Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,818,103 A→T V133E (GTA→GAA)  chbF ← phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,818,1030AT98.7% 321.1 / ‑6.7 78V133E (GTA→GAA) chbFphospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (45/32);  total (46/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATAAACATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTA  >  NC_000913/1817955‑1818249
                                                                                                                                                    |                                                                                                                                                  
aaTAAACATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCg                                                                                                                                                  >  1:812120/1‑151 (MQ=255)
 aaaaaCATCTTCATGCGGATCGAAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGa                                                                                                                                                 <  2:870057/149‑1 (MQ=255)
 aTAAACATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTACGACATCTTTTTCg                                                                                                                                                  >  1:1341873/1‑150 (MQ=255)
  tAAACATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGAt                                                                                                                                                >  2:12740/1‑151 (MQ=255)
  aaaaCATCTTCAGGCCGATCGAAATTTTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGAt                                                                                                                                                <  1:909457/150‑1 (MQ=255)
   aaaCATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATg                                                                                                                                               >  2:5071/1‑151 (MQ=255)
     aCATCTTCATCCCGATGGGAATATTCCCCACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTc                                                                                                                                             <  1:1103910/151‑1 (MQ=255)
      cATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTc                                                                                                                                             >  2:89784/1‑150 (MQ=255)
           tCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAAt                                                                                                                                       >  2:1351634/1‑151 (MQ=255)
           tCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAATTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAAt                                                                                                                                       >  2:884036/1‑151 (MQ=255)
           actcgcggaacgaaaTATTACGCACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCaaaaa                                                                                                                                        <  1:1286741/137‑1 (MQ=255)
            cATGCCGATGGAAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATc                                                                                                                                      <  1:127331/151‑1 (MQ=255)
            cATGCCGATCGGAATATTACCCACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAAt                                                                                                                                       <  1:1300344/150‑1 (MQ=255)
               cccGATCGAAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCAcc                                                                                                                                   <  2:230890/150‑1 (MQ=255)
                  gatggaaaTTTTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGa                                                                                                                                <  1:58869/145‑1 (MQ=255)
                     cgaaaTATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAAt                                                                                                                              <  1:311012/147‑1 (MQ=255)
                        aaTATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGt                                                                                                                           >  2:194142/1‑150 (MQ=255)
                        aaTATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTa                                                                                                                          <  1:429366/151‑1 (MQ=255)
                         atatTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTAc                                                                                                                         <  2:461018/151‑1 (MQ=255)
                           atTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGc                                                                                                                       >  2:15307/1‑151 (MQ=255)
                           atTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGc                                                                                                                       >  2:893362/1‑151 (MQ=255)
                            ttACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGc                                                                                                                       >  1:834577/1‑150 (MQ=255)
                                ccacGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGAcc                                                                                                                  <  1:23809/150‑1 (MQ=255)
                                      cGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaa                                                                                                             >  1:793671/1‑150 (MQ=255)
                                         tAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaaaca                                                                                                          >  2:424587/1‑150 (MQ=255)
                                              cGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAAccgc                                                                                                    <  2:299259/151‑1 (MQ=255)
                                               gTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAAccgcc                                                                                                   >  1:383858/1‑151 (MQ=255)
                                               gTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAAccgcc                                                                                                   >  2:1124662/1‑151 (MQ=255)
                                               gTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAAccgcc                                                                                                   >  2:111853/1‑151 (MQ=255)
                                                    aaTCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCGCCGGAATGGTACGCAGACCTTTAAACAAACCGCCCgcgc                                                                                              >  1:751099/1‑151 (MQ=255)
                                                    aaTCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCgcgc                                                                                              <  2:482979/151‑1 (MQ=255)
                                                       ccGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCg                                                                                            <  1:42703/150‑1 (MQ=255)
                                                        cGGTAGGACGATAAACGGTTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGtt                                                                                          <  1:746216/151‑1 (MQ=255)
                                                         ggTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTg                                                                                         >  1:1347881/1‑151 (MQ=255)
                                                          gTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTgg                                                                                        >  1:382227/1‑151 (MQ=255)
                                                           tATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGt                                                                                       >  2:977469/1‑151 (MQ=255)
                                                             tGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGttt                                                                                     >  1:340660/1‑151 (MQ=255)
                                                              gACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTc                                                                                    >  2:1110211/1‑151 (MQ=255)
                                                                cGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCt                                                                                  <  2:1280087/151‑1 (MQ=255)
                                                                 gATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTg                                                                                 <  2:47755/151‑1 (MQ=255)
                                                                 gATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTg                                                                                 >  1:114615/1‑151 (MQ=255)
                                                                 gATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTg                                                                                 >  2:124912/1‑151 (MQ=255)
                                                                  aTAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGa                                                                                <  2:862627/151‑1 (MQ=255)
                                                                  aTAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGa                                                                                <  1:92857/151‑1 (MQ=255)
                                                                      aCGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCaa                                                                            <  1:1114477/151‑1 (MQ=255)
                                                                       cGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCa                                                                             >  1:1367861/1‑149 (MQ=255)
                                                                        gtcTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGa                                                                          <  1:898228/149‑1 (MQ=255)
                                                                        ggCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCa                                                                             >  1:766178/1‑148 (MQ=255)
                                                                            tCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATa                                                                        <  1:1411780/149‑1 (MQ=255)
                                                                             cAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAAcc                                                                     >  1:991627/1‑151 (MQ=255)
                                                                                   ccATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACt                                                               >  2:936817/1‑151 (MQ=255)
                                                                                    cATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACtt                                                              >  1:99142/1‑151 (MQ=255)
                                                                                     aTTCCCGCCGGGTTAGTGAAGTTAATCATCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACtt                                                              >  1:1273153/1‑150 (MQ=255)
                                                                                        cccGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATg                                                          >  2:142879/1‑151 (MQ=255)
                                                                                            ccgGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAAt                                                      <  2:1283843/151‑1 (MQ=255)
                                                                                              gggTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATAc                                                    <  2:434277/151‑1 (MQ=255)
                                                                                               ggTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACg                                                   >  1:1279837/1‑151 (MQ=255)
                                                                                                gTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGt                                                  >  1:888489/1‑151 (MQ=255)
                                                                                                    gTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAGCAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCAt                                              <  2:750146/151‑1 (MQ=255)
                                                                                                    gTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCAt                                              <  2:866077/151‑1 (MQ=255)
                                                                                                     tGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATc                                             >  2:1220731/1‑151 (MQ=255)
                                                                                                        aGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAg                                          <  1:184040/151‑1 (MQ=255)
                                                                                                           tAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTc                                       >  2:159014/1‑151 (MQ=255)
                                                                                                               cACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCAcgc                                   >  2:1062053/1‑151 (MQ=255)
                                                                                                                 cccATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACgcgc                                 >  1:2211/1‑151 (MQ=255)
                                                                                                                  ccATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGcc                                <  1:1035224/151‑1 (MQ=255)
                                                                                                                    aTGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCgg                              >  2:288873/1‑151 (MQ=255)
                                                                                                                    aTGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCgg                              >  2:876560/1‑151 (MQ=255)
                                                                                                                     tGCATTCGGACAAACTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAACGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATCACTATGACTTAATGGACTACGTTCATCCAGTTCACGCGCCGGt                             >  1:335240/1‑151 (MQ=255)
                                                                                                                            ggACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGc                      >  1:349778/1‑151 (MQ=255)
                                                                                                                             gACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGcc                     >  2:32627/1‑151 (MQ=255)
                                                                                                                              aCAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGcc                     >  1:786774/1‑150 (MQ=255)
                                                                                                                                aaaGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAAc                  >  2:1419762/1‑151 (MQ=255)
                                                                                                                                        tcGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCa             <  1:825426/148‑1 (MQ=255)
                                                                                                                                        tcGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAg            <  1:1141678/149‑1 (MQ=255)
                                                                                                                                            cATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgt      >  2:604412/1‑151 (MQ=255)
                                                                                                                                                ttttTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAAc                  >  2:264693/1‑135 (MQ=255)
                                                                                                                                                ttttTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAAc                  <  1:264699/135‑1 (MQ=255)
                                                                                                                                                 ttttCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtagta  >  2:1052567/1‑150 (MQ=255)
                                                                                                                                                 ttttCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtagta  <  1:432365/150‑1 (MQ=255)
                                                                                                                                                 ttttCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtagta  >  2:990110/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AATAAACATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTA  >  NC_000913/1817955‑1818249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: