Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009135,1590GA40.7% 143.7 / 64.4 118intergenic (+139/‑75)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (46/24);  new base A (0/48);  total (46/72)
Fisher's exact test for biased strand distribution p-value = 4.01e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGG  >  NC_000913/5012‑5306
                                                                                                                                                    |                                                                                                                                                   
catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAAt                                                                                                                                                  <  2:513942/151‑1 (MQ=255)
catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAAt                                                                                                                                                  <  1:25426/151‑1 (MQ=255)
catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAAt                                                                                                                                                  <  2:922738/151‑1 (MQ=255)
catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAAt                                                                                                                                                  <  1:1443356/151‑1 (MQ=255)
catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAAt                                                                                                                                                  <  2:109352/151‑1 (MQ=255)
   caGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATaac                                                                                                                                               <  2:190092/151‑1 (MQ=255)
    aGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATaaca                                                                                                                                              <  2:34314/151‑1 (MQ=255)
    aGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATaaca                                                                                                                                              <  1:86826/151‑1 (MQ=255)
      tAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATaacaac                                                                                                                                            >  2:654570/1‑151 (MQ=255)
       aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATaacaac                                                                                                                                            <  2:1166514/150‑1 (MQ=255)
        aaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAAccg                                                                                                                                          <  2:736090/151‑1 (MQ=255)
        aaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAAccg                                                                                                                                          >  2:885025/1‑151 (MQ=255)
            cTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGt                                                                                                                                      >  1:537380/1‑151 (MQ=255)
               ttCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTtct                                                                                                                                   >  2:1312675/1‑151 (MQ=255)
                tCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTtctc                                                                                                                                  >  2:948363/1‑151 (MQ=255)
                        tcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGt                                                                                                                           >  1:955440/1‑150 (MQ=255)
                         cAATCAGGCCGGGTTTGTTTTTAGGCACCCCGGTTTTTTTATAAAGAATTTATGGAAAAAAATGAGGGGGAAAAAGGAAAAATTCAAAAAAAAGGCGGTAATTTAGAGTTGAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTa                                                                                                                          <  2:1189359/150‑1 (MQ=255)
                         cAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTa                                                                                                                          >  1:852774/1‑150 (MQ=255)
                          aaTCAGGGTGGTTTTGTTTTTAGGGGCCCCGGTTTTTTTAGAAAGAATTTATGGAGAAAAAGGAGAGGAAAAAAGGAAAATTTCTAAAAAAATGCGGTAATTTAGAATTAAGGATGGC‑GGAGAAAAACAACCGCCGTTCTCATCGAGTAAt                                                                                                                        <  1:570830/151‑1 (MQ=255)
                          aaTCAGGCGGGGTTTTTTTTTATGCGGCCGGTTTTTTTTATAAAGAAATTAGGGAAAAAAATGAAAGGAAAAAAGGAGAAATTCTAAATAAGTGCGGTAACTGAGATTTTAGAATTGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAAt                                                                                                                        <  2:725639/151‑1 (MQ=255)
                          aaTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAAt                                                                                                                        >  2:127169/1‑151 (MQ=255)
                           aTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAAtc                                                                                                                       >  2:608739/1‑151 (MQ=255)
                            ccAGGCGGGGTTTGCTTTTGGAAACCCCGGCTGTTTTAGAAAGAATTAATGAAAAAAAGTGGAAGGAAAAAGGAAAAAATGCCCAATAAAGGCGGAAAGTTAGATATAGGAATGGG‑GGAGATTAACAGCCGCCGTTCTCATCGAGTAAtct                                                                                                                      <  1:705884/150‑1 (MQ=255)
                             cAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAAtctc                                                                                                                     >  2:580017/1‑151 (MQ=255)
                             cAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAAtctc                                                                                                                     >  1:1041100/1‑151 (MQ=255)
                               ggCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCg                                                                                                                   >  1:1287640/1‑151 (MQ=255)
                                   gggTTTGCTTTTATGCAGCCCGGGTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGGAAAAGGAGAAATTCTCAATAAATGCGGGAAATTAGAGATTAGGATTGG‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                                >  1:384712/1‑150 (MQ=255)
                                    gttttGTTTTTATGCAGCCCGGTTTTTTTATAAAAAAATTAGGAAAAAAAATGACAGGAAAAAAGAAAAAATTCTCAATAAATGCGGTAATTTAGAGATTAGGATTGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGatat                                                                                                              <  1:615621/149‑1 (MQ=255)
                                     gtttgtttTTATGCAGCCCGGTTTTTTTAAGAAAAAATTAGGGAAAAAAAGGACAGGAAAAAAGAAAAATTTCTAAAAAAATGCGGTAATTTAGAGATTAGGTGGGC‑GGAGAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc                                                                                                             <  1:955572/151‑1 (MQ=255)
                                        tGCTTTTATGCAGCCCGGTTTTTTTATGAAGAAATTAGGGAAAAAAATGACAGGAAAAAGGAAAAAATTCTAAATAAATGCGGTAACTTAGAGATTAGGATGGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGa                                                                                                           <  2:1383891/150‑1 (MQ=255)
                                        tGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAc                                                                                                          >  2:489432/1‑151 (MQ=255)
                                        tGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAc                                                                                                          >  2:918256/1‑151 (MQ=255)
                                         gCTTTTATGCAGCCCGTTTTTTTTATAAAGAAATTATGGAGAAAAATGACAGGAAAAAAGGAAAAATTCTAAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAc                                                                                                          <  2:86203/150‑1 (MQ=255)
                                           ttttATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAccc                                                                                                        >  2:976627/1‑150 (MQ=255)
                                             ttATGCAGCCCGGTTTTTTTATAAAGAAATTATGGAGAAAAATGACAGGAAAAAAGGAAAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGG‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCAt                                                                                                      <  2:535990/150‑1 (MQ=255)
                                             ttATGCAGCCCGGTTTTTTTATAAAAAAATTAGGGAAAAAAAAGACAGGAAAAAAGGAAAAATTCTCAATAAAGGCGGTAACTTAGAGATTAGGTTTGG‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCAt                                                                                                      <  1:538256/150‑1 (MQ=255)
                                              tATGCAGCCCGGTTTTTTTATGAAGAAATTAGGGAAAAAAATGACGGGGAAAAAGGAAAAATTCTCAATAAATGCGTTAACTTAGAGATTAGGATTGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATa                                                                                                     <  1:270240/150‑1 (MQ=255)
                                              tATGCAGCCCGGTTTTTTTATGAAAAAATTATGGAAAAAAAAGACAGGAAAAAGGAAAAAATTCTAAATAAATGGGGAAATTTAGATATGAGGTTTGC‑GGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATa                                                                                                     <  1:1090478/150‑1 (MQ=255)
                                              tATGCAGCCCGGTTTTTTTAAAAAGAAATTATGGAGAAAAATGACAGGAAAAAAGGAGAAATTCTAAATAAATGCGGTAACTTAGAGATTAGGTTGGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATa                                                                                                     <  2:675370/150‑1 (MQ=255)
                                               atgcccccgggtttttttATAAAAAAATTATGGAAAAAAATGAGAGGAAAAAAGAAGAATTTCAAAATAAATGCGTTATCTAAGAGATTAGATTTGG‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc                                                                                                   <  2:428335/139‑1 (MQ=255)
                                               aTGCAGCCCGGTTTTTTTATAAAAAAATTATGGAAAAAAATGACAGGGAAAAAGGAAAAATTCTAAATAAATGCGGTACCTTAGAGATTAGGATTGC‑GGAAAATAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc                                                                                                   <  2:416095/151‑1 (MQ=255)
                                                      ccGGTTTTTTTATAAAAAAATTAGGAAAAAAAATGACAGGAAAAAAGGAAAAATTCTAAATAAATGCGGTAACTTAGAGATTAGGATGGC‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAAt                                                                                            <  1:397478/151‑1 (MQ=255)
                                                             ttttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa                                                                                      >  2:919287/1‑150 (MQ=255)
                                                             ttttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa                                                                                      >  2:1190181/1‑150 (MQ=255)
                                                              tttaaaaaaaaaTTATGGAAAAAAAGGACAGGAAAAAAGGAAAAATTCTAAATAAATGCGTTAATTTAGAGATTGGGATTGG‑GGAAAAAAAAAGCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaaa                                                                                     <  1:170088/141‑1 (MQ=255)
                                                                tataAAAAAATTAGGGAAAAAAATAACAGGAAAAAAGGAAAATTTCTAAAAAAATGCGGTAACTAAGAGATTAGGATTGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                   <  2:1010791/150‑1 (MQ=255)
                                                                   gaagaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                >  1:966159/1‑150 (MQ=255)
                                                                   gaaaaaATTATGGAAAAAAAGGACAGGAAAAAAGGAGAAATTCTCAATAAAGGCGGTAACTTAGAGATTAGTTTTGC‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                <  2:953765/150‑1 (MQ=255)
                                                                      aaaaTTAGGAAAAAAAATGACAGGAAAAAAGGAAAAATTCTAAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAcc                                                                            <  1:184321/150‑1 (MQ=255)
                                                                        aaTTATGAAAAAAAAGGAAAGGAAAAAAGGAAAAATTCTAAATAAATGCGATAATTTAGAGTTTAGGATTGG‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtg                                                                          <  1:817952/151‑1 (MQ=255)
                                                                          ttATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        >  1:251695/1‑151 (MQ=255)
                                                                           tagggAAAAAAATGACAGGAAAAAAGGAAAAATTCTAAATAAATGCGTTAACTTAGAGATTAGGATTGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        <  2:611450/147‑1 (MQ=255)
                                                                           tagggAAAAAAAGGAAGGGGAAAAAGGAGAAATTCCAAAAAAAGGCGTTAATTTAGAGATGA‑GATCGCAGGAAAACACAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        <  1:1029260/147‑1 (MQ=255)
                                                                           tATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        >  1:311459/1‑150 (MQ=255)
                                                                           tATGGAAAAAAAAGAAAGGAAAAAAGGAAAAATTCAAAAAAAACGCGGTAACTTAGAGATTAGGATTGC‑GGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        <  2:622255/150‑1 (MQ=255)
                                                                           aagggAGAAAAATGACGGGAAAAAAGGAAAAATTCTCAAAAAAGGCGATATTTGAGAGATTAGTATGGC‑GAAGAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        <  2:157764/147‑1 (MQ=255)
                                                                             tGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaa                                                                      >  1:1380747/1‑150 (MQ=255)
                                                                                aaaaaaaGGACGGGAAAAAAGGAAAAATTCACAAAAAGTGCTATAACTAAGAGATTAGGATGGC‑AGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAg                                                                  <  1:968130/149‑1 (MQ=255)
                                                                                 gaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaaa                                                                    >  2:1294079/1‑148 (MQ=255)
                                                                                  aaaaaTGACAGAAAAAAAGGAAAAATCCTAAATAATTGTGTTAACTTAAAAATAAGTATTGC‑GAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGAt                                                                <  1:415321/151‑1 (MQ=255)
                                                                                  aaaaaGGAGGGGAAAAAAGGAAAAATTCAAAAAAATTGGGTTAACTAAGAGATTAGGATGGG‑GGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGAt                                                                <  2:663536/151‑1 (MQ=255)
                                                                                    aaagaacgggaaaaaaGGAGAAATTCTCAAAAAAGGCGGTATTGTAGAGATTAGGATTGG‑GGAGAAAAACATCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGc                                                              <  2:875126/140‑1 (MQ=255)
                                                                                    aaaTGAAAGGAAAAAAGGAAAAATTCTCAATAAATGCGGTAATTAAGAGATTAGGATGGC‑GGAGAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGc                                                              <  2:7058/151‑1 (MQ=255)
                                                                                     aaTGACAGGAAAAAAGGAGAAATTCTAAATAAATGCGGTAACTTAGAGATTAGGATTGG‑GGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCa                                                             <  2:530107/151‑1 (MQ=255)
                                                                                        gagaGGAAAAAGGGAGAAATTCTAAATAAACGCGGTAACTTAAAAATTGGGATTGG‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATc                                                          <  2:508977/148‑1 (MQ=255)
                                                                                        gACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAACAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATc                                                          >  2:274386/1‑151 (MQ=255)
                                                                                         aCAGGAAAAAAGGAAAAATTCTAAAAAAATGCGGTAATTTAGAGATTAGGATTGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCt                                                         <  1:1127194/151‑1 (MQ=255)
                                                                                           aGGAAAAAAGAAAAAATCCCAAAAAAAGGCGGTAATTTAGAGATAAGGATGGC‑GGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTAt                                                       <  1:1312672/151‑1 (MQ=255)
                                                                                              aaaaaaGGAGAAATTCTAAATAAAGGCGTAAACTTAGAGATAGGGATTGG‑GGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                                    <  1:377679/150‑1 (MQ=255)
                                                                                              aaaaaaGGAAAAATTCTAAAAAAATGCGGTAATTTAGAGATTAGGATGGC‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                                    <  2:782726/150‑1 (MQ=255)
                                                                                                 aaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACt                                                 >  1:270081/1‑151 (MQ=255)
                                                                                                 aaaGGAAAAATTCTAAAAAAATGCGATAAGTTAGAGATAAGGATTGC‑GGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACt                                                 <  1:1062982/151‑1 (MQ=255)
                                                                                                  aaGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTc                                                >  1:1011576/1‑151 (MQ=255)
                                                                                                  aaGGAAAAATTCTAAAAAAAGGCGGTAACTTAGAGATTAGGATGGG‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTc                                                <  2:598615/151‑1 (MQ=255)
                                                                                                     gaaaaaTTCTAAAAAAAGGCGGTAATTTAGAGATTAGGATTGG‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCa                                             <  2:537375/148‑1 (MQ=255)
                                                                                                         aaTTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACttt                                         >  1:107538/1‑151 (MQ=255)
                                                                                                               cAATAAATGCGGTAACTTAGATATTAGGATGGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                                     <  2:955445/149‑1 (MQ=255)
                                                                                                                aaaaaaTGCGGTAACTTAGAGATAGGAATGGC‑GGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt                                  <  1:1281399/148‑1 (MQ=255)
                                                                                                                    aaTGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTg                              >  1:371954/1‑151 (MQ=255)
                                                                                                                     aTGCGGTAACTTAGCGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTgg                             >  1:89004/1‑151 (MQ=255)
                                                                                                                      tGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGt                            >  1:1169287/1‑151 (MQ=255)
                                                                                                                       gCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc                           >  2:427015/1‑151 (MQ=255)
                                                                                                                       gCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc                           >  2:1235695/1‑151 (MQ=255)
                                                                                                                        cGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg                          >  2:650512/1‑151 (MQ=255)
                                                                                                                         ggTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGc                         >  2:250871/1‑151 (MQ=255)
                                                                                                                           tAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTc                       >  1:1156538/1‑151 (MQ=255)
                                                                                                                               ttAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAt                   >  2:682130/1‑151 (MQ=255)
                                                                                                                                tAGAGATTAGGATTGC‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCa                    <  2:286113/149‑1 (MQ=255)
                                                                                                                                 agagATTAGGATTGG‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATg                  <  2:575288/150‑1 (MQ=255)
                                                                                                                                  aaaggtAGGTTCGC‑GAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACACATAACGGGCAATGATAAAAGGAGTAGCCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCGTGgc                <  1:192004/146‑1 (MQ=255)
                                                                                                                                    gaTTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgc                >  2:824891/1‑149 (MQ=255)
                                                                                                                                    gaTTAGGATTGC‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcag              <  2:1164763/151‑1 (MQ=255)
                                                                                                                                     ataggaaTTGC‑GGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcagc             <  2:692193/145‑1 (MQ=255)
                                                                                                                                     aTTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcagc             >  1:657917/1‑151 (MQ=255)
                                                                                                                                       tAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAACGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcac           >  2:938714/1‑151 (MQ=255)
                                                                                                                                        gggtttGC‑GAAAAATACAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcaca          <  1:56544/147‑1 (MQ=255)
                                                                                                                                            ttGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCt      >  2:497088/1‑151 (MQ=255)
                                                                                                                                             tGC‑GGAGAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCt      <  2:1424582/150‑1 (MQ=255)
                                                                                                                                                 ggAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg  <  1:252703/151‑1 (MQ=255)
                                                                                                                                                 ggAAAAAAACATCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg  <  1:842886/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGC‑GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGG  >  NC_000913/5012‑5306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑