Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	41.8%	178.1 / 22.5	98	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (48/9);  new base C (0/41);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 3.71e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443127‑3443423                                                                                                                                                         |                                                                                                                                                   gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                                                                    <  2:126912/151‑1 (MQ=255)  cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                   >  1:1357310/1‑151 (MQ=255)    gACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccc                                                                                                                                                 <  2:43316/151‑1 (MQ=255)     aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                                                                >  2:314478/1‑151 (MQ=255)       gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                                                              >  1:812393/1‑151 (MQ=255)       gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                                                              >  2:221108/1‑151 (MQ=255)        cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGc                                                                                                                                             <  1:592013/151‑1 (MQ=255)           agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                          >  1:727173/1‑151 (MQ=255)             agTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCatat                                                                                                                                        >  2:910288/1‑151 (MQ=255)               tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTc                                                                                                                                      >  1:459537/1‑151 (MQ=255)                 acacAAAAAGCGGTTCCCCAGGCTAAAAAAACAGCGAAATTGTTGTCAGCCAGTTCAACCCAGTCCCCCCCCCA‑AA‑CCATGCCGCGAGGGTCCCGGGAAAAAAAATAATACTGGAAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCAc                                                                                                                                    <  1:931010/151‑1 (MQ=255)                  cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACt                                                                                                                                   >  2:950604/1‑151 (MQ=255)                    aaTAAAGCGTTACCCCGGCCTAAAAATACAGGAAAATTGTTGAAACCCATTTCCAACCAGCACCCCCCCCA‑AA‑CCAAGACGCTTTGGTCGCGGGGAACAAAAAAATTTTGGAGGAGAAGATCCCGGGGATTACCCCCGCCATATTCACttt                                                                                                                                 <  1:471012/150‑1 (MQ=255)                     aaaagggggtgccccAGGCTGAAAATACAGCAAATTTGTTGCCAGCCAGTCCCACCCAGCCCCCCCCCCA‑AC‑CCATGACGCGAGGGTCGCCGGGAACAAAATAATACTGGAAGCGAAGTTTGCGGGAATTCCCCCCGCCATATTCACTTTc                                                                                                                                <  1:833720/139‑1 (MQ=255)                       aaaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                >  1:323774/1‑149 (MQ=255)                        aaGCGGTTGCCCGGGCTGCAAATACAGCAAAATTGTTGCCAGCCAGTTCAACCCAGTCCCGCCCCCG‑AA‑CCATGACGCGAGGGTCGCCGGAAACAAAATAATCCTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGc                                                                                                                             <  2:1389582/151‑1 (MQ=255)                        aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGc                                                                                                                             >  1:137710/1‑151 (MQ=255)                           gggTTGCCCAGGGTGAAAATACAGGAAAATTGTTGTCAGCAAGTTCCAACCAGTCCCCCCCCCA‑AA‑CCAGGACGGGATGGCCGCGGGAAAAAAAATAATACTGGAGGAGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGt                                                                                                                          <  1:1350102/150‑1 (MQ=255)                           cGGTTCCCCAGGCTGCAAATACGGCGAAATTGTTGTCAGCCATTTCCACCCAGTCCCCCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAAAAAAATAATCCTGGAAGCGAAGATTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGt                                                                                                                          <  2:1070700/151‑1 (MQ=255)                               tgccagggctaaaaaTACAGCAAATTTGTTGTCAGCCAGTTCAAACCAGTCCCGCCCCCG‑AA‑CCATGCCGCGATGGTCGCCGGGAAAAAAATAATACTGGAGGCGAAGATTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                       <  1:1169505/138‑1 (MQ=255)                               tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                      >  2:355695/1‑151 (MQ=255)                                gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                     >  2:1025390/1‑151 (MQ=255)                                gCCCAGGCTAAAAAAACAGCAAAATTGTTGCCAGCCAGTCCCAACCAGTCCCGCCCCCA‑AA‑CAATGACGCGATGGTGGCGGGAAACAGAAAAATTCGGGAAGCGAGGATGGCGGGAATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                      <  2:1129667/150‑1 (MQ=255)                                gCCAAGGCTGAAAATACAGCAAATTTGTTGTCAGCCAGTCCCAACCAGTCCCCCCCCCG‑AA‑CCATGACGCGATGGTCGCGGGGAACAAAATAATACTGGAAGCGAAGATTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                      <  2:362780/150‑1 (MQ=255)                                    aGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                                 >  1:81048/1‑151 (MQ=255)                                     ggCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                >  1:1305617/1‑151 (MQ=255)                                     ggCTGCAAATACAGAAAATTTGTTGTCAGCCATTTCCACCCAGTACCGCCCCCG‑AC‑CCATGCCGCGATGGTCGCCGGGAACAGAATAATCCTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                <  2:584667/151‑1 (MQ=255)                                      gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                               >  1:1310090/1‑151 (MQ=255)                                         gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                            >  1:560000/1‑151 (MQ=255)                                         gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                            >  2:963450/1‑151 (MQ=255)                                            aaTACAGCGAATTTGTTGTCAGCCATTTCCACCCATTCCCCCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                         <  1:982256/151‑1 (MQ=255)                                              tACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                         >  2:103947/1‑149 (MQ=255)                                              cacagtaaatttgttgTCGGCCA‑TCCCAAACACGCCCCCCCCCGCAA‑CAATGACGAGCTGGTCGCTGGGAACAGAAAAAAACCGGAAGAAAAGATTGCGGGGATCCCCGCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcag                                                                                                       <  2:453599/141‑1 (MQ=255)                                               aCAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                      >  2:444977/1‑151 (MQ=255)                                                    gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                    >  2:710802/1‑148 (MQ=255)                                                     aatttgttgTCAGCCAGTCCAAACCAGTCCCGCCCCCA‑AA‑CCATGACGGGGTGGTCCCGGGAAACAAAAAAATCTTGGAAGAGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                <  2:590793/148‑1 (MQ=255)                                                      aatggttttacgCCAGTGCCACCCATCCCCCCCCCCG‑AA‑CCGTGGCGGGGTGGGCGCGGGAAACAAAAAAATCCGGGGAGCGAATTTGGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                               <  1:480975/140‑1 (MQ=255)                                                       aTTGTTGTCGGCCATTTCCAACCAGTCCCGCCCCCG‑AA‑CCATGGCGCGAGGGTCGCCGGAAACAAAATAATCCGGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                              <  2:449777/151‑1 (MQ=255)                                                         tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                             >  1:1133998/1‑150 (MQ=255)                                                         tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                             >  1:671127/1‑150 (MQ=255)                                                         tgttcccacccAGTTCCAACCACT‑CCCCCCCCG‑AACCCAGGACGCGAGGGTCGCCGGCAACAAAAAATAACTGGAAGCGAAGATTGCCGGGTTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                             <  1:1121301/141‑1 (MQ=255)                                                          gttgtCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                           >  2:379914/1‑151 (MQ=255)                                                           ttgcCAGCCGGTCCCACCAATTCCCGCCCCCG‑AC‑CCAGGACGCGATGGTCGCGGGGACCAAAAAAATACTGAAGGCGAAGATTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                           <  2:33207/150‑1 (MQ=255)                                                                aGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                     >  1:769038/1‑151 (MQ=255)                                                                   cattcccacccacTCCCGCCCCCG‑AA‑CCATGACGCGATGGCCGCGGGGAAAAGAATAATACGGGAAGCGAAAATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                  <  2:1167275/146‑1 (MQ=255)                                                                    atttCCAACCAGTCCCCCCCCCG‑AA‑CCATGACGCGAGGGTCGCCGGGAACAAAATAATACTGGAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                 <  2:1078361/149‑1 (MQ=255)                                                                     gTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                 >  1:722199/1‑150 (MQ=255)                                                                        ccAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                             >  1:367131/1‑151 (MQ=255)                                                                         cAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                            >  1:929248/1‑151 (MQ=255)                                                                          acccAGTCCCCCCCCCG‑AC‑CCACGCCGGGATGGCCCCGGGCAACAAAAAAATACGGGAAGGAAAGATCGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                           <  2:531837/149‑1 (MQ=255)                                                                          aaCCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                           >  1:1434330/1‑151 (MQ=255)                                                                              aGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                       >  1:1166116/1‑151 (MQ=255)                                                                                tACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                      >  2:1222281/1‑150 (MQ=255)                                                                                   cccccccA‑AC‑CCATGACGGGATGGCCCCGGGAAAAAAAAAAATACGGGAAGCAAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                  <  1:918737/149‑1 (MQ=255)                                                                                   cGCCCCCG‑AC‑CCATGACGCGAGGGTCGCCGGGAACAAAATAATACGGGAAGCGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                  <  2:1357307/151‑1 (MQ=255)                                                                                    ccccccG‑AC‑CCATGCCGGGATGGTCGCCGGGAACAGAATAATACGGGAAGCGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                 <  2:1055620/150‑1 (MQ=255)                                                                                           caacccATGCCGGGATGGCCGCCGGAAACAAAAAAATCCGGGAAGCGAAGTTTCCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                            <  2:1397484/147‑1 (MQ=255)                                                                                                aaggACGGGAGGGGCGGCGGAAAAAAAATAATACTGGAAGCAAAGATTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                       <  1:186569/148‑1 (MQ=255)                                                                                                 aTGCCGCGATGGTCGCGGGAAACAAAATAATAGTGGAAGCGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                      <  1:272445/151‑1 (MQ=255)                                                                                                    aCGGGAGGGTCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                   <  1:379902/151‑1 (MQ=255)                                                                                                      gggATGGCCGCGGGAAACAAAATAATAGGGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                 <  2:1382720/149‑1 (MQ=255)                                                                                                         aTGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                              >  2:1034204/1‑151 (MQ=255)                                                                                                          tGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                             >  1:722902/1‑151 (MQ=255)                                                                                                          gggccgcgGGAAAAAGAATAATACGGGAAGGGAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                             <  1:1136142/147‑1 (MQ=255)                                                                                                               gCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                        >  2:348130/1‑151 (MQ=255)                                                                                                                     aaCAGAATAATACTGGAAGGGAAGATTGCCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                  <  2:582299/151‑1 (MQ=255)                                                                                                                     aaCAAAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                  <  2:1042865/151‑1 (MQ=255)                                                                                                                       cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  1:115635/1‑151 (MQ=255)                                                                                                                       aaaaaaaataCGGGAAGGAAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                <  2:72495/145‑1 (MQ=255)                                                                                                                        aGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  1:1004037/1‑150 (MQ=255)                                                                                                                          aataatACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                             <  2:1294541/151‑1 (MQ=255)                                                                                                                          aataatACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                              >  1:508118/1‑150 (MQ=255)                                                                                                                             aataCTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                          <  2:1356668/151‑1 (MQ=255)                                                                                                                             aataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                           >  1:768569/1‑150 (MQ=255)                                                                                                                              ataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                          >  2:236984/1‑150 (MQ=255)                                                                                                                              ataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                          >  1:802803/1‑150 (MQ=255)                                                                                                                              ataCGGGAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                         <  2:1223764/151‑1 (MQ=255)                                                                                                                                gctgaaggCGAAGTGTGCCGGAATTCCCGCCGCCATATTCGCTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTGCCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                         <  2:137703/142‑1 (MQ=255)                                                                                                                                ccggaaaGCGAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                        <  1:992644/145‑1 (MQ=255)                                                                                                                                acgggaggaaaaGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                        <  1:451634/140‑1 (MQ=255)                                                                                                                                 cgggAAGCGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                      <  2:1226132/149‑1 (MQ=255)                                                                                                                                  gggAAGAAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                     <  1:1083454/150‑1 (MQ=255)                                                                                                                                   ggAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                    <  2:323768/151‑1 (MQ=255)                                                                                                                                    aaggCGAAGTTTCCCGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                   <  1:613951/148‑1 (MQ=255)                                                                                                                                         gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt              >  1:540707/1‑151 (MQ=255)                                                                                                                                         gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt              >  2:1042941/1‑151 (MQ=255)                                                                                                                                         gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt              >  1:1288626/1‑151 (MQ=255)                                                                                                                                          aattttGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg             <  1:1106366/147‑1 (MQ=255)                                                                                                                                          aaGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg             >  2:594930/1‑151 (MQ=255)                                                                                                                                           aGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc            >  1:630064/1‑151 (MQ=255)                                                                                                                                              ttGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa          >  2:489651/1‑150 (MQ=255)                                                                                                                                               ggcggggTTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat        <  1:152306/147‑1 (MQ=255)                                                                                                                                               cgCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat        <  1:1417007/150‑1 (MQ=255)                                                                                                                                                gcggggATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat        <  2:809626/147‑1 (MQ=255)                                                                                                                                                gCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat        >  2:776541/1‑150 (MQ=255)                                                                                                                                                 ccGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac      <  2:1305620/151‑1 (MQ=255)                                                                                                                                                   gggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta    <  2:1258131/151‑1 (MQ=255)                                                                                                                                                      aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat  >  1:1365294/1‑150 (MQ=255)                                                                                                                                                      aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat  >  1:633143/1‑150 (MQ=255)                                                                                                                                                         |                                                                                                                                                   GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCG‑AA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443127‑3443423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑