Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA41.8% 138.7 / 56.0 103intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (42/18);  new base A (0/43);  total (42/61)
Fisher's exact test for biased strand distribution p-value = 6.90e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29347‑29642
                                                                                                                                                       |                                                                                                                                                  
ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    >  1:767278/1‑150 (MQ=255)
 tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                   <  1:425248/150‑1 (MQ=255)
  aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                  <  2:1282087/150‑1 (MQ=255)
  aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                  >  2:761292/1‑150 (MQ=255)
     gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                               >  1:1345318/1‑150 (MQ=255)
      tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                             >  2:672064/1‑151 (MQ=255)
       aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                             <  1:1018899/150‑1 (MQ=255)
         aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                          <  2:454241/151‑1 (MQ=255)
           tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                         <  2:1109900/150‑1 (MQ=255)
           tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                                        >  1:835769/1‑151 (MQ=255)
           tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                                        >  2:980257/1‑151 (MQ=255)
            ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       >  1:482663/1‑151 (MQ=255)
            ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                                        <  1:1122538/150‑1 (MQ=255)
             tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       >  2:123824/1‑150 (MQ=255)
             tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       <  2:892663/150‑1 (MQ=255)
              gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                      <  2:1348819/150‑1 (MQ=255)
              gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                      <  1:870689/150‑1 (MQ=255)
                   tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                                >  1:490191/1‑151 (MQ=255)
                   tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                                >  1:408011/1‑151 (MQ=255)
                    ttggcccctttttttCTGCCTTTTTTTATTTCATGCAATCTTTTTACTGCGGATGCTTTTGCAAGAACGGATGAGATGTTCTTTTTGTCGTTAAAGG‑CC‑TTAAAACCATCCATGCGCCAAAAAAAAAAATAAAAAATCCCGCCATTAAGt                                                                                                                                <  1:42338/141‑1 (MQ=255)
                    ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                               >  1:922402/1‑151 (MQ=255)
                      ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                              >  1:1032818/1‑150 (MQ=255)
                      ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                              >  2:301097/1‑150 (MQ=255)
                         ccACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtt                                                                                                                          >  2:1173182/1‑151 (MQ=255)
                            tttttttCTGCTCTTTTTTTTTTCATGCAATCTTTTTGCTGCGAAAGCTTTTTCAAGAACAGTTTAGATAATTTTTTTGTCGCTTAATG‑CC‑TGAAAAACATGCATGACCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                         <  1:611187/148‑1 (MQ=255)
                               ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                    >  1:1344111/1‑151 (MQ=255)
                                tttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                                   >  1:1445224/1‑151 (MQ=255)
                                     gctttttttttttTAATGAAATCTTTTTGCTGCGCAGGCTTTTCCAAAAACGGGTAAGATTATTTTTTTGTCGTTTAAGG‑CG‑TTTAAAACATGCATAAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                               <  2:474755/145‑1 (MQ=255)
                                       ttttttttATTTCATGCAATCTTCTTGCTGCCCAAGCTTTTCCCAAAACGGTTTAGATGTTTTTTTTGCCGCTAAATG‑CC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                             <  2:455461/147‑1 (MQ=255)
                                          tttttATTTCATGCAATCTTCTGGCTGCGCAAGCGTTTCCCAAAACAGGTTAGATAATTTTTTTGTCGCTTAATG‑CC‑TTAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                          <  1:648541/150‑1 (MQ=255)
                                           ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  2:301358/1‑150 (MQ=255)
                                            tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  2:695504/1‑149 (MQ=255)
                                             ttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  2:246950/1‑148 (MQ=255)
                                              tATTTCATCCATTCTTCTTGCGGCGCAAGCTTTTCCAAGAACGGGTTAGATTATTTTTTTGTCGCTAAATG‑CC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                      <  2:34851/150‑1 (MQ=255)
                                               ttttCATGCAATCTTCTTGCTGCGCAAGCTTTTTCCAAAACAGGTTAGATGTTTTTTTTGTCGTTTAATG‑CC‑TTAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGaa                                                                                                     <  2:194792/149‑1 (MQ=255)
                                               aTTTCATGCATTCTTCTTCCTGCGCAGGCTTTTTCAAGAACAGGTTAGATGATTTTTTTGTCGCTTAATG‑CC‑TGAAAAACATGCATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGaa                                                                                                     <  1:1390968/150‑1 (MQ=255)
                                                 tccATGAAATCTTTTTGCTGCGCAGGCGTTTCCAAAAACAGGTTAAATGATCTTTTTCTCTCTAAATG‑CT‑TGAAAAACTTTCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                                   <  2:1065489/148‑1 (MQ=255)
                                                   cATGCAATCTTCTTGCTGCGCAAGCTTTTTCCAGAACAGTTTAGTTTATCTTTTTGTCGTTTAATG‑CC‑TTTAAAACATGCATGGGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                <  2:1351556/151‑1 (MQ=255)
                                                    aTGAAATCTTCTTGCTGCGCAAGCTTTTCCAAAAACGGTTTAGATGATTTTTTTGTCGCTAAATG‑CT‑TGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgc                                                                                               <  2:1437550/151‑1 (MQ=255)
                                                      gCAATCTTCTTGCTGCGCAAGCTTTTCCAAAAACAGTTTAGATGATTTTTTTGTCGTTAAATG‑CC‑TGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                              <  1:1124624/150‑1 (MQ=255)
                                                        aaTTTTTTTGCTGCCAAAGTTTTTTCAAGAACAGTTTGTAGATTTTTTTTGTCG‑TTAATGCCC‑TGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                           <  2:265035/151‑1 (MQ=255)
                                                        aaTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                           >  2:1283556/1‑151 (MQ=255)
                                                             tcttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                       >  2:1102017/1‑150 (MQ=255)
                                                                tgttgggaatgCGTTTTCAAAAACAGTTTAGATATTTTTTTTTTCG‑TTAATT‑CCTTATAAAACATGCATGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                    <  1:223039/140‑1 (MQ=255)
                                                                tgctgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                    >  2:1031720/1‑150 (MQ=255)
                                                                tcctgcGCAGGCTTTTCCAAAAACAGTTAAGATGATTTTTTTGTCGCTTAATG‑CC‑TTAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                    <  2:871111/148‑1 (MQ=255)
                                                                 gctgcGCAAGCGTTTTCCAGAACGGGTTAGATAATTTTTTTGTCGCTAAATG‑CC‑TAAAAAACATGCAGGGGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                   <  2:1393945/150‑1 (MQ=255)
                                                                 cctgcGCATCCTTTTTCCAGAAAGGTTTAGATAATTTTTTTGTATTTAAAGG‑CT‑TGAAAAACACTCATGACCCACAAAAAAAAATAAAAACACCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                  <  1:1185470/150‑1 (MQ=255)
                                                                    gcgcAAGCTTTTCCAAAAACAGGTTAAATAATTTTTTTGTCGCTAAATC‑CC‑TAAAAAACATCCATGAGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                               <  1:6820/151‑1 (MQ=255)
                                                                    gcgcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                               >  2:1361723/1‑151 (MQ=255)
                                                                     cgcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                              >  2:1256656/1‑151 (MQ=255)
                                                                       cAAGCTTTTTCAAAAAAAGTTAAGATGATTTTTTTTTCGTTTAATG‑CC‑TTAAAAACATGAATGAGCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                            <  1:1161953/151‑1 (MQ=255)
                                                                        aaGCTTTTTCCAGAACAGGTTAGATGATTTTTTTGTCGTTTAATG‑CT‑TGAAAAACATGCATGAGCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                           <  2:377952/151‑1 (MQ=255)
                                                                          gttttttCCAAAAAAGGTTAGATGATTTTTTTGCCGTTTATTT‑CC‑TGAAAACAAGGCATGACCCACAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                         <  1:171229/148‑1 (MQ=255)
                                                                            gTTTTCCAGAACAGGTTAGATGATCTTTTTGTAGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                        >  1:868731/1‑150 (MQ=255)
                                                                               ttcaaaaaCGGGTTAGAGGATTTTTTTGTCGTTTAATG‑CC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                     <  2:210350/144‑1 (MQ=255)
                                                                                 caaGAACAGTTAAAATGTGCTTTTTGTCGCTTATCT‑CT‑TTAAAAACGGCCATGCCCCAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGATTGTGAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAg                                                                   <  2:353585/148‑1 (MQ=255)
                                                                                    aaaCAGTTTAGATGATTTTTTTGTCGTTTAATG‑CC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                               <  2:1075281/150‑1 (MQ=255)
                                                                                      aCAGGTTAGATGATTTTTTTGTCGCTTAATC‑CC‑TGTAAACCATGAATGACCCACAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                             <  1:530063/151‑1 (MQ=255)
                                                                                            tAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                       >  1:1003665/1‑151 (MQ=255)
                                                                                             aGATGATTTTTTTGTCGTTTAATG‑CC‑TAAAAAACATGCATGACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                                      <  2:547898/151‑1 (MQ=255)
                                                                                              aattttttttttGTCGTTTAATG‑CT‑TGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                     <  1:797141/144‑1 (MQ=255)
                                                                                                    ttttttGTCGCTTAATG‑CT‑TGTAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  1:63721/149‑1 (MQ=255)
                                                                                                    ttttttCTCTCTTAATG‑CC‑TGTAAAACATGCATAACCCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  1:288745/149‑1 (MQ=255)
                                                                                                     ttttttTCGCTAAATG‑CC‑TGAAAAACGTCGTGGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                               <  2:753376/150‑1 (MQ=255)
                                                                                                      ttttGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                              <  2:918996/150‑1 (MQ=255)
                                                                                                         ggTCGCTTAATG‑CC‑TGAAAAACATGAAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                           <  2:94829/149‑1 (MQ=255)
                                                                                                          gTCTCTTAATG‑CC‑TTAAAAACATGCATGACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         <  1:1190360/151‑1 (MQ=255)
                                                                                                          gTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         >  2:225876/1‑151 (MQ=255)
                                                                                                             gCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       >  1:152528/1‑150 (MQ=255)
                                                                                                               ttAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    >  1:764137/1‑151 (MQ=255)
                                                                                                                tAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   >  2:1420563/1‑151 (MQ=255)
                                                                                                                 attG‑CC‑TGTAAACCATGAATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  <  2:835768/149‑1 (MQ=255)
                                                                                                                 aaTG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   >  2:141971/1‑150 (MQ=255)
                                                                                                                 aaTG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   >  2:571292/1‑150 (MQ=255)
                                                                                                                  aTG‑CC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                 <  2:408003/151‑1 (MQ=255)
                                                                                                                  aTG‑CC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                 <  1:1337943/151‑1 (MQ=255)
                                                                                                                  aTG‑CC‑TGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                 <  1:962503/151‑1 (MQ=255)
                                                                                                                    g‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                >  2:785406/1‑150 (MQ=255)
                                                                                                                    g‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                >  2:172982/1‑150 (MQ=255)
                                                                                                                       cctaaaaaaCATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                              <  1:66758/146‑1 (MQ=255)
                                                                                                                           tAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                           >  2:680576/1‑150 (MQ=255)
                                                                                                                             aaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        >  2:848116/1‑151 (MQ=255)
                                                                                                                             aaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        >  2:1271315/1‑151 (MQ=255)
                                                                                                                              aaCATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                       <  2:826547/151‑1 (MQ=255)
                                                                                                                               aCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                       >  1:1339896/1‑150 (MQ=255)
                                                                                                                               aCATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                      <  1:682581/151‑1 (MQ=255)
                                                                                                                                   gcatgAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  <  2:241112/151‑1 (MQ=255)
                                                                                                                                    catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  >  2:912119/1‑150 (MQ=255)
                                                                                                                                     atgACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                <  1:695509/151‑1 (MQ=255)
                                                                                                                                     aggAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                <  2:716295/149‑1 (MQ=255)
                                                                                                                                        aGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga             <  1:1115133/151‑1 (MQ=255)
                                                                                                                                             aaaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt        <  2:1344113/150‑1 (MQ=255)
                                                                                                                                              aaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       <  2:767271/151‑1 (MQ=255)
                                                                                                                                              aaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       <  2:1267499/151‑1 (MQ=255)
                                                                                                                                                 ataataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct    >  1:222359/1‑151 (MQ=255)
                                                                                                                                                   aatnTAAAANNNNCNGNCANNCAGTTGACTTTTAGCGCCCATANNTAGAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAGTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  1:1077687/1‑151 (MQ=255)
                                                                                                                                                   aataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  2:185325/1‑151 (MQ=255)
                                                                                                                                                   aataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg   <  2:868733/150‑1 (MQ=255)
                                                                                                                                                   aataAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  <  2:822598/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
TTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29347‑29642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑