Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,202,957 G→T 5.4% intergenic (‑24/‑67) cohE ← / → croE e14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,202,9570GT 5.4% 405.8 / 8.2 137intergenic (‑24/‑67)cohE/croEe14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (62/67);  new base T (5/3);  total (67/70)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-01

Only 100 of 138 total aligned reads displayed.

CCATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGCTACTTAC  >  NC_000913/1202813‑1203105
                                                                                                                                                |                                                                                                                                                    
ccATAAACCTGCTGGTCTGGGTTGGCTCGCGATCTATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTaa                                                                                                                                                  <  1:2071127/149‑1 (MQ=255)
ccATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTaa                                                                                                                                                  >  2:570054/1‑149 (MQ=255)
ccATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTaa                                                                                                                                                  >  2:334896/1‑149 (MQ=255)
 cATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAg                                                                                                                                                 >  2:1617390/1‑149 (MQ=255)
 aaTAATCCTGCTGGTCTGGGTTGGCTCGCGATCAATGATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGTTGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAg                                                                                                                                                 <  1:1356848/148‑1 (MQ=255)
  aTAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTAGTACCGTTAAgg                                                                                                                                                >  2:717052/1‑149 (MQ=255)
    aaaCCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCTTAACGGTa                                                                                                                                              <  2:1987665/149‑1 (MQ=255)
      aCCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACa                                                                                                                                            >  2:2142373/1‑149 (MQ=255)
        ctgctgGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCTTAACGGTACAAg                                                                                                                                          <  2:669574/149‑1 (MQ=255)
         tgctgGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGt                                                                                                                                         >  2:1258317/1‑149 (MQ=255)
              gTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATATCGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCtt                                                                                                                                    >  1:1476279/1‑149 (MQ=255)
                cTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCAAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGaa                                                                                                                                 <  1:8068/149‑1 (MQ=255)
                  gggTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAg                                                                                                                                >  1:539042/1‑149 (MQ=255)
                      tGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTc                                                                                                                            <  1:1849211/149‑1 (MQ=255)
                       tgcgagcgATCAAGCATAGTGGCAAATGAAGAATTCCCGCCAACACCATCTCTAAGTTTTCTGGCGTTAGACCGACGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCa                                                                                                                           <  1:205976/144‑1 (MQ=255)
                        gCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCAt                                                                                                                          >  1:1217896/1‑149 (MQ=255)
                        gCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCAt                                                                                                                          >  2:479512/1‑149 (MQ=255)
                        gCTCGCGATCAATCATAGAGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCAt                                                                                                                          <  1:467269/149‑1 (MQ=255)
                          tCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCAttt                                                                                                                        >  1:554916/1‑149 (MQ=255)
                            gcgATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCa                                                                                                                      >  1:707359/1‑149 (MQ=255)
                              gATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACAGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGCACCGTGAAGGTAATAGTATCTTGAAGGTTCATTTCAAt                                                                                                                    >  2:1368559/1‑149 (MQ=255)
                               atcaatcaTAGTGGCAAAGGAAGAATTCCCGCCCACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATc                                                                                                                   >  2:1060413/1‑149 (MQ=255)
                               atcaatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATc                                                                                                                   >  1:1498289/1‑149 (MQ=255)
                                tcaatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCa                                                                                                                  >  1:699958/1‑149 (MQ=255)
                                 aaatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCAt                                                                                                                 <  2:1415565/148‑1 (MQ=255)
                                   atcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGt                                                                                                               >  1:2013635/1‑149 (MQ=255)
                                    tcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCTTGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTa                                                                                                              >  2:511621/1‑149 (MQ=255)
                                     caTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTaa                                                                                                             <  2:2047073/149‑1 (MQ=255)
                                      aTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCAGTTCAATCATGTAat                                                                                                            <  1:730516/149‑1 (MQ=255)
                                        cgTGGCAACGGAAGAATTCCCGCAAACACCATCTCTCCGTTTGCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTCAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAatat                                                                                                          <  1:2091476/148‑1 (MQ=255)
                                        aGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAatat                                                                                                          <  2:1744542/149‑1 (MQ=255)
                                        aGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAatat                                                                                                          <  2:626615/149‑1 (MQ=255)
                                          tGGCAAAGGAAGAATTCCCGCCAACACAATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCTATCATGTAATATGt                                                                                                        >  1:1613358/1‑149 (MQ=255)
                                           ggCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAAAGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAAAAATGTAATATGTa                                                                                                       >  1:1832451/1‑149 (MQ=255)
                                            gCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAACCCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTac                                                                                                      >  2:1845330/1‑149 (MQ=255)
                                            gCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTac                                                                                                      >  1:1182147/1‑149 (MQ=255)
                                              aaaGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTacac                                                                                                    >  2:2014663/1‑149 (MQ=255)
                                                aGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCg                                                                                                  <  2:852551/149‑1 (MQ=255)
                                                 ggAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCgg                                                                                                 <  2:890431/149‑1 (MQ=255)
                                                   aagaaATCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGGTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCTATTCAATCCTGTAATATGTCCACCCGAg                                                                                               <  2:1027134/149‑1 (MQ=255)
                                                        ttCCCGCCAACACCATCTCTCAGTATTCTGGCGTTAGACCGCCGGATGTCATGGAGTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGCAGGTACa                                                                                          <  1:1661081/149‑1 (MQ=255)
                                                            cGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAACACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATAtt                                                                                      <  1:1861882/149‑1 (MQ=255)
                                                              ccAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGt                                                                                    >  2:1859922/1‑149 (MQ=255)
                                                                aaCACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTAt                                                                                  <  1:2034325/149‑1 (MQ=255)
                                                                   acCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaa                                                                               >  1:1516637/1‑149 (MQ=255)
                                                                    ccATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaaa                                                                              <  1:583802/149‑1 (MQ=255)
                                                                    ccATCTCGCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAAAGAAATTAAAAGCATTGTACCGTTAAGGTACAAGTATCTAGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaaa                                                                              <  2:2028576/149‑1 (MQ=255)
                                                                    acATCTATCAGTTTTCTGTCGTTTGACCGACGGATGTCATGGATTGTTTTCATAACGAATTTAAATCCCTTGTACCGTTAAGGTACAAGTATATTTAAGTTTCTTTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaaa                                                                              <  2:1924273/148‑1 (MQ=255)
                                                                           tcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCTGAGGTACATATTGTATGAAAGCGTAtt                                                                       >  1:1360548/1‑149 (MQ=255)
                                                                           tcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTAtt                                                                       >  2:1322558/1‑149 (MQ=255)
                                                                           tcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACAGGAGGTACATATTGTATGAAAGCGTAtt                                                                       >  2:208857/1‑149 (MQ=255)
                                                                            cAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTg                                                                      >  2:1463642/1‑149 (MQ=255)
                                                                               ttttCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTATATGAAAGCGTATTGGGa                                                                   <  1:2142373/149‑1 (MQ=255)
                                                                                   cTGGCGTTAGACCGCCGTATGGCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGActct                                                               <  1:1000351/149‑1 (MQ=255)
                                                                                   cTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGActct                                                               <  2:1808139/149‑1 (MQ=255)
                                                                                   cTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGActct                                                               <  1:636108/149‑1 (MQ=255)
                                                                                    ttgCGTTAGACCGCCGGATGTCATGGAATGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCtt                                                              <  2:230542/147‑1 (MQ=255)
                                                                                    tGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCtt                                                              >  2:1467366/1‑149 (MQ=255)
                                                                                    tGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCtt                                                              >  2:1932142/1‑149 (MQ=255)
                                                                                       cGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTaa                                                           <  2:226579/149‑1 (MQ=255)
                                                                                        gTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAc                                                          <  1:1288986/149‑1 (MQ=255)
                                                                                         ttAGACCGACGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAcc                                                         <  2:613062/149‑1 (MQ=255)
                                                                                           aGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCaa                                                       >  2:1264163/1‑149 (MQ=255)
                                                                                           aGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCaa                                                       <  2:2044073/149‑1 (MQ=255)
                                                                                            taCCGCCGGATGACATGGATTTTTTTCATAACGAAATTAAAACCATTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCaaa                                                      <  1:717326/148‑1 (MQ=255)
                                                                                            gACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCaaa                                                      <  1:1258317/149‑1 (MQ=255)
                                                                                            gACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCaaa                                                      <  2:517889/149‑1 (MQ=255)
                                                                                             cccgccgGATGTCATGGATTGTTCTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAg                                                     <  1:1656193/148‑1 (MQ=255)
                                                                                                 ccgGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGGGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAAca                                                 >  2:880941/1‑149 (MQ=255)
                                                                                                   ggATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTGGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAAcagc                                               >  2:1729392/1‑149 (MQ=255)
                                                                                                     ttGTCATGGATTGTTTTCATAACGAAATTTAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCTATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAAcagcag                                             <  2:1276580/148‑1 (MQ=255)
                                                                                                       gTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGTTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAggg                                           <  2:22196/149‑1 (MQ=255)
                                                                                                       gTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAggg                                           <  1:334896/149‑1 (MQ=255)
                                                                                                        tCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATTTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGc                                          >  2:2119903/1‑149 (MQ=255)
                                                                                                          aTGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACTTATTGTATGAAAGCGTATTGGGACTCTTTACCAAAAGAACAGCAGGGCGa                                        >  2:560253/1‑149 (MQ=255)
                                                                                                           tGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAATGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAg                                       <  1:247616/149‑1 (MQ=255)
                                                                                                           tGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTATCAAAATAACAGCAGGGCGat                                       >  2:206754/1‑148 (MQ=255)
                                                                                                           tGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAg                                       >  1:557270/1‑149 (MQ=255)
                                                                                                           tGGATTGTTTTCAAAAAGAAAATAAAACCCTTGTTCCGTTTATGTACAATTATCTTGTATGTTCAATTCAATTATTTTATATGTACACCGGTGGTAAATATTTTATGAAAGCGTAATGGGACTCTTTAACCAAAGAACAGCAGGGCGAg                                       <  2:554916/149‑1 (MQ=255)
                                                                                                                tGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGc                                  >  1:1037969/1‑149 (MQ=255)
                                                                                                                tGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGc                                  <  2:2086228/149‑1 (MQ=255)
                                                                                                                  ttttCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCg                                >  1:166213/1‑149 (MQ=255)
                                                                                                                   tttCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCgg                               <  1:1547872/149‑1 (MQ=255)
                                                                                                                       aTAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGaaaa                           >  2:364619/1‑149 (MQ=255)
                                                                                                                       aTAAAGAAATTAAACCCCTTGTTCCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCTATCCTGTAATATGTACACCGGATGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGaaaa                           <  2:2031905/149‑1 (MQ=255)
                                                                                                                         aaCGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGt                         >  1:1002679/1‑149 (MQ=255)
                                                                                                                          aCGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGtt                        <  1:482299/149‑1 (MQ=255)
                                                                                                                            taaaTTAAAACCCTTGTACCTTTAAGTTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTgg                      <  2:1086904/148‑1 (MQ=255)
                                                                                                                            caaaTTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTgg                      <  2:1831033/148‑1 (MQ=255)
                                                                                                                             aaaTTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGc                     <  1:758659/149‑1 (MQ=255)
                                                                                                                                 tAAAACCCTTGTACCGTTAAGGTACAGGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCaa                 <  2:92427/149‑1 (MQ=255)
                                                                                                                                   agatacTTGTACCTTAACGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAaca               >  1:1856704/6‑149 (MQ=255)
                                                                                                                                    gatacTTGTACCTTAACGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAacac              >  2:738978/5‑149 (MQ=255)
                                                                                                                                    gatacTTGTACCTTAACGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAacac              >  1:1428912/5‑149 (MQ=255)
                                                                                                                                    aaCCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAGAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAacac              >  1:67598/1‑149 (MQ=255)
                                                                                                                                     aCCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGATAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACAcc             >  1:1134488/1‑149 (MQ=255)
                                                                                                                                      cccTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCt            <  2:707359/149‑1 (MQ=255)
                                                                                                                                             aCCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGCTACt     >  2:1615422/1‑149 (MQ=255)
                                                                                                                                              ccGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTTTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACATGTCTACtt    >  1:619902/1‑149 (MQ=255)
                                                                                                                                               cGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGATCAACACCTGGCTACTTa   >  1:431417/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CCATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGCTACTTAC  >  NC_000913/1202813‑1203105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: