Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,510,3950CA40.6% 166.5 / 49.8 101S131R (AGC→AGAydcRputative DNA‑binding transcriptional regulator and putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (50/10);  new base A (0/41);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 4.51e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATTAAAC  >  NC_000913/1510245‑1510545
                                                                                                                                                         |                                                                                                                                                       
cACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGc                                                                                                                                                         >  2:1078038/1‑151 (MQ=255)
    cGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCaaaa                                                                                                                                                     <  1:359368/151‑1 (MQ=255)
    cGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCaaa                                                                                                                                                      >  1:309231/1‑150 (MQ=255)
      agaTGAAGCAGTCGATATCAACACTTATATTTTTGATATGCTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAAcc                                                                                                                                                  >  1:810593/1‑151 (MQ=255)
       gaTGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCg                                                                                                                                                 >  1:482779/1‑151 (MQ=255)
       gaTGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCg                                                                                                                                                 <  1:762090/151‑1 (MQ=255)
             gCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCcac                                                                                                                                             >  2:665543/1‑149 (MQ=255)
                  cGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATg                                                                                                                                      >  2:1382064/1‑151 (MQ=255)
                    atatCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATg                                                                                                                                      >  2:1005134/1‑149 (MQ=255)
                      atCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCg                                                                                                                                  >  2:818463/1‑151 (MQ=255)
                      atCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCg                                                                                                                                  >  2:376027/1‑151 (MQ=255)
                            acTTATTTTTTTGATATGTGCCGGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTCCCCGCCCCGCGATTTTCCCCCCCCCAACAACTAACCCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTg                                                                                                                            <  1:1142951/151‑1 (MQ=255)
                            acTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTg                                                                                                                            >  2:583250/1‑151 (MQ=255)
                             tttATTTTTTTAATTTGTTGCAGGCAAGCCGCGATCCGTCGTTCGTTCCTTTTCCCTCGCCCTTCCCCGACCCGCTCTTTTCCCCCCTCAAACAACAAACCCGCTCGCTGGCGAAGGTAAGAAAAACCGCCACAGCGATGAGCGTGAtt                                                                                                                             <  2:605295/148‑1 (MQ=255)
                             cTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTg                                                                                                                            >  2:1068609/1‑150 (MQ=255)
                             cTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTg                                                                                                                            >  1:890789/1‑150 (MQ=255)
                               tattTTTTTGATTTGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTCCCTCGGCCTTCCCCGCCCCGCGATTTTCCCCCCCCAAACAACAAACCCGCCCGCGGGCGCAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTg                                                                                                                            <  2:906520/148‑1 (MQ=255)
                               tataTTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGaa                                                                                                                          >  2:453111/1‑150 (MQ=255)
                               tataTTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGaa                                                                                                                          >  2:271318/1‑150 (MQ=255)
                               tataTTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGaa                                                                                                                          >  2:759047/1‑150 (MQ=255)
                                atatTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGaaa                                                                                                                         >  2:1369834/1‑150 (MQ=255)
                                 tatTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAAc                                                                                                                       >  1:839143/1‑151 (MQ=255)
                                 tatTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAAc                                                                                                                       >  1:471875/1‑151 (MQ=255)
                                  tttttgGATTTTTTGCAGCCCAGCCGCGACCCGTCGGTCTTTCCTTTTCCCCCGCCCTTCCCCGCCCCGCGATTTTCCCCCCCCCAACAACTAACCCGCTCGCTGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAAc                                                                                                                       <  2:594462/149‑1 (MQ=255)
                                  atTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACt                                                                                                                      >  1:1120109/1‑151 (MQ=255)
                                   tttttGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACtt                                                                                                                     >  1:252751/1‑151 (MQ=255)
                                     tttGATATGTTGCAGCCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTCCCCGACCCGCGACTTTTCCCCCCCCAACAACTAACCCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTa                                                                                                                    <  1:229699/150‑1 (MQ=255)
                                           atGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGcc                                                                                                               >  2:1057222/1‑149 (MQ=255)
                                             gtggCAGGCCAGCCGCGACCCTTGGGTCGTCCCTTTTGCCTGGCCCTTCCCCACCCCGCGATTTTCCCCCCCCAAACAACAAACCCGCCCGCGGGCGCGGTTAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAgg                                                                                                            <  2:1076396/147‑1 (MQ=255)
                                             gTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAgg                                                                                                            >  2:101670/1‑150 (MQ=255)
                                               tGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGaa                                                                                                          >  1:735829/1‑150 (MQ=255)
                                                gCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAAc                                                                                                        >  2:870220/1‑151 (MQ=255)
                                                 cAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACg                                                                                                       >  1:251610/1‑151 (MQ=255)
                                                  ggcccattggccacccgccgTTCTTTCCTTTCCCCGCGGCCTTCCCCGCCCCGCGACTTCCCCCCCCCCAACACCAACCCCGCCCGTGGGCGCAGGAAGAAAAAGCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGc                                                                                                      <  1:625687/137‑1 (MQ=255)
                                                  ggcccAGCCGCGATCCGTCGTTGGTCCGTTTTGCCTCGGCCTTTCCCGCCCCGCGTTTTTCCCCCCCCCAACAACAAACCCGCTCGCGGGCGCAGGTAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGc                                                                                                      <  2:908669/148‑1 (MQ=255)
                                                    cccggccgcgtcccGTCGTTCTTTCGTTTTCCCTCGCCTTTCCCCGCCCCGCGTTTTTCCCCCCCCCAACAACTAACCCCCCCGTGGGCGGGGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAg                                                                                                    <  2:1411485/139‑1 (MQ=255)
                                                    cccAGTCGCGCCCCGTCGGTCGTCCCTTTTCCCTCGGCCTTCCCCGCCCCGCGCTTTTCCCCCCCCCAACAACAAACCCGCTCGCTGGCGCAGGAAAGCAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAg                                                                                                    <  2:1068233/150‑1 (MQ=255)
                                                     ccggccgcgACCGGTCGGTCGTCCCTTTTCCCTCGCCTTTCCCCGCCCCGCGATTTTCCCCCCCCAAAAAACTAACCCGCTCGCTGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGa                                                                                                   <  1:378497/146‑1 (MQ=255)
                                                        gccgcgTTCCGCCGGTCGTTCCGTTTCCCCCGCCCTTCCCCCCCCCGCGTTTTTCCCCCCCCCAAAAACAAACCCGCGCGCTGCCGCGGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACt                                                                                                <  1:445522/149‑1 (MQ=255)
                                                          cgcgACCCGTCGGTCGTCCCTTTCCCCCCGCCTTTCCCCCCCCCGCGACTTTCCCCCCCCCAACAACAAACCCGCTCGCGGGCCCAGAAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGc                                                                                              <  1:816700/151‑1 (MQ=255)
                                                           gcgATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCg                                                                                             >  2:407160/1‑151 (MQ=255)
                                                            cgATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGt                                                                                            >  2:318514/1‑151 (MQ=255)
                                                            cgATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGt                                                                                            >  2:1293234/1‑151 (MQ=255)
                                                              aTCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTc                                                                                           >  1:312761/1‑150 (MQ=255)
                                                                ccGTCGTTCTTCCCGTTTCCCCCGCCTTTCCCCGCCCCGCGCTTTTCCCCCCCCAAAAAACAAACCCGCCCGCGGGCGAAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAgg                                                                                        <  2:868118/151‑1 (MQ=255)
                                                                ccGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCGGGCGCAGGGAATCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAgg                                                                                        >  1:262052/1‑151 (MQ=255)
                                                                 cGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGc                                                                                       >  2:776542/1‑151 (MQ=255)
                                                                  gtcgttctTTCCGTTGGCCTCGGCCTTCCCCGCCCCGCGATTTTCCCCCCTCCAACAACAAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCt                                                                                      <  2:1023639/151‑1 (MQ=255)
                                                                  gtcggtcgTCCCGTTTGCCCCGGCCTTCCCCGCCCCGCGATTTTCCCCCCCCAAACAACAAACCCCCCCGCTGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCt                                                                                      <  2:1288601/151‑1 (MQ=255)
                                                                    cggtcgTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTAt                                                                                    >  2:359210/1‑151 (MQ=255)
                                                                        cgTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCt                                                                                >  1:97992/1‑151 (MQ=255)
                                                                         gTTCCTTTTCCCTCGCCTTTCCCCGCCCCGCGATTTTCCCCCCCCCAACAACTAACCCGCCCGCGGGCGCAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtc                                                                               <  2:504820/151‑1 (MQ=255)
                                                                         gTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtc                                                                               >  2:226078/1‑151 (MQ=255)
                                                                          ttcggttgcccccGGCGTTCCCGGCCCCGGTACTTTCCCCCCCCCAAAAACAAACCCGCTCGCGGCCGAAGGAAACAAAAGCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtc                                                                               <  2:1206443/138‑1 (MQ=255)
                                                                          ttCCTTTCGCCCCGGCCTTTCCCGCCCCGCGATTTTCCCCCCACAAAAAATAAACCCCCCCGCGGGCACGGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtc                                                                               <  1:455250/150‑1 (MQ=255)
                                                                          tccctttccccgcGGCTTTCCCCGCCCCGCGATTTTCCCCCCCCCAACAACAAACCCGCCCGCGGGCGCGGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtc                                                                               <  2:545996/138‑1 (MQ=255)
                                                                           tCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGCAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtcgt                                                                             >  2:1069678/1‑151 (MQ=255)
                                                                            ccGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtcgtc                                                                            >  2:458650/1‑151 (MQ=255)
                                                                               tttCCCCCGCCCTTCCCCGCCCCGCGCTTTTTCCCCCCCAAAAAACAAACCCGCTCGCGGGCGCAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGc                                                                          <  2:762427/150‑1 (MQ=255)
                                                                               ttggCCCCGGCTTTCCCGGCCCCGCAATTTTCCCCCCCCCAAAAATAAACCCGCCGGCGGGCGAGGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtcgtcg                                                                           <  1:610606/146‑1 (MQ=255)
                                                                                 cccccaggCTTTCCCCGACCCGCGACTTTTCCCCCCCCAACATCTAACCCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTa                                                                        <  2:480358/144‑1 (MQ=255)
                                                                                  cccTCGCCTTTCCCCGCCCCGCTTTTTTCCCCCCTCAAACAACAAACCCGCTCGCGGGCGCAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATg                                                                      <  2:408798/150‑1 (MQ=255)
                                                                                   ccTCGCCCTTCCCCGCCCCGCGTTTTTCCCCCCCCCAACAACAAACCCGCCCGCGGGCGCGGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGc                                                                     <  2:590660/151‑1 (MQ=255)
                                                                                    cTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGcc                                                                    >  2:810609/1‑151 (MQ=255)
                                                                                      cGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCtt                                                                  >  2:92143/1‑151 (MQ=255)
                                                                                      cGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCtt                                                                  >  2:250668/1‑151 (MQ=255)
                                                                                       ggCCTCCCCCGCCCCGCGCCTTTCCCCCCCCCAACAACAAACCCCCTCGCGGGCGGTGTTAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTa                                                                 <  1:1249750/151‑1 (MQ=255)
                                                                                       gcccTTTCCCGCCCCACGATTTTCCCCCCCCCAACAAACAACCCCGGCGGTGGCGCAGGTAAAAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTa                                                                 <  2:693179/149‑1 (MQ=255)
                                                                                        gCCTTCCCCGCCCGGCGACTTTCCCCCCCCCACCAACAAACCCGCCCGCTGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTAc                                                                <  2:512096/151‑1 (MQ=255)
                                                                                            ttccccGCCCCCCCATTTTCCCCCCCCAAAAAAATAACCGGCACGCTGCCGCAGATAAAAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACa                                                               <  1:975236/146‑1 (MQ=255)
                                                                                               cccTACCCGCCTTTTTCCCCCCTCAAACAAATAAACCGCCTGGGGGGACAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCa                                                          <  1:715196/151‑1 (MQ=255)
                                                                                                 cgccccGCGTTTTTCCCCCCCCCAACAACTAACCCCCCCGCGGGCGCAGGAAAACAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATc                                                        <  2:252744/148‑1 (MQ=255)
                                                                                                  cccccGCGATTTTCCCCCCCCCAACAACAAACCCGCCCGCTGGCGCAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCa                                                       <  2:778650/149‑1 (MQ=255)
                                                                                                          ccTTTCCCCCCCCCAACACCTAACCCCCGCGGTGGCCGAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTtct                                               <  1:1078037/150‑1 (MQ=255)
                                                                                                                 ccccccAAACAACAAACCCGCTCGCTGGCGAAGTAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCtgatg                                        <  2:153291/151‑1 (MQ=255)
                                                                                                                 ccccTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCtgatg                                        >  2:1208572/1‑151 (MQ=255)
                                                                                                                   ccTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCtgatga                                       >  2:708465/1‑150 (MQ=255)
                                                                                                                     cccaaacaacAAACCCGCCCGCGGGCGCAGGAAACAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGaaa                                     <  1:708471/147‑1 (MQ=255)
                                                                                                                       caaacaacAAACCCGCGCGCGGGCGCAGAAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAAtt                                   <  1:1216095/149‑1 (MQ=255)
                                                                                                                         aacaacAAACCCGCTCGCGGGCGCAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGt                                 <  1:1005132/151‑1 (MQ=255)
                                                                                                                          acaacaAACCCGCTCGCTGGCGAAGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTc                                <  2:1448327/151‑1 (MQ=255)
                                                                                                                             acTAACCCGCGCGCGGGCGCAGGAAAACAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCAtt                             <  2:121605/151‑1 (MQ=255)
                                                                                                                                aaaCCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACt                          >  1:226809/1‑151 (MQ=255)
                                                                                                                                  acccgcccgcGGGCGCAGATAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTg                         <  1:367546/149‑1 (MQ=255)
                                                                                                                                    cccctggttgccgcAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGcc                       <  2:890791/140‑1 (MQ=255)
                                                                                                                                      ccccgctGGCGCAGTTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCgg                     <  2:1415631/148‑1 (MQ=255)
                                                                                                                                       cccgggggCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCggg                    <  1:271324/145‑1 (MQ=255)
                                                                                                                                          gctGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCg                 >  2:1415200/1‑151 (MQ=255)
                                                                                                                                           ctGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGt                >  1:130694/1‑151 (MQ=255)
                                                                                                                                            tGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGtt               >  2:1375807/1‑151 (MQ=255)
                                                                                                                                             ggCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGtt               >  2:1389644/1‑150 (MQ=255)
                                                                                                                                             ggCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGtt               >  2:300361/1‑150 (MQ=255)
                                                                                                                                              gggaagaaaaGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTag             <  2:1387208/143‑1 (MQ=255)
                                                                                                                                               cgcAGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTaga            <  1:1083314/151‑1 (MQ=255)
                                                                                                                                                  gggaaaGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGc         <  1:503665/147‑1 (MQ=255)
                                                                                                                                                  aGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGc         >  1:670931/1‑151 (MQ=255)
                                                                                                                                                      aaGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATTa     <  2:769128/151‑1 (MQ=255)
                                                                                                                                                        gCAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTTGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATTaaa   <  2:839142/151‑1 (MQ=255)
                                                                                                                                                        gCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATTaa    >  1:12967/1‑150 (MQ=255)
                                                                                                                                                        acAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATTaaa   <  2:889845/150‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                       
CACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATTAAAC  >  NC_000913/1510245‑1510545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: