <  1:230224‑M2/62‑1 (MQ=255)
                                                                                                                                     tctcaacatcagaagcgtattgctaatgcaaaacatcactcctcccccgaccgctgcatggccggcacttctgtgtaggctggagctgattcgaagttccgatactttctagagaataggatcttcgaactgcaggtcgacggatccccggaatGCTCGACCACTGTTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGCTATGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                                                                                                              <  2:326839‑M2/147‑1 (MQ=255)
                                                                                                                                     tctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                                                                                                              <  2:191546‑M2/147‑1 (MQ=255)
                                                                                                                                       tcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGc                                                                                                                                                                                                                               <  1:121182‑M2/66‑1 (MQ=255)
                                                                                                                                           aatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGa                                                                                                                                                                                                                           >  1:19091‑M2/149‑218 (MQ=255)
                                                                                                                                                 aagtgtatgactaatgaacaacatccatccaacgcagcccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                  <  2:928220‑M2/159‑1 (MQ=255)
                                                                                                                                                 aagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgtcggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAgg                                                                                                                                                                                                                      <  1:459281‑M2/75‑1 (MQ=255)
                                                                                                                                                 aagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                    <  2:816250‑M2/157‑1 (MQ=255)
                                                                                                                                                 aagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                  <  2:19091‑M2/159‑1 (MQ=255)
                                                                                                                                                 aagagtattgctaatgaaaaacagcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                    <  2:668813‑M2/157‑1 (MQ=255)
                                                                                                                                                      taatgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatatgaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAt                                                                                                                             <  2:800727‑M2/164‑1 (MQ=255)
                                                                                                                                                           ctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                        <  2:651860‑M2/169‑1 (MQ=255)
                                                                                                                                                                 aaaaacatcaagccaacgcagaccgctgccgggcaggcactacggtgcagtctggagatgctgcgcagttcctatactttcaagagaataggtagttcgaaccgcaggtcgacgcagccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTGAATCATCGTGGATATGGAGGTCGGATAAGGCGTCCACGCCGCCTc                                                                                                                                                                                                       <  1:410435‑M2/90‑1 (MQ=255)
                                                                                                                                                                      tatcaatccagcgcagaccgctgcttggcatgcaccacgtttggcgctggagttgcttagaagttattatacttgttatagaataggaacttcgaactgcaggtcgacggatccccgtaatGCTCGACCAATGGTCTGATTAACCGCTAAAAAGAGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                             <  2:662543‑M2/180‑1 (MQ=255)
                                                                                                                                                                      catcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                                                                                                                 >  1:490116‑M2/122‑217 (MQ=255)
                                                                                                                                                                      catcaagccaacgcagaccgctgccgggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttatagagaataggaacgtcgaacggcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                        <  2:490119‑M2/169‑1 (MQ=255)
                                                                                                                                                                           atccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGgtaattacttcct                                                                                                             >  2:766784‑M2/117‑290 (MQ=255)
                                                                                                                                                                           atccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCCATTAATACATAGCa                                                                                                         >  2:140856‑M2/117‑300 (MQ=255)
                                                                                                                                                                              caacgcagaccgctgcctggcaggcacgacggtgtaggctggagatgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACAAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat                                                                                                     <  2:281744‑M2/188‑1 (MQ=255)
                                                                                                                                                                              caacgcagaccgctgcctggcaggcaatacgaggtaggcgtgagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat                                                                                                     <  2:490032‑M2/188‑1 (MQ=255)
                                                                                                                                                                                acgcagaccgcgtcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattg                                                                                                   <  2:453300‑M2/190‑1 (MQ=255)
                                                                                                                                                                                             cctggcaggcactacggtgtaggctggagctgcttcgaagttcctatacttcttagagaataggaacttcgaactgaaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCt                                                                                       <  2:799815‑M2/202‑1 (MQ=255)
                                                                                                                                                                                                 gcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagcaacttcgaactgcaggtcgacggatccccggaatGCTCTACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                                                                                                                      <  1:439316‑M2/123‑1 (MQ=255)
                                                                                                                                                                                                  caggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                                                                                                                      <  1:640405‑M2/123‑1 (MQ=255)
                                                                                                                                                                                                    ggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAAc                                                                                <  2:767705‑M2/209‑1 (MQ=255)
                                                                                                                                                                                                             gtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAc                                                                                                        >  2:407767‑M2/83‑267 (MQ=255)
                                                                                                                                                                                                                                cgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGcc                                                                                                                                                                                                                              <  2:171355‑M2/67‑1 (MQ=255)
                                                                                                                                                                                                                                cgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGcc                                                                                                                                                                                                                              >  1:171354‑M2/64‑130 (MQ=255)
                                                                                                                                                                                                                                  aagttcctatacttactcgtgaaaaggaacttcgaactgcagttcgacgaatcctcggaatTCTCAACCAATGGTCTGATTAGCGGCTATAAAGCGTGGCTCGTTTAATCATGGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTATTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACTATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATc                                                 <  2:294811‑M2/240‑1 (MQ=255)
                                                                                                                                                                                                                                    nttcctatactttctagagaataggaacttcgaactgcaggtcgagggagccccggaatGCTCGACCATGGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                  <  1:856603‑M2/159‑1 (MQ=255)
                                                                                                                                                                                                                                     ttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACatn                                                                                                                                 >  1:922085‑M2/59‑217 (MQ=255)
                                                                                                                                                                                                                                     ttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                  >  1:518470‑M2/59‑217 (MQ=255)
                                                                                                                                                                                                                                          atactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTatn                                                                                                                            >  1:830551‑M2/54‑217 (MQ=255)
                                                                                                                                                                                                                                          atactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                             <  2:830578‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                                                              nttctagggaataggaccttcgaacggaaggtcgacggatcccaggaatGCTCGACCAGTGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                        <  1:726333‑M2/169‑1 (MQ=255)
                                                                                                                                                                                                                                              nttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                        <  1:646616‑M2/169‑1 (MQ=255)
                                                                                                                                                                                                                                               ttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                                                                                                                         >  1:914091‑M2/49‑216 (MQ=255)
                                                                                                                                                                                                                                                      gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                             >  2:572204‑M2/42‑221 (MQ=255)
                                                                                                                                                                                                                                                      gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAAATAGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTg                                  >  2:51038‑M2/42‑296 (MQ=255)
                                                                                                                                                                                                                                                      gaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCcccttgctgtt                             >  2:195256‑M2/42‑296 (MQ=255)
                                                                                                                                                                                                                                                         taggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                             <  1:572195‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                                                                         taggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                             <  1:382613‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                                                                            gaaagtcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                             <  1:766760‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                                                                              acttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAc                                                                                                        <  1:407764‑M2/185‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aaggtcgacggatccacggaatGATGTGCCAATGGGCTGATTAACCGCGATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCNACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATAc          <  2:922124‑M2/279‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAAt                                                                                            <  1:206359‑M2/197‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa                                                                                           <  1:161081‑M2/198‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGt  <  2:140971‑M2/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    ntggcaggcatGCTCACCCAATGGTCTGATTAACCTGTATAATGCTTGGCGCGGTTAATCATCGTCTATAGGTAGGCCGGATAAGGCGTTCACGCAGCATCCGGCAACCGATTCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGTAAATTAAGACATAACACGATTGATTAAATAACCttaatc                                                                                  <  1:783078‑M2/207‑2 (MQ=255)
                                                                                                                                                                                                                                                                                        ncggaatGCTGGACAAATGGTCGGATTAACCGCTATAAAGCGTGGCGCGGTTACTCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCAGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACaa                                                                              <  1:696101‑M2/211‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                              
GGTTGAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGT  >  NC_000913/4235074‑4235647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: